Abstract
A genetic map based on microsatellite polymorphisms and visible mutations of the Mediterranean fruit fly (medfly), Ceratitis capitata is presented. Genotyping was performed on single flies from several backcross families. The map is composed of 67 microsatellites and 16 visible markers distributed over four linkage groups. Fluorescence in situ hybridization of selected microsatellite markers on salivary gland polytene chromosomes allowed the alignment of these groups to the second, fourth, fifth and sixth chromosome. None of the markers tested showed segregation either with the X or the third chromosome. However, this map constitutes a substantial starting point for a detailed genetic map of C. capitata. The construction of an integrated map covering the whole genome should greatly facilitate genetic studies and future genome sequence projects of the species.
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Acknowledgments
We thank I. Papaggeli and K.Oikonomou for support in genotyping and in situ hybridization and G. Franz and J. Cladera for providing medfly strains. E. E. S. was supported by a Fellowship from the National Fellowship Foundation (Greece) and K. D. M. by a grant from European Committee, contract No ERBFMBICT 983067. This work was supported by grants from the Hellenic General Secretariat of Research and Technology (97EL-17) and University of Patras, Greece. The two anonymous reviewers are greatly appreciated for their useful suggestions.
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Stratikopoulos, E.E., Augustinos, A.A., Petalas, Y.G. et al. An integrated genetic and cytogenetic map for the Mediterranean fruit fly, Ceratitis capitata, based on microsatellite and morphological markers. Genetica 133, 147–157 (2008). https://doi.org/10.1007/s10709-007-9195-9
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DOI: https://doi.org/10.1007/s10709-007-9195-9