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Chromosomal organization of repetitive DNA sequences in Astyanax bockmanni (Teleostei, Characiformes): dispersive location, association and co-localization in the genome

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Abstract

Repetitive DNA sequences constitute a great portion of the genome of eukaryotes and are considered key components to comprehend evolutionary mechanisms and karyotypic differentiation. Aiming to contribute to the knowledge of chromosome structure and organization of some repetitive DNA classes in the fish genome, chromosomes of two allopatric populations of Astyanax bockmanni were analyzed using classic cytogenetics techniques and fluorescent in situ hybridization, with probes for ribosomal DNA sequences, histone DNA and transposable elements. These Astyanax populations showed the same diploid number (2n = 50), however with differences in chromosome morphology, distribution of constitutive heterochromatin, and location of 18S rDNA and retroelement Rex3 sites. In contrast, sites for 5S rDNA and H1, H3 and H4 histones showed to be co-located and highly conserved. Our results indicate that dispersion and variability of 18S rDNA and heterochromatin sites are not associated with macro rearrangements in the chromosome structure of these populations. Similarly, distinct evolutionary mechanisms would act upon histone genes and 5S rDNA, contributing to chromosomal association and co-location of these sequences. Data obtained indicate that distinct mechanisms drive the spreading of repetitive DNAs in the genome of A. bockmanni. Also, mobile elements may account for the polymorphism of the major rDNA sites and heterochromatin in this genus.

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Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.

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Authors and Affiliations

  1. Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, s/n, Botucatu, SP, 18618-970, Brazil

    Duílio M. Z. A. Silva, José Carlos Pansonato-Alves, Ricardo Utsunomia, Claudio Oliveira & Fausto Foresti

  2. Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista, Campus de Bauru, Bauru, SP, 17033-360, Brazil

    Sandro Natal Daniel & Fabio Porto-Foresti

  3. CAUNESP, Universidade Estadual Paulista, Campus Jaboticabal, Jaboticabal, SP, 14884-900, Brazil

    Diogo Teruo Hashimoto

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  1. Duílio M. Z. A. Silva
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  2. José Carlos Pansonato-Alves
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  3. Ricardo Utsunomia
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Correspondence to Duílio M. Z. A. Silva.

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Silva, D.M.Z.A., Pansonato-Alves, J.C., Utsunomia, R. et al. Chromosomal organization of repetitive DNA sequences in Astyanax bockmanni (Teleostei, Characiformes): dispersive location, association and co-localization in the genome. Genetica 141, 329–336 (2013). https://doi.org/10.1007/s10709-013-9732-7

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