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Nucleotide polymorphism in the drought responsive gene Asr2 in wild populations of tomato

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Abstract

The Asr gene family (named after abscicic acid [ABA], stress, ripening), exclusively present in plant genomes, is involved in transcriptional regulation. Its members are up-regulated in roots and leaves of water- or salt-stressed plants. In previous work, evidence of adaptive evolution (as inferred from synonymous and nonsynonymous divergence rates) has been reported for Asr2 in Solanum chilense and S. arcanum, two species dwelling in habitats with different precipitation regimes. In this paper we investigate patterns of intraspecific nucleotide variation in Asr2 and the unlinked locus CT114 in S. chilense and S. arcanum. The extent of nucleotide diversity in Asr2 differed between species in more than one order of magnitude. In both species we detected evidence of non-neutral evolution, which may be ascribed to different selective regimes, potentially associated to unique climatic features, or, alternatively, to demographic events. The results are discussed in the light of demographic and selective hypotheses.

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Acknowledgements

We thank the Tomato Genetics Resource Center (University of California, Davis, USA) for providing the seeds of the tomato wild populations and Gustavo Gudesblat for his valuable help with the experimental work in the laboratory. We also wish to thank Romina Piccinali and David Ardell for their help with technical issues of software usage, and two anonymous reviewers for their constructive comments and criticisms on a previous version of this manuscript. This work was supported by grants from Universidad de Buenos Aires (UBA), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. M.G. held a fellowship from UBA. N.F. held a fellowship from CONICET. N.D.I. and E.H. are members of the Carrera del Investigador Científico, CONICET, Argentina.

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Authors and Affiliations

  1. Departamento de Fisiología, Biología Molecular y Celular and IFIByNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina

    Mariano I. Giombini, Nicolás Frankel & Norberto D. Iusem

  2. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina

    Esteban Hasson

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  1. Mariano I. Giombini
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  2. Nicolás Frankel
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  3. Norberto D. Iusem
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  4. Esteban Hasson
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Correspondence to Esteban Hasson.

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Giombini, M.I., Frankel, N., Iusem, N.D. et al. Nucleotide polymorphism in the drought responsive gene Asr2 in wild populations of tomato. Genetica 136, 13–25 (2009). https://doi.org/10.1007/s10709-008-9295-1

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  • DOI: https://doi.org/10.1007/s10709-008-9295-1

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