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Polysaccharide-rich fraction of Termitomyces eurhizus accelerate healing of indomethacin induced gastric ulcer in mice

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Abstract

The current study aims to determine the healing activity of water soluble polysaccharide-rich fraction of a wild mushroom, Termitomyces eurhizus (TEps) against the indomethacin induced gastric ulceration in mice model. Gastric tissue histology, myeloperoxidase (MPO) activity, cyclooxygenases (COX) 1 and 2 expression, prostaglandin E2 (PGE2) synthesis, and modulation of pro/anti inflammatory cytokines expression were studied for this purpose. Histological study shows that TEps (20 mg/kg) effectively healed the gastric ulceration. Based on biochemical results, the healing capacities of TEps could be attributed to reduction of MPO activity and protection of mucosal mucin content. Enhanced synthesis of PGE2 by modulation of COX-1 and COX-2 expression and a prominent shift of cytokines expression from pro (TNF-α, IL-1ß) to anti inflammatory (IL-10) side are also held responsible for ulcer healing. The preliminary study highlights the anti-ulcerogenic property of polysaccharide-rich fraction of Termitomyces eurhizus and opens an alternative cure for NSAID induced gastroduodenal diseases.

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Acknowledgement

Authors like to acknowledge Director, I.P.G.M.E&R, Kol-20 and Director, KPCMC&H, Kol-32 for their kind support and help. Ananya Chatterjee is thankful to Board of Research in Nuclear Science (BRNS), Department of Atomic Energy (DAE), GOI India for providing fellowship. The work was financially supported by BRNS, DAE, GOI.

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Authors and Affiliations

  1. Central Research Laboratory, Department of Biochemistry, KPC Medical College and Hospital, 1F, Raja S.C. Mullick Road, Jadavpur, Kolkata, 700032, West Bengal, India

    Ananya Chatterjee, Sirshendu Chatterjee, Shatavisa Mukherjee, Atashi Mukherjee & Sandip K. Bandyopadhyay

  2. Research Wing, Department of Biochemistry, University College of Medicine, I.P.G.M.E&R, 244B A.J.C. Bose Road, Kolkata, 700020, West Bengal, India

    Ananya Chatterjee & Sandip K. Bandyopadhyay

  3. Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, West Bengal, 700019, India

    Ananya Chatterjee, Somanjana Khatua, Soumitra Paloi & Krishnendu Acharya

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  1. Ananya Chatterjee
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  2. Somanjana Khatua
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  3. Sirshendu Chatterjee
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Correspondence to Sandip K. Bandyopadhyay.

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Highlights

1. Healing activity of water soluble polysaccharide-rich fraction of Termitomyces eurhizus (TEps) against the indomethacin induced gastric ulceration in mice model is proposed.2. Histological study shows that TEps (20 mg/kg) effectively healed the gastric ulceration.3. Reduction of MPO activity, protection of mucosal mucin content, enhanced synthesis of PGE2 by modulation of COX-1 and COX-2 expression and balance between pro inflammatory (TNF-α, IL-1ß) and anti inflammatory cytokines (IL-10) are responsible for ulcer healing.4. The preliminary study uncovers the anti-ulcerogenic property of TEps.

Appendix

Appendix

TEps, Polysaccharide-rich fraction of Termitomyces eurhizus; NSAID, Non Steroidal Anti Inflammatory Drug; PG, Prostaglandin; ELISA, Enzyme Linked Immunosorbent Assay; UI, Ulcer Index; TNF-a , Tumour Necrosis Factor , alpha ; IL , Interleukin; Omp, Omeprazole; g, Gram.

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Chatterjee, A., Khatua, S., Chatterjee, S. et al. Polysaccharide-rich fraction of Termitomyces eurhizus accelerate healing of indomethacin induced gastric ulcer in mice. Glycoconj J 30, 759–768 (2013). https://doi.org/10.1007/s10719-013-9479-5

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