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Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells

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Abstract

In this study, we found that gene expression of the human β-galactoside α2,6-sialyltransferase (hST6Gal I) was specifically increased during differentiation of human MG-63 osteoblastic cells by serum starvation (SS). In parallel, a distinct increase in binding to SNA, the α2,6-sialyl-specific lectin, was observed in serum-starved cells, as demonstrated by FACS analysis. 5’-Rapid amplification of cDNA ends analysis demonstrated that the increase of hST6Gal I transcript by SS is mediated by P1 promoter. To elucidate transcriptional regulation of hST6Gal I in SS-induced MG-63 cells, we functionally characterized the P1 promoter region of the hST6Gal I gene. The 5’-deletion analysis of P1 promoter region revealed that the 189 bp upstream region of transcription start site is critical for transcriptional activity of hST6Gal I gene in SS-induced MG-63 cells. This region contains the predicted binding sites for several transcription factors, including AREB6, FOXP1, SIX3, HNF1, YY2, and MOK2. The mutagenesis analysis for these sites and chromatin immunoprecipitation assay demonstrated that the YY2 binding site at -98 to -77 was essential for the SS-induced hST6Gal I gene expression during differentiation of MG-63 cells.

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Acknowledgements

This work was supported by the Dong-A University research fund.

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Author notes

  1. So-Young An and Miri Lee contributed equally to this work.

Authors and Affiliations

  1. Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea

    So-Young An, Miri Lee, Hyun-Kyoung Yoon, Kyoung-Sook Kim, Dong-Hyun Kim & Young-Choon Lee

  2. Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do, 16419, South Korea

    Fukushi Abekura & Cheorl-Ho Kim

  3. Department of Orthopaedic Surgery, College of Medicine, Dong-A University, Busan, 49201, South Korea

    Hyeon-Jun Kim

  4. Functional Genomics Laboratory, Department of Animal Sciences, the Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210, USA

    Kichoon Lee

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  1. So-Young An
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Contributions

Conceived and designed the experiments: Miri Lee, Cheorl-Ho Kim, Young-Choon. Performed the experiments: Miri Lee, So-Young An, Hyun-Kyoung Yoon. Analyzed the data: Miri Lee, So-Young An, Hyun-Kyoung Yoon, Kyoung-Sook Kim, Dong-Hyun Kim, Cheorl-Ho Kim, Young-Choon Lee. Contributed reagents/materials/analysis tools: Dong-Hyun Kim, Hyun-Jun Kim, Kichoon Lee, Cheorl-Ho Kim. Wrote the paper: Miri Lee, Cheorl-Ho Kim, Young-Choon Lee.

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Correspondence to Cheorl-Ho Kim or Young-Choon Lee.

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An, SY., Lee, M., Yoon, HK. et al. Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells. Glycoconj J 37, 681–690 (2020). https://doi.org/10.1007/s10719-020-09959-3

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