Abstract
Isozyme banding pattern was studied in Guinea grass (Panicum maximum Jacq.), a widely cultivated grass having good fodder value. Similarity among 63 accessions collected from diverse sources was worked out using five enzyme systems (SOD, GOT, ACP, Esterase and Peroxidase) following horizontal starch gel electrophoresis. Biochemical markers such as isozymes are useful supplements in identifying the genetic variation present in any crop. A total of 35 clear and unambiguous bands were used for analysis of which 8 bands were monomorphic. Polymorphism exhibited by 27 bands from all five enzyme systems indicate presence of considerable diversity in this species. The dendrogram generated after UPGMA and SAHN cluster analysis using Jaccard genetic distance showed that 63 accessions from diverse geographical locations could be grouped in three main clusters, of which two could further be divided into sub-clusters. Although the clusters comprised members from different locations, most of the accessions from similar geographical locations tended to cluster in same group.
Similar content being viewed by others
References
M.K. Aradhya L.K. Kee F.T. Zee R.M. Manshardt (1998) ArticleTitleGenetic variability in Macadamia Genet. Resour. Crop Evol. 45 18–32 Occurrence Handle10.1023/A:1008634103954
B. Assienan M. Noirot (1995) ArticleTitleIsozyme polymorphism and organization of the agamic complex of the Maximae (Panicum maximum Jacq., P. infestum Anders and P. trichocladeum K. Schum.) in Tanzania Theor. Appl. Genet. 91 672–680 Occurrence Handle10.1007/BF00223296 Occurrence Handle1:CAS:528:DyaK2MXpsVertbk%3D
B. Assienan M. Noirot Y. Gnagne (1993) ArticleTitleInheritance and genetic diversity of some enzymes in the sexual and diploid pool of the agamic complex of maximae (Panicum maximum Jacq., P. infestum Anders. and P. trichocladum K. Schum.) Euphytica 68 231–239 Occurrence Handle10.1007/BF00029877 Occurrence Handle1:CAS:528:DyaK2cXitFaktrg%3D
G.W. Burton J.C. Millot W.G. Monson (1973) ArticleTitleBreeding procedures for Panicum maximum Jacq. suggested by plant variability and mode of reproduction Crop Sci. 13 717–720
G.S.C. Buso P.H. Rangel M.E. Fereira (1998) ArticleTitleAnalysis of genetic variability of south American wild rice populations. (Oryza glumaepatula) with isozymes and RAPD markers Mol. Ecol. 7 107–117 Occurrence Handle10.1046/j.1365-294x.1998.00321.x Occurrence Handle1:CAS:528:DyaK1cXhtFSqu7o%3D
A. Jain S.N. Zadoo A.K. Roy P. Kaushal D.R. Malaviya (2003) ArticleTitleMeiotic system and probable basic chromosome number of Panicum maximum Jacq. Accessions Cytologia 68 IssueID1 7–13 Occurrence Handle10.1508/cytologia.68.7
M. Klaas (1998) ArticleTitleApplication and impact of molecular markers on evolutionary and diversity studies in Allium Plant Breeding 117 297–308 Occurrence Handle1:CAS:528:DyaK1cXmvFelu7k%3D
O. Lange M.T. Schiffino-Wittmann (2000) ArticleTitleIsozyme variation in wild and cultivated species of the genus Trifolium L. (Leguminosae) Ann Bot. 86 339–345 Occurrence Handle10.1006/anbo.2000.1190 Occurrence Handle1:CAS:528:DC%2BD3cXltlOqsL4%3D
D.R. Malaviya (1996) ArticleTitleDistribution of morphological diversity among germplasm lines of Panicum maximum Indian J. Pl. Genet. Resour. 9 193–196
N.A. Mantel (1967) ArticleTitleThe detection of disease clustering and a generalized regression approach Cancer Res. 27 209–220 Occurrence Handle1:STN:280:CCiD1MzgsVY%3D Occurrence Handle6018555
K. Nakajima (1978) ArticleTitleComparison of major agronomic characters in Guinea grass and colored Guinea grass JARQ 12 145–151
M.D. Poulik (1957) ArticleTitleStarch gel electrophoresis in a discontinuous system of buffers Nature 180 1477–1479 Occurrence Handle1:CAS:528:DyaG1cXjtlamsQ%3D%3D Occurrence Handle13493570
Y. Savidan J. Pernes (1982) ArticleTitleDiploid-tetraploid-dihaploid cycles and the evolution of Panicum maximum Jacq Evolution 36 596–600
Y.H. Savidan (1982) ArticleTitleEmbryological analysis of facultative apomixis in Panicum maximum Jacq Crop Sci. 22 467–469
Y.H. Savidan L. Jank J.C.G. Costa C.B. do Valle (1989) ArticleTitleBreeding Panicum maximum in Brazil. 1. Genetic resources, mode of reproduction and breeding procedures Euphytica 41 107–112 Occurrence Handle10.1007/BF00022419
R.L. Smith (1979) ArticleTitleSeed dormancy in Panicum maximum Jacq Trop. Agric. 56 233–239 Occurrence Handle1:CAS:528:DyaE1MXkvVOnsbg%3D
O. Smithies (1955) ArticleTitleZone electrophoresis in starch gel: group variation in the serum proteins of normal human adults Biochem. J. 61 629–641 Occurrence Handle1:CAS:528:DyaG28XitVChug%3D%3D Occurrence Handle13276348
J.A. Veech (1969) ArticleTitleLocalization of peroxidase in infected tobaccos susceptible and resistant to black shank Phytopathology 59 556–571
H.E. Warmke (1951) ArticleTitleCytotaxonomic investigations of some varieties of Panicum maximumof P. purpurascens in Puerto Rico Agron. J. 43 143–149
J.F. Wendel N.F. Weeden (1989) Visualization and interpretation of plant isozymes D.S. Soltis P.S. Soltis (Eds) Plant Biology Chapman and Hall London
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jain, A., Roy, A.K., Kaushal, P. et al. Isozyme Banding Pattern and Estimation of Genetic Diversity among Guinea Grass Germplasm. Genet Resour Crop Evol 53, 339–347 (2006). https://doi.org/10.1007/s10722-004-0574-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10722-004-0574-0