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Preparation of homogenous oligosaccharide chains from glycosphingolipids

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Abstract

After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

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Acknowledgements

This work was supported by NIH Grant Number R01NS009626 from the National Institute of Neurological Disorders and stroke (to Y.T.L) and Basic Research B 1938006 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (to M.I).

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Authors and Affiliations

  1. Department of Biochemistry, Tulane University Health Sciences Center School of Medicine, New Orleans, LA, 70112, USA

    Yu-Teh Li & Su-Chen Li

  2. Proteomics Core Facility, Department of Physiology, Louisiana State University Health Sciences Center School of Medicine, New Orleans, LA, 70112, USA

    Chau-Wen Chou

  3. Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenviromental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Utaro Kobayashi, Yo-hey Ishibashi & Makoto Ito

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  1. Yu-Teh Li
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  2. Chau-Wen Chou
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  3. Su-Chen Li
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  4. Utaro Kobayashi
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  5. Yo-hey Ishibashi
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  6. Makoto Ito
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Correspondence to Yu-Teh Li.

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Li, YT., Chou, CW., Li, SC. et al. Preparation of homogenous oligosaccharide chains from glycosphingolipids. Glycoconj J 26, 929–933 (2009). https://doi.org/10.1007/s10719-008-9125-9

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  • DOI: https://doi.org/10.1007/s10719-008-9125-9

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