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Sulfated hyaluronan derivatives reduce the proliferation rate of primary rat calvarial osteoblasts

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Abstract

Glycosaminoglycans (GAG) and proteoglycans, which are components of the extracellular bone matrix, are also localized in and at the membrane of osteoblasts and in the pericellular matrix. Due to their interaction with several growth factors, water and cations these molecules play an important role in regulating proliferation and differentiation of osteoblasts and bone development. The aim of this study was to assess in vitro the effects of two chemically sulfated hyaluronan (HyaS) derivatives on the proliferation of rat calvarial osteoblasts and to compare with those of native hyaluronan (Hya) and natural sulfated GAG such as chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), dermatan sulfate (DS) and heparan sulfate (HS). Moderately and highly sulfated HyaS derivatives caused a time-dependent reduction of osteoblast proliferation. The anti-proliferative effect of HyaS was accompanied by a cell cycle arrest in the G1 phase, but was not associated with cell death. Whereas non-sulfated high molecular weight (HMW)- and low molecular weight (LMW)-Hya as well as C4S, C6S, DS and HS showed no effect on the cell proliferation.

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Abbreviations

GAG:

glycosaminoglycans

HyaS:

sulfated hyaluronan

CS:

chondroitin sulfate

DS:

dermatan sulfate

HS:

heparan sulfate

HMW:

high molecular weight

LMW:

low molecular weight

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Acknowledgement

The authors thank the Deutsche Forschungsgemeinschaft for financial support (grant HE2763/5-1 & SCHN1099/1-1 & TR67), and Christine Kupke and Carolin Preissler for technical assistance.

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Authors and Affiliations

  1. Institute of Physiological Chemistry, Dresden University of Technology, Fiedlerstrasse 42, 01307, Dresden, Germany

    Reiner Kunze, Manuela Rösler, Ute Hempel & Peter Dieter

  2. Biomaterials Department, INNOVENT e.V., Prüssingstrasse 27 B, 07745, Jena, Germany

    Stephanie Möller & Matthias Schnabelrauch

  3. Institute of Medical Physics and Biophysics, University Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany

    Thomas Riemer

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  1. Reiner Kunze
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  2. Manuela Rösler
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  4. Matthias Schnabelrauch
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Correspondence to Reiner Kunze.

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Kunze, R., Rösler, M., Möller, S. et al. Sulfated hyaluronan derivatives reduce the proliferation rate of primary rat calvarial osteoblasts. Glycoconj J 27, 151–158 (2010). https://doi.org/10.1007/s10719-009-9270-9

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  • DOI: https://doi.org/10.1007/s10719-009-9270-9

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