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A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica

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Abstract

A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from fresh fruiting bodies of the wild mushroom Russula delica. The lectin was composed of two identical subunits, each with a molecular weight of 30 kDa. It was adsorbed on both SP-Sepharose and Q-Sepharose and unadsorbed on DEAE-cellulose. Its hemagglutinating activity was stable up to 70°C, and in HCl and NaOH solutions of concentrations up to 25 and 12.5 mM, respectively. The activity was inhibited by inulin and o-nitrophenyl-β-D-galactopyranoside. Al3+, Fe3+ and Zn2+ ions, but not by Ca2+, Mg2+ and Mn2+ ions. Mg2+ ions at 10 mM concentration potentiated the hemagglutinating activity of the lectin. Russula delica lectin was devoid of mitogenic activity toward mouse splenocytes, but potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.88 µM and 0.52 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.26 μM.

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Acknowledgments

This work was financially supported by National Grants of China (nyhyzx07-008 and 2006BAD07A01).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100094, China

    Shuang Zhao, Jingkun Zhao, Guoqing Zhang & Hexiang Wang

  2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Shuang Zhao

  3. Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming, 650223, China

    Yongchang Zhao & Shuhong Li

  4. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    Tzi Bun Ng

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  1. Shuang Zhao
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  2. Yongchang Zhao
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  3. Shuhong Li
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  4. Jingkun Zhao
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  5. Guoqing Zhang
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  6. Hexiang Wang
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  7. Tzi Bun Ng
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Correspondence to Hexiang Wang or Tzi Bun Ng.

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Submitted for publication to Glycoconjugate Journal July 2009.

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Zhao, S., Zhao, Y., Li, S. et al. A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica . Glycoconj J 27, 259–265 (2010). https://doi.org/10.1007/s10719-009-9274-5

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  • DOI: https://doi.org/10.1007/s10719-009-9274-5

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