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Novel interactions of complex carbohydrates with peanut (PNA), Ricinus communis (RCA-I), Sambucus nigra (SNA-I) and wheat germ (WGA) agglutinins as revealed by the binding specificities of these lectins towards mucin core-2 O-linked and N-linked glycans and related structures

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Abstract

Plant lectins through their multivalent quaternary structures bind intrinsically flexible oligosaccharides. They recognize fine structural differences in carbohydrates and interact with different sequences in mucin core 2 or complex-type N-glycan chain and also in healthy and malignant tissues. They are used in characterizing cellular and extracellular glycoconjugates modified in pathological processes. We study here, the complex carbohydrate-lectin interactions by determining the effects of substituents in mucin core 2 tetrasaccharide Galβ1-4GlcNAcβ1-6(Galβ1-3)GalNAcα-O-R and fetuin glycopeptides on their binding to agarose-immobilized lectins PNA, RCA-I, SNA-I and WGA. Briefly, in mucin core 2 tetrasaccharide (i) structures modified by α2-3/6-Sialyl LacNAc, LewisX and α1-3-Galactosyl LacNAc resulted in regular binding to PNA whereas compounds with 6-sulfo LacNAc displayed no-binding; (ii) strucures bearing α2-6-sialyl 6-sulfo LacNAc, or 6-sialyl LacdiNAc carbohydrates displayed strong binding to SNA-I; (iii) structures with α2-3/6-sialyl, α1-3Gal LacNAc or LewisX were non-binder to RCA-I and compounds with 6-sulfo LacNAc only displayed weak binding; (iv) structures containing LewisX, 6-Sulfo LewisX, α2-3/6-sialyl LacNAc, α2-3/6-sialyl 6-sulfo LacNAc and GalNAc Lewis-a were non-binding to WGA, those with α1-2Fucosyl, α1-3-Galactosyl LacNAc, α2-3-sialyl T-hapten plus 3ʹ/6ʹsulfo LacNAc displayed weak binding, and compounds with α2-3-sialyl T-hapten, α2.6-Sialyl LacdiNAc, α2-3-sialyl D-Fucβ1-3 GalNAc and Fucα-1-2 D-Fucβ-1-3GalNAc displaying regular binding and GalNAc LewisX and LacdiNAc plus D-Fuc β-1-3 GalNAcα resulting in tight binding. RCA-I binds Fetuin triantennary asialoglycopeptide 100 % after α-2-3 and 25 % after α-2-6 sialylation, 30 % after α-1-2 and 100 % after α-1-3 fucosylation, and 50 % after α-1-3 galactosylation. WGA binds 3-but not 6-Fucosyl chitobiose core. Thus, information on the influence of complex carbohydrate chain constituents on lectin binding is apparently essential for the potential application of lectins in glycoconjugate research.

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Abbreviations

AA/CP:

Acrylyamide copolymer

AAL:

Aleuria aurantia lectin

Al:

Allyl

Bn:

Benzyl

BGA:

Blood group antigen

BSA:

Bovine serum albumin

BSM:

Bovine submaxillary mucin

CGM:

Cowper’s gland mucin

CMP:

Cytidine 5′ monophosphate

FOG:

Fetuin O-glycosidic

FTA:

Fetuin triantennary

GP:

Glycopeptide

LacNAc:

Galβ1, 4GlcNAc

Me:

Methyl

Mucin Core 2:

Galβ1-3(GlcNAcβ1-6)GalNAcα-o-Ser/Thr

PGM:

Pig gastric mucin

PNA:

Peanut agglutinin

RCA-I:

Ricinus communis agglutinin-I

SNA-I:

Sambucus nigra agglutinin-I

ST:

Sialyltransferase

T-hapten:

Galβ1-3GalNAcα-O-Ser/Thr

Tn-hapten:

GalNAcα-O-Ser/Thr

WGA:

Wheat germ agglutinin

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Acknowledgments

The study was supported by NIH Grants CA 35329; HL103411 and DOD grant W81XWH-06-1-0013.

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Authors and Affiliations

  1. Department of Cancer Biology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA

    E.V. Chandrasekaran, Jun Xue, Jie Xia, Siraj D. Khaja, Conrad F. Piskorz, Robert D. Locke & Khushi L. Matta

  2. Department of Chemical and Biological Engineering, State University of New York, Buffalo, NY, 14260, USA

    Sriram Neelamegham & Khushi L. Matta

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  1. E.V. Chandrasekaran
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Chandrasekaran, E., Xue, J., Xia, J. et al. Novel interactions of complex carbohydrates with peanut (PNA), Ricinus communis (RCA-I), Sambucus nigra (SNA-I) and wheat germ (WGA) agglutinins as revealed by the binding specificities of these lectins towards mucin core-2 O-linked and N-linked glycans and related structures. Glycoconj J 33, 819–836 (2016). https://doi.org/10.1007/s10719-016-9678-y

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