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Allelic variation, balancing selection and positive selected sites detected from MHC class Iα gene of olive flounder

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Abstract

Major histocompatibility complex (MHC) genes play an important role in the immune response of vertebrates. Allelic polymorphism and pattern of evolution in MHC genes has been investigated in many mammals, however, much less is known in teleost. In the present study, we have investigated complete MHC Iα gene consists of 7 exons and 6 introns in Olive flounder (Paralichthys olivaceus). Genetic variation in the MHC class Iα gene was also tested in flounder. In 32 individuals, a total of 62 alleles were detected from exon 2 of MHC class Iα gene. The rate of non-synonymous substitutions (d N ) occurred at a significantly higher frequency than that of synonymous substitutions (d S ) in PBR and non-PBR, suggesting that balancing selection for maintaining polymorphisms at the MHC Iα locus. Many positive selection sites were found to act very intensively on antigen binding sites. Our founding suggests a snapshot in an evolutionary process of MHC Iα gene evolution of the P. olivaceus.

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Acknowledgments

This work was supported by National Major Basic Research Program (973) of china (2010CB126303), National sparking plan project (2010GA700010), Important Science and Technology Specific Projects of Zhejiang Province (2009C12078),Foundation of Zhoushan City (092031), Open Foundation from Ocean Fishery Science and Technology in the Most Important Subjects of Zhejiang (20100111).

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Authors and Affiliations

  1. Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, 316000, Zhoushan, China

    Tian-jun Xu & Yue-na Sun

  2. Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071, Qingdao, China

    Song-lin Chen

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  1. Tian-jun Xu
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  2. Yue-na Sun
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Correspondence to Song-lin Chen.

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Xu, Tj., Sun, Yn. & Chen, Sl. Allelic variation, balancing selection and positive selected sites detected from MHC class Iα gene of olive flounder. Genetica 138, 1251–1259 (2010). https://doi.org/10.1007/s10709-010-9524-2

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  • DOI: https://doi.org/10.1007/s10709-010-9524-2

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