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Chromosome banding in three species of Hypsiboas (Hylidae, Hylinae), with special reference to a new case of B-chromosome in anuran frogs and to the reduction of the diploid number of 2n = 24 to 2n = 22 in the genus

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Abstract

The chromosomes of hylids Hypsiboas albopunctatus, H. raniceps, and H. crepitans from Brazil were analyzed with standard and differential staining techniques. The former species presented 2n = 22 and 2n = 23 karyotypes, the odd diploid number is due to the presence of an extra element interpreted as B chromosome. Although morphologically very similar to the small-sized chromosomes of the A complement, the B was promptly recognized, even under standard staining, on the basis of some characteristics that are usually attributed to this particular class of chromosomes. The two other species have 2n = 24, which is the chromosome number usually found in the species of Hypsiboas karyotyped so far. This means that 2n = 22 is a deviant diploid number, resulted from a structural rearrangement, altering the chromosome number of 2n = 24 to 2n = 22. Based on new chromosome data, some possibilities were evaluated for the origin of B chromosome in Hypsiboas albopunctatus, as well as the karyotypic evolution in the genus, leading to the reduction in the diploid number of 2n = 24 to 2n = 22.

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Acknowledgements

The authors are greatly indebted to FAPESP and CNPq for financial support; to Dr. A.S. Abe and Dr. P.C. Garcia for helping in the collection of some specimens used in this study; and to Rogilene Aparecida Prado for technical assistance.

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Authors and Affiliations

  1. Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Av. 24A, 1515, 13506-900, Rio Claro, SP, Brasil

    Simone Lilian Gruber & Sanae Kasahara

  2. Departamento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brasil

    Célio Fernando Baptista Haddad

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  1. Simone Lilian Gruber
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  2. Célio Fernando Baptista Haddad
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  3. Sanae Kasahara
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Correspondence to Sanae Kasahara.

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Supported by FAPESP and CNPq

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Gruber, S.L., Haddad, C.F.B. & Kasahara, S. Chromosome banding in three species of Hypsiboas (Hylidae, Hylinae), with special reference to a new case of B-chromosome in anuran frogs and to the reduction of the diploid number of 2n = 24 to 2n = 22 in the genus. Genetica 130, 281–291 (2007). https://doi.org/10.1007/s10709-006-9105-6

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  • DOI: https://doi.org/10.1007/s10709-006-9105-6

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