Skip to main content
SpringerLink
Log in

Interplasmid transposition demonstrates piggyBac mobility in vertebrate species

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

The piggyBac transposon is an extremely versatile helper-dependent vector for gene transfer and germ line transformation in a wide range of invertebrate species. Analyses of genome sequencing databases have identified piggyBac homologues among several sequenced animal genomes, including the human genome. In this report we demonstrate that this insect transposon is capable of transposition in primate cells and embryos of the zebrafish, Danio rerio. piggyBac mobility was demonstrated using an interplasmid transposition assay that has consistently predicted the germ line transformation capabilities of this mobile element in several other species. Both transfected COS-7 primate cells and injected zebrafish embryos supported the helper-dependent movement of tagged piggyBac element between plasmids in the characteristic cut-and-paste, TTAA target-site specific manner. These results validate piggyBac as a valuable tool for genetic analysis of vertebrates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • T. Aoki N. Noguchi M. Sasatsu M. Kono (1987) ArticleTitleComplete nucleotide sequence of pTZ12, a chloramphenicol-resistance plasmid of Bacillus subtilis Gene 51 107–111 Occurrence Handle3110008 Occurrence Handle1:CAS:528:DyaL2sXltFejt7g%3D Occurrence Handle10.1016/0378-1119(87)90481-1

    Article  PubMed  CAS  Google Scholar 

  • A. Amsterdam N. Hopkins (1999) ArticleTitleRetrovirus-mediated insertional mutagenesis in zebrafish Methods Cell Biol. 60 87–98 Occurrence Handle9891332 Occurrence Handle1:CAS:528:DyaK1MXmsVCiug%3D%3D Occurrence Handle10.1016/S0091-679X(08)61895-6

    Article  PubMed  CAS  Google Scholar 

  • C.P. Bonin R.S. Mann (2004) ArticleTitleA piggyBac transposon gene trap for the analysis of gene expression and function in Drosophila Genetics 167 1801–1811 Occurrence Handle15342518 Occurrence Handle1:CAS:528:DC%2BD2cXos1egsL8%3D Occurrence Handle10.1534/genetics.104.027557

    Article  PubMed  CAS  Google Scholar 

  • Bron, R., 1995. Plasmids, pp. 75–174 in Molecular Biological Methods for Bacillus, edited by C.R. Harwood & S.M. Cutting. John Wiley and Sons, New York

  • L.C. Cary M.J. Goebel B. Corsaro H.G. Wang E. Rosen M.J. Fraser (1989) ArticleTitleTransposon mutagenesis of Baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses Virology 172 156–169 Occurrence Handle2549707 Occurrence Handle1:CAS:528:DyaK3cXksFehs7s%3D Occurrence Handle10.1016/0042-6822(89)90117-7

    Article  PubMed  CAS  Google Scholar 

  • C.J. Coates C.L. Turney M. Frommer D.A. O’Brochta P.W. Atkinson (1997) ArticleTitleInterplasmid transposition of the mariner transposable element in non-drosophilid insects Mol. Gen. Genet. 253 728–733 Occurrence Handle9079884 Occurrence Handle1:CAS:528:DyaK2sXisV2nt7o%3D Occurrence Handle10.1007/s004380050377

    Article  PubMed  CAS  Google Scholar 

  • C.J. Coates C.L. Turney M. Frommer D.A. O’Brochta W.D. Warren P.W. Atkinson (1995) ArticleTitleThe transposable element mariner can excise in non-drosophilid insects Mol. Gen. Genet. 249 246–252 Occurrence Handle7500947 Occurrence Handle1:CAS:528:DyaK28XhtF2qtb8%3D Occurrence Handle10.1007/BF00290372

    Article  PubMed  CAS  Google Scholar 

  • Ding, S., X. Wu, G. Li, M. Han, Y. Zhuang & T. Xu, 2005. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell (Aug 12) 122(3): 473–483

    Google Scholar 

  • T.A. Elick C.A. Bauser M.J. Fraser (1996) ArticleTitleExcision of the piggyBac transposable element in vitro is a precise event that is enhanced by the expression of its encoded transposase Genetica 98 33–41 Occurrence Handle8765680 Occurrence Handle1:CAS:528:DyaK28Xlslyntb8%3D Occurrence Handle10.1007/BF00120216

    Article  PubMed  CAS  Google Scholar 

  • T.A. Elick N. Lobo M.J. Fraser (1997) ArticleTitleAnalysis of the cis-acting DNA elements required for piggyBac transposable element excision Mol. Gen. Genet. 255 605–610 Occurrence Handle9323364 Occurrence Handle1:CAS:528:DyaK2sXmtl2hu70%3D Occurrence Handle10.1007/s004380050534

    Article  PubMed  CAS  Google Scholar 

  • Fraser, M.J., 2001. The TTAA-specific family of transposable elements: identification, functional characterization, and utility for transformation of insects, pp. 249–268, in Insect Transgenesis: Methods and Applications, edited by A.M. Handler & A.A. James. Boca Raton, Florida, CRC Press

  • M.J. Fraser J.S. Brusca G.E. Smith M.D. Summers (1985) ArticleTitleTransposon-mediated mutagenesis of a Baculovirus Virology 145 356–361 Occurrence Handle2992159 Occurrence Handle1:CAS:528:DyaL2MXltFOhtb8%3D Occurrence Handle10.1016/0042-6822(85)90172-2

    Article  PubMed  CAS  Google Scholar 

  • M.J. Fraser L. Cary K. Boonvisudhi H.H. Wang (1995) ArticleTitleAssay for movement of Lepidopteran transposon IFP2 in insect cells using a Baculovirus genome as a target DNA Virology 211 397–407 Occurrence Handle7645244 Occurrence Handle1:CAS:528:DyaK2MXnsFGjsr4%3D Occurrence Handle10.1006/viro.1995.1422

    Article  PubMed  CAS  Google Scholar 

  • M.J. Fraser T. Ciszczon T. Elick C. Bauser (1996) ArticleTitlePrecise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of lepidoptera Insect Mol. Biol. 5 141–151 Occurrence Handle8673264 Occurrence Handle1:CAS:528:DyaK28XhsFylsb0%3D

    PubMed  CAS  Google Scholar 

  • M.J. Fraser G.E. Smith M.D. Summers (1983) ArticleTitleAcquisition of host cell DNA sequences by baculoviruses: relationship between host DNA insertions and FP mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses J. Virol. 47 287–300 Occurrence Handle16789244 Occurrence Handle1:CAS:528:DyaL3sXltVSitLY%3D

    PubMed  CAS  Google Scholar 

  • N. Gaiano M. Allende A. Amsterdam K. Kawakami N. Hopkins (1996a) ArticleTitleHighly efficient germ-line transmission of proviral insertions in zebrafish Proc. Natl. Acad. Sci. 93 7777–7782 Occurrence Handle1:CAS:528:DyaK28XksFSrtrY%3D Occurrence Handle10.1073/pnas.93.15.7777

    Article  CAS  Google Scholar 

  • N. Gaiano A. Amsterdam K. Kawakami M. Allende T. Becker N. Hopkins (1996b) ArticleTitleInsertional mutagenesis and rapid cloning of essential genes in zebrafish Nature 383 829–832 Occurrence Handle1:CAS:528:DyaK28Xms1Sisr8%3D Occurrence Handle10.1038/383829a0

    Article  CAS  Google Scholar 

  • Y. Gluzman (1981) ArticleTitleSV40-transformed simian cells support the replication of early SV40 mutants Cell 23 175–182 Occurrence Handle6260373 Occurrence Handle1:CAS:528:DyaL3MXotFyiug%3D%3D Occurrence Handle10.1016/0092-8674(81)90282-8

    Article  PubMed  CAS  Google Scholar 

  • C. Gonzalez-Estevez T. Momose W.J. Gehring E. Salo (2003) ArticleTitleTransgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker Proc. Natl. Acad. Sci. 100 IssueID24 14046–14051 Occurrence Handle14615580 Occurrence Handle1:CAS:528:DC%2BD3sXpsFGit7w%3D Occurrence Handle10.1073/pnas.2335980100

    Article  PubMed  CAS  Google Scholar 

  • G.L. Grossman C.S. Rafferty J.R. Clayton T.K. Stevens O. Mukabayire M.Q. Benedict (2001) ArticleTitleGermline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element Insect Mol. Biol. 10 597–604 Occurrence Handle11903629 Occurrence Handle1:CAS:528:DC%2BD38Xps1Gk Occurrence Handle10.1046/j.0962-1075.2001.00299.x

    Article  PubMed  CAS  Google Scholar 

  • G.L. Grossman C.S. Rafferty M.J. Fraser M.Q. Benedict (2000) ArticleTitleThe piggyBac element is capable of precise excision and transposition in cells and embryos of the mosquito, Anopheles gambiae Insect Biochem. Mol. Biol. 30 909–914 Occurrence Handle10899457 Occurrence Handle1:CAS:528:DC%2BD3cXkvVyrsrk%3D Occurrence Handle10.1016/S0965-1748(00)00092-8

    Article  PubMed  CAS  Google Scholar 

  • U. Hacker S. Nystedt M.P. Barmchi C. Horn E.A. Wimmer (2003) ArticleTitle piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila Proc. Natl. Acad. Sci. 100 7720–7725 Occurrence Handle12802016 Occurrence Handle10.1073/pnas.1230526100 Occurrence Handle1:CAS:528:DC%2BD3sXlt1Wqs7w%3D

    Article  PubMed  CAS  Google Scholar 

  • Handler, A.M. & R.A. Harrell, 1999. Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. Insect Mol. Biol. 8: 449–457

  • A.M. Handler S. McCombs M.J. Fraser S.H. Saul (1998) ArticleTitleThe lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly Proc. Natl. Acad. Sci. 95 7520–7525 Occurrence Handle9636182 Occurrence Handle1:CAS:528:DyaK1cXktFOgsbc%3D Occurrence Handle10.1073/pnas.95.13.7520

    Article  PubMed  CAS  Google Scholar 

  • A.M. Handler G.J. Zimowski C. Horn (2004) ArticleTitlePost-Integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster Nat. Biotechnol. 22 IssueID9 1150–1154 Occurrence Handle15300258 Occurrence Handle1:CAS:528:DC%2BD2cXntFCgtbw%3D Occurrence Handle10.1038/nbt1002

    Article  PubMed  CAS  Google Scholar 

  • B. Hirt (1967) ArticleTitleSelective extraction of polyoma DNA from infected mouse cell cultures J. Mol. Biol. 26 365–369 Occurrence Handle4291934 Occurrence Handle1:CAS:528:DyaF2sXkslWktr0%3D Occurrence Handle10.1016/0022-2836(67)90307-5

    Article  PubMed  CAS  Google Scholar 

  • C. Horn N. Offen S. Nystedt U. Häcker E.A. Wimmer (2003) ArticleTitle piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics Genetics 163 647–661 Occurrence Handle12618403 Occurrence Handle1:CAS:528:DC%2BD3sXislWjtbk%3D

    PubMed  CAS  Google Scholar 

  • Z. Ivics Z. Izsvák P.B. Hackett (1997) ArticleTitleMolecular reconstruction of Sleeping Beauty, a Tcl-like transposon system from fish and its transposition in human cells Cell 91 501–510 Occurrence Handle9390559 Occurrence Handle1:CAS:528:DyaK2sXnsFGlsbk%3D Occurrence Handle10.1016/S0092-8674(00)80436-5

    Article  PubMed  CAS  Google Scholar 

  • Z. Izsvak Z. Ivics R.H. Plasterk (2000) ArticleTitle Sleeping Beauty, a wide host-range transposon vector for genetic transformation in vertebrates J. Mol. Biol. 302 93–102 Occurrence Handle10964563 Occurrence Handle1:CAS:528:DC%2BD3cXmtVGntr0%3D Occurrence Handle10.1006/jmbi.2000.4047

    Article  PubMed  CAS  Google Scholar 

  • K. Kawakami A. Koga H. Hori A. Shima (1998) ArticleTitleExcision of the Tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio Gene 225 17–22 Occurrence Handle9931412 Occurrence Handle1:CAS:528:DyaK1MXns1ensw%3D%3D Occurrence Handle10.1016/S0378-1119(98)00537-X

    Article  PubMed  CAS  Google Scholar 

  • K. Kawakami A. Shima N. Kawakami (2000) ArticleTitleIdentification of a functional transposase of the Tol2 element, and Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage PNAS 97 IssueID21 11403–11408 Occurrence Handle11027340 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2qsr4%3D Occurrence Handle10.1073/pnas.97.21.11403

    Article  PubMed  CAS  Google Scholar 

  • W. Kim K. Koo A.M. Richman D. Seely J. Vizioli A.D. Klocko D.A. O’Brochta (2004) ArticleTitleEctopic expression of a cecropin transgene in the human malaria vector mosquito Anopheles gambiae (Diptera: Culcidae): effects on susceptibility to Plasmodium J. Med. Entomol. 41 IssueID3 447–455 Occurrence Handle15185949 Occurrence Handle1:CAS:528:DC%2BD2cXkslCjsb8%3D Occurrence Handle10.1603/0022-2585-41.3.447

    Article  PubMed  CAS  Google Scholar 

  • V. Kokoza A. Ahmed E.A. Wimmer A.S. Raikhel (2001) ArticleTitleEfficient transformation of the yellow fever mosquito Aedes aegypti using the piggyBac transposable element vector pBac[3xP3-EGFP afm] Insect Biochem. Mol. Biol. 31 1137–1143 Occurrence Handle11583926 Occurrence Handle1:CAS:528:DC%2BD3MXntF2qtr0%3D Occurrence Handle10.1016/S0965-1748(01)00120-5

    Article  PubMed  CAS  Google Scholar 

  • R. Korn M. Schoor H. Neuhaus U. Henseling R. Soininen J. Zachgo A. Gossler (1992) ArticleTitleEnhancer trap integrations in mouse embryonic stem cells give rise to staining patterns in chimaeric embryos with a high frequency and detect endogenous genes Mech. Dev. 39 95–109 Occurrence Handle1283314 Occurrence Handle1:STN:280:DyaK3s7ksVWrsw%3D%3D Occurrence Handle10.1016/0925-4773(92)90029-J

    Article  PubMed  CAS  Google Scholar 

  • X. Li R.A. Harrell A.M. Handler T. Beam K. Hennessy M.J. Fraser (2005) ArticleTitle piggyBac internal sequences are necessary for efficient transformation of target genomes Insect Mol. Biol. 14 17–30 Occurrence Handle15663772 Occurrence Handle10.1111/j.1365-2583.2004.00525.x Occurrence Handle1:CAS:528:DC%2BD2MXhtlamt7c%3D

    Article  PubMed  CAS  Google Scholar 

  • S. Lin N. Gaiano P. Culp J.C. Burns T. Friedmann J.K. Yee N. Hopkins (1994) ArticleTitleIntegration and germ-line transmission of a pseudotyped retroviral vector in zebrafish Science 265 666–669 Occurrence Handle8036514 Occurrence Handle1:CAS:528:DyaK2cXlt1KgsbY%3D Occurrence Handle10.1126/science.8036514

    Article  PubMed  CAS  Google Scholar 

  • E. Linney N.L. Hardison B.E. Lonze S. Lyons L. DiNapoli (1999) ArticleTitleTransgene expression in zebrafish: a comparison of retroviral-vector and DNA-injection approaches Dev. Biol. 213 207–216 Occurrence Handle10452858 Occurrence Handle1:CAS:528:DyaK1MXlt1Wrs7s%3D Occurrence Handle10.1006/dbio.1999.9376

    Article  PubMed  CAS  Google Scholar 

  • N.F. Lobo A. Hua-Van X. Li B.M. Nolen M.J. Fraser (2002) ArticleTitleGerm line transformation of the yellow fever mosquito, Aedes aegypti, mediated by transpositional insertion of a piggyBac vector Insect Mol. Biol. 11 133–139 Occurrence Handle11966878 Occurrence Handle1:CAS:528:DC%2BD38XjvFSiu74%3D Occurrence Handle10.1046/j.1365-2583.2002.00317.x

    Article  PubMed  CAS  Google Scholar 

  • N. Lobo X. Li M.J. Fraser (1999) ArticleTitleTransposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni Mol. Gen. Genet. 261 803–810 Occurrence Handle10394918 Occurrence Handle1:CAS:528:DyaK1MXks12htr4%3D Occurrence Handle10.1007/s004380050024

    Article  PubMed  CAS  Google Scholar 

  • N. Lobo X. Li A. Hua-Van M.J. Fraser (2001) ArticleTitleMobility of the piggyBac transposon in embryos of the vectors of Dengue fever (Aedes albopictus) and La Crosse encephalitis (Ae. triseriatus) Mol. Genet. Genomics 265 66–71 Occurrence Handle11370874 Occurrence Handle1:CAS:528:DC%2BD3MXjsVegu7k%3D Occurrence Handle10.1007/s004380000388

    Article  PubMed  CAS  Google Scholar 

  • M.D. Lorenzen A.J. Berghammer S.J. Brown R.E. Denell M. Klingler R.W. Beeman (2003) ArticleTitle piggyBac-mediated germline transformation in the beetle Tribolium castaneum Insect Mol. Biol. 12 IssueID5 433–440 Occurrence Handle12974948 Occurrence Handle1:CAS:528:DC%2BD3sXotVSqsrs%3D Occurrence Handle10.1046/j.1365-2583.2003.00427.x

    Article  PubMed  CAS  Google Scholar 

  • A.L. Parks K.R. Cook M. Belvin N.A. Dompe R. Fawcett K. Huppert L.R. Tan C.G. Winter K.P. Bogart J.E. Deal M.E. Deal-Herr D. Grant M. Marcinko W.Y. Miyazaki S. Robertson K.J. Shaw M. Tabios V. Vysotskaia L. Zhao R.S. Andrade K.A. Edgar E. Howie K. Killpack B. Milash A. Norton D. Thao K. Whittaker M.A. Winner L. Friedman J. Margolis M.A. Singer C. Kopczynski D. Curtis T.C. Kaufman G.D. Plowman G. Duyk H.L. Francis-Lang (2004) ArticleTitleSystematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome Nat. Genet. 36 IssueID3 288–292 Occurrence Handle14981519 Occurrence Handle1:CAS:528:DC%2BD2cXhsFOnsL8%3D Occurrence Handle10.1038/ng1312

    Article  PubMed  CAS  Google Scholar 

  • G. Romano K. Reiss X. Tu F. Peruzzi B. Belletti J.Y. Wang T. Zanocco-Marani R. Baserga (2001) ArticleTitleEfficient in vitro and in vivo gene regulation of a retrovirally delivered pro-apoptotic factor under the control of the Drosophila HSP70 promoter Gene Ther. 8 600–607 Occurrence Handle11320406 Occurrence Handle1:CAS:528:DC%2BD3MXjtV2rtL8%3D Occurrence Handle10.1038/sj.gt.3301441

    Article  PubMed  CAS  Google Scholar 

  • E. Ryder S. Russell (2003) ArticleTitleTransposable elements as tools for genomics and genetics in Drosophila Brief Funct. Genomic Proteomic 2 57–71 Occurrence Handle15239944 Occurrence Handle1:CAS:528:DC%2BD3sXkslOjurY%3D Occurrence Handle10.1093/bfgp/2.1.57

    Article  PubMed  CAS  Google Scholar 

  • F. Sablitzky J.-I. Jonsson B.L. Cohen R.A. Phillips (1993) ArticleTitleHigh frequency expression of integrated proviruses derived from enhancer trap retroviruses Cell Growth Differ. 4 451–459 Occurrence Handle8373730 Occurrence Handle1:CAS:528:DyaK3sXmtFylsro%3D

    PubMed  CAS  Google Scholar 

  • A. Sarkar C.J. Coates S. Whyard U. Willhoeft P.W. Atkinson D.A. O’Brochta (1997) ArticleTitleThe Hermes element from Musca domestica can transpose in four families of cyclorrhaphan flies Genetica 99 15–29 Occurrence Handle9226434 Occurrence Handle1:CAS:528:DyaK2sXltlKmtbs%3D

    PubMed  CAS  Google Scholar 

  • A. Sarkar C. Sim Y.S. Hong J.R. Hogan M.J. Fraser H.M. Robertson F.H. Collins (2003) ArticleTitleMolecular evolutionary analysis of the widespread piggyBac transposon family and related “domesticated” sequences Mol. Genet. Genomics 270 173–180 Occurrence Handle12955498 Occurrence Handle1:CAS:528:DC%2BD3sXoslWkt7g%3D Occurrence Handle10.1007/s00438-003-0909-0

    Article  PubMed  CAS  Google Scholar 

  • S.T. Thibault H.T. Luu N. Vann T.A. Miller (1999) ArticleTitlePrecise excision and transposition of piggyBac in pink bollworm embryos Insect Mol. Biol. 8 119–123 Occurrence Handle9927180 Occurrence Handle1:CAS:528:DyaK1MXnsVersA%3D%3D Occurrence Handle10.1046/j.1365-2583.1999.810119.x

    Article  PubMed  CAS  Google Scholar 

  • S.T. Thibault M.A. Singer W.Y. Miyazaki B. Milash N.A. Dompe C.M. Singh R. Buchholz M. Demsky R. Fawcett H.L. Francis-Lang L. Ryner L.M. Cheung A. Chong C. Erickson W.W. Fisher K. Greer S.R. Hartouni E. Howie L. Jakkula D. Joo K. Killpack A. Laufer J. Mazzotta R.D. Smith L.M. Stevens C. Stuber L.R. Tan R. Ventura A. Woo I. Zakrajsek L. Zhao F. Chen C. Swimmer C. Kopczynski G. Duyk M.L. Winberg J. Margolis (2004) ArticleTitleA complementary transposon tool kit for Drosophila melanogaster using P and piggyBac Nat. Genet. 36 283–287 Occurrence Handle14981521 Occurrence Handle1:CAS:528:DC%2BD2cXhsFOnsL0%3D Occurrence Handle10.1038/ng1314

    Article  PubMed  CAS  Google Scholar 

  • H.H. Wang M.J. Fraser L.C. Cary (1989) ArticleTitleTransposon mutagenesis of Baculoviruses: analysis of TFP3 lepidopteran insertions at the FP locus of nuclear polyhedrosis viruses Gene 81 97–108 Occurrence Handle2553540 Occurrence Handle1:CAS:528:DyaK3cXksFehsbs%3D Occurrence Handle10.1016/0378-1119(89)90340-5

    Article  PubMed  CAS  Google Scholar 

  • Westerfield, M., 1993. The Zebrafish Book. University of Oregon Press, Eugene, OR

  • J.-W. Xiong A. Leahy H. Stuhlmann (1999) ArticleTitleRetroviral promoter-trap insertion into a novel mammalian septin gene expressed during mouse neuronal development Mech. Dev. 86 183–191 Occurrence Handle10446280 Occurrence Handle1:CAS:528:DyaK1MXlt1Sgs70%3D Occurrence Handle10.1016/S0925-4773(99)00113-6

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Center for Tropical Diseases Research and Training, University of Notre Dame, P.O. Box 369, Notre Dame, IN, 46556-0369, USA

    Neil F. Lobo, Tresa S. Fraser, John A. Adams & Malcolm J. Fraser Jr.

Authors
  1. Neil F. Lobo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tresa S. Fraser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John A. Adams
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Malcolm J. Fraser Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Malcolm J. Fraser Jr..

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lobo, N.F., Fraser, T.S., Adams, J.A. et al. Interplasmid transposition demonstrates piggyBac mobility in vertebrate species. Genetica 128, 347–357 (2006). https://doi.org/10.1007/s10709-006-7165-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-006-7165-2

Keywords

Search

Navigation