Skip to main content
SpringerLink
Log in

Chromosomal localization of 18S and 5S rDNA using FISH in the genus Tor (Pisces, Cyprinidae)

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Dual color fluorescence in situ hybridization (FISH) was performed to study the simultaneous chromosomal localization of 18S and 5S ribosomal genes in the genus Tor for the first time. The 18S and 5S rDNAs in four Tor species were amplified, sequenced and mapped on the metaphase chromosomes. The number and distribution of 18S and 5S rDNA clusters were examined on metaphase chromosome spreads using FISH. The specimens of T. chelynoides, T. putitora and T. progeneius showed six bright fluorescent signals of 18S rDNA and T. tor exhibited ten such signals. The 5S rDNA signals were present only on one pair of chromosomes in all the four Tor species. Ag-NORs were observed on two pairs of chromosomes in T. chelynoides, T. putitora, T. progeneius and four pairs in T. tor. Comparison of the observed 18S rDNA FISH signals and Ag-NORs strongly suggested a possible inactivation of NORs localized at the telomeres of a subtelocentric and telocentric chromosome pairs in all four species. The 5S rDNA contained an identical 120 bp long coding region and 81 bp long highly divergent non-transcribed spacers in all species examined. 18S and 5S rDNA sequencing and chromosomal localization can be a useful genetic marker in species identification as well as phylogenetic and evolutionary studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Almeida-Toledo LF, Ozouf-Costaz C, Foresti F, Bonillo C, Porto-Foresti F, Daniel-Silva MZF (2002) Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae). Cytogenet Genome Res 97(3–4):229–233. doi:10.1159/000066609

    Article  CAS  PubMed  Google Scholar 

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. doi:10.1093/nar/25.17.3389

    Article  CAS  PubMed  Google Scholar 

  • Bertollo LAC, Takahashi CS, Moreira-Filho O (1978) Cytotaxonomic consideration on Hoplias lacrdae (Pisces, Erythrinidae). Braz J Genet 1:103–120

    Google Scholar 

  • Cabrero J, Camacho JP (2008) Location and expression of ribosomal RNA genes in grasshoppers: abundance of silent and cryptic loci. Chromosom Res 16(4):595–607. doi:10.1007/s10577-008-1214-x

    Article  CAS  Google Scholar 

  • Ferro DAM, Neo DM, Moreira-Filho O, Bertollo LAC (2001) Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): population distribution and functional diversity. Genetica 110:55–62. doi:10.1023/A:1017963217795

    Article  Google Scholar 

  • Fontana F, Lanfredi M, Congiu L, Leis M, Chicca M, Rossi R (2003) Chromosomal mapping of 18S–28S and 5S rRNA genes by two-colour fluorescent in situ hybridization in six sturgeon species. Genome 46:473–477. doi:10.1139/g03-007

    Article  CAS  PubMed  Google Scholar 

  • Fujiwara A, Abe S, Yamaha E, Yamazaki F, Yoshida MC (1998) Chromosomal localization and heterochromatin association of ribosomal RNA genes loci and silver stained nucleolar organizer regions in salmonid fishes. Chromosom Res 6:463–471. doi:10.1023/A:1009200428369

    Article  CAS  Google Scholar 

  • Gromicho M, Coutanceau JP, Ozouf-Costaz C, Collares-Pereira MJ (2006) Contrast between extensive variation of 28S rDNA and stability of 5S rDNA and telomeric repeats in the diploid-polyploid Squalius alburnoides complex and in its maternal ancestor Squalius pyrenaicus (Teleostei, Cyprinidae). Chromosom Res 14:297–306. doi:10.1007/s10577-006-1047-4

    Article  CAS  Google Scholar 

  • Guillén AK, Hirai Y, Tanoue T, Hirai H (2004) Transcriptional repression mechanisms of nucleolus organizer regions (NORs) in humans and chimpanzees. Chromosom Res 12(3):225–237. doi:10.1023/B:CHRO.0000021911.43225.eb

    Article  Google Scholar 

  • Hallenberg C, Nederby-Nielson J, Frederiksen S (1994) Characterization of 5S rRNA genes from mouse. Gene Amst 142:291–295. doi:10.1016/0378-1119(94)90277-1

    Article  CAS  Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a one step method. Experementia 36:1014–1015. doi:10.1007/BF01953855

    Article  CAS  Google Scholar 

  • Inafuku J, Nabeyama M, Kikuma Y, Saitoh J, Kubota S, Kohno S (2000) Chromosomal location and nucleotide sequences of 5S ribosomal DNA of two cyprinid species (Osteichthyes, Pisces). Chromosom Res 8:193–199. doi:10.1023/A:1009292610618

    Article  CAS  Google Scholar 

  • Khuda-Bukhsh AR, Nayak K (1982) Karyomorpological studies in two species of hill stream fishes from Kashmir, India: occurrence of a high number of chromosomes. Chromosom inf Sci (Japan) 33:12–14

    Google Scholar 

  • Kushwaha B, Srivastava SK, Nagpure NS, Ogale SN, Ponniah AG (2001) Cytogenetic studies in two species of mahseer, Tor khudree and Tor mussullah (Cyprinidae, Pisces) from India. Chromosom Sci 5:47–50

    Google Scholar 

  • Lakra WS (1996) Cytogenetic studies on endangered fish species: karyotypes of three species of mahseers, Tor putitora, T. tor and T. khudree (Cyprinidae Pisces). Cytobios 85:205–218

    Google Scholar 

  • Long EO, David ID (1980) Repeated genes in eukaryotes. Annu Rev Biochem 49:727–764. doi:10.1146/annurev.bi.49.070180.003455

    Article  CAS  PubMed  Google Scholar 

  • Lucchini S, Nardi I, Barsacchi G, Batistoni R, Andronico F (1993) Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome 36:762–773. doi:10.1139/g93-101

    Article  CAS  PubMed  Google Scholar 

  • Mantovani M, Abel LDS, Moreira-Filho O (2005) Conserved 5S and variable 45S rDNA chromosomal localization revealed by FISH in Astyanax scabripinnis (Pisces, Characidae). Genetica 123:211–216. doi:10.1007/s10709-004-2281-3

    Article  CAS  PubMed  Google Scholar 

  • Martinez JL, Moran P, Garcia-Vazquez S, Pendas AM (1996) Chromosomal localization of major and 5S rRNA genes in the European eel (Anguilla anguilla). Cytogenet Cell Genet 73:149–152

    Article  CAS  PubMed  Google Scholar 

  • Martins C, Galetti PM Jr (1999) Chromosomal localization of 5S rRNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosom Res 7:363–367. doi:10.1023/A:1009216030316

    Article  CAS  Google Scholar 

  • Martins C, Galetti PM (2000) Conservative distribution of 5S rDNA loci in Schizodon (Pisces, Anastomidae) chromosomes. Chromosom Res 8:353–355. doi:10.1023/A:1009243815280

    Article  CAS  Google Scholar 

  • Martins C, Galetti PM Jr (2001) Two 5S rDNA array in neotropical fish species: is it a general rule for fishes? Genetica 111:439–446. doi:10.1023/A:1013799516717

    Article  CAS  PubMed  Google Scholar 

  • Martins C, Wasko AP (2004) Organization and evolution of 5S ribosomal DNA in the fish genome. In: Williams CR (ed) Focus on Genome Research. Nova Science Publishers, Hauppauge, pp 289–319

    Google Scholar 

  • Moran P, Martinez JL, Garcia-Vazquez S, Pendas AM (1996) Sex linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet 75:145–150. doi:10.1159/000134466

    Article  CAS  PubMed  Google Scholar 

  • Nederby-Nielson J, Hallenberg C, Frederiksen S, Sorensen PD, Lomholt B (1993) Transcription of human 5S rRNA genes is influenced by an upstream DNA sequence. Nucleic Acids Res 26:3631–3636

    Google Scholar 

  • Pendas AM, Moran P, Garcia-Vazquez E (1993) Multi chromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chromosom Res 1:63–67. doi:10.1007/BF00710608

    Article  CAS  Google Scholar 

  • Pendas AM, Moran P, Freije JP, Garcia-Vazquez S (1994) Chromosomal mapping and nucleotide sequences of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet Cell Genet 67:31–36. doi:10.1159/000133792

    Article  CAS  PubMed  Google Scholar 

  • Sajdak SL, Reed KM, Phillips RB (1998) Intra individual and interspecies variation in the 5S rDNA of coregonid fish. J Mol Evol 46:680–688. doi:10.1007/PL00006348

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell I (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Plainsveiw New York

    Google Scholar 

  • Santos LVDR, Foresti F, Wasko AP, Oliveira C, Martins C (2006) Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes). Genet Mol Biol 29(2):251–256. doi:10.1590/S1415-47572006000200009

    Article  Google Scholar 

  • Sarkar UK, Srivastava SM (2000) Endangered Mahseer in North East India. In: Ponniah AG, Sarkar UK (eds) Fish Biodiversity of North East India, NBFGR, NATP publ, Lucknow, India, pp 95–96

  • Sola L, Rossi AR, Iaselli V, Rasch EM, Monaco PJ (1992) Cytogenetics of bisexual/unisexual species of Poecilia mexicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenet Cell Genet 60:119–235

    Article  Google Scholar 

  • Suzuki H, Sakurai S, Matsuda Y (1996) Rat 5S rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19. Cytogenet Cell Genet 72:1–4. doi:10.1159/000134149

    Article  CAS  PubMed  Google Scholar 

  • Tigano C, Rocco L, Ferrito V, Costagliola D, Pappalardo AM, Stinso V (2004) Chromosomal mapping and molecular characterization of ribosomal RNA genes in Lebias fasciata (Teleostei, Cyprinodontidae). Genetica 121:95–100. doi:10.1023/B:GENE.0000019931.89458.dc

    Article  CAS  PubMed  Google Scholar 

  • Wasko AP, Martins C, Wright JM, Galetti PM (2001) Molecular organization of 5S rDNA in fishes of genus Brycon. Genome 44:893–902. doi:10.1139/gen-44-5-893

    Article  CAS  PubMed  Google Scholar 

  • Winterfeld G, Roser M (2007) Deposition of ribosomal DNAs in the chromosome of perennial oats (Poaceae: Aveneae). Bot J Linn Soc 155:193–210. doi:10.1111/j.1095-8339.2007.00690.x

    Article  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Department of Biotechnology, the Ministry of Science and Technology, Government of India for funding.

Author information

Authors and Affiliations

  1. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O- Dilkusha, Lucknow, 226 002, Uttar Pradesh, India

    Mamta Singh, Ravindra Kumar, N. S. Nagpure, B. Kushwaha, Indramani Gond & W. S. Lakra

Authors
  1. Mamta Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ravindra Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. S. Nagpure
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Kushwaha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Indramani Gond
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. W. S. Lakra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ravindra Kumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, M., Kumar, R., Nagpure, N.S. et al. Chromosomal localization of 18S and 5S rDNA using FISH in the genus Tor (Pisces, Cyprinidae). Genetica 137, 245–252 (2009). https://doi.org/10.1007/s10709-009-9367-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-009-9367-x

Keywords

Search

Navigation