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A Comparative Cytogenetic Analysis between the Grasshopper Species Chromacris nuptialis and C. speciosa (Romaleidae): Constitutive Heterochromatin Variability and rDNA Sites

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Abstract

The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using different cytogenetic techniques, in order to determine the level of karyotypic similarities and differences between the species. The results show similarities in chromosome number (2n = 23,X0) and acrocentric morphology. In some C. nuptialis individuals meiotic irregularities were detected involving the L2 bivalent. This bivalent was delayed and presented anaphasic bridges and other aberrations. Differences in constitutive heterochromatin (CH) patterns and composition were observed through C-banding and fluorochromes staining. Silver nitrate staining revealed a single medium nucleolar organizer regions (NORs) pair, per species. Differences were also observed in NORs location, which was pericentromeric in C. nuptialis and proximal in C. speciosa. FISH using an rDNA probe confirmed the existence of ribosomal sites coinciding with active regions visualized by silver nitrate. The possible implications of the karyotype differences observed between both species are discussed.

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References

  • J.L. Bella J. Gosálvez R.A. Nichols C. López-Fernández C. García de la Vega G.M. Hewitt (1990) ArticleTitleChromosome divergence in Podisma Berthold through the Alps, Pyrenees and Sistema Ibérico Bol. San. Veg. Plagas 20 349–358

    Google Scholar 

  • J.R. Bridle J. la Torre Particlede J.L. Bella R.K. Butlin J. Gosálvez (2002) ArticleTitleLow levels of chromosomal differentiation between the grasshoppers Chorthippus brunneus and Chorthippus jacobsi (Orthoptera;Acrididae) in northern Spain Genetica 114 121–127 Occurrence Handle10.1023/A:1015185122507 Occurrence Handle12041825

    Article  PubMed  Google Scholar 

  • J.P.M. Camacho J. Cabrero (1983) ArticleTitleKaryological diferences between two species of grasshopper genus Acrotylus (Acrididae: Oedipodinae) Caryologia 36 121–127

    Google Scholar 

  • D. Cimini M. Mattiuzzo L. Torosantucci F. Degrassi (2003) ArticleTitleHistone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects Mol. Biol. Cell 14 3821–3833 Occurrence Handle10.1091/mbc.E03-01-0860 Occurrence Handle12972566

    Article  PubMed  Google Scholar 

  • J. Dubcovsky J. Dvorak (1995) ArticleTitleRibossomal RNA multigene loci: nomads of the Triticeae genomes Genetics 140 1367–1377 Occurrence Handle7498776

    PubMed  Google Scholar 

  • A. Fluminhan SuffixJr. M.L.R. Aguiar-Perecin J.A. Dos Santos (1996) ArticleTitleEvidence for heterochromatin involvement in chromosome breakage in maize callus culture Ann. Bot. 78 73–81 Occurrence Handle10.1006/anbo.1996.0098

    Article  Google Scholar 

  • B. John M. King D. Schweizer M. Mendelak (1985) ArticleTitleEquilocality of heterochromatin distribution and heterochromatin heterogeneity in acridid grasshoppers Chromosoma 91 185–200 Occurrence Handle10.1007/BF00328216 Occurrence Handle3979177

    Article  PubMed  Google Scholar 

  • K.E. Koehler E.A. Millie J.P. Cherry P.S. Burgoyne E.P. Evans P.A. Hunt T.J. Hassold (2002) ArticleTitleSex-specific differences in meiotic chromosome segregation revealed by dicentric bridge resolution in mice Genetics 162 1367–1379 Occurrence Handle12454080

    PubMed  Google Scholar 

  • R. LeMaire-Adkins K. Radke P.A. Hunt (1997) ArticleTitleLack of checkpoint control at the metaphase/anaphase transition: A mechanism of meiotic nondisjunction in mammalian females J. Cell Biol. 139 1611–1619 Occurrence Handle10.1083/jcb.139.7.1611 Occurrence Handle9412457

    Article  PubMed  Google Scholar 

  • A. Mesa A. Ferreira C.S. Carbonell (1982) ArticleTitleCariologia de los acridoideos neotropicales: estado actual de su conocimento y nuevas contribuciones Ann. Soc. Ent. Fr. (N. S.) 18 507–526

    Google Scholar 

  • E.A. Moscone M.A. Matzke A.J.M. Matzke (1996) ArticleTitleThe use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco Chromosoma 105 231–236 Occurrence Handle10.1007/s004120050179

    Article  Google Scholar 

  • A. Pedrosa M.F. Jantsch E.A. Moscone P.F. Ambros D. Schweizer (2001) ArticleTitleCharacterisation of pericentromeric and sticky intercalary heterochromatin in Ornithogalum longibracteatum (Hyacinthaceae) Chromosoma 110 203–213 Occurrence Handle11513295

    PubMed  Google Scholar 

  • L.G. Pereira M.J. Souza (2000) ArticleTitleNature and distribution of constitutive heterochromatin and NOR location in the grasshopper Phaeoparia megacephala (Romaleidae: Orthoptera) Cytobios 103 111–119 Occurrence Handle11077973

    PubMed  Google Scholar 

  • P. Rab E.J. Crossman K.M. Reed M. Rabova (2002) ArticleTitleChromosomal characteristics of ribosomal DNA in two extant species of North American mudminnows Umbra pygmaea and U. limi (Euteleostei: Umbridae) Cytogenet. Genome Res. 98 194–198 Occurrence Handle10.1159/000069800 Occurrence Handle12698003

    Article  PubMed  Google Scholar 

  • H.R. Roberts C.S. Carbonell (1982) ArticleTitleA revision of thr grasshopper genera Chromacris and Xestotrachelus (Orthoptera, Romaleidae, Romaleinae) Proc. Calif. Acad. Sci. 43 43–58

    Google Scholar 

  • M.F. Rocha M.J. Souza T. Tashiro (1997) ArticleTitleKaryotype variability in the Genus Radacridium (Orthoptera- Romaleidae) Cytologia 62 53–60

    Google Scholar 

  • E. Rodríguez-Iñigo J.L. Bella C. García de la Vega (1993) ArticleTitleHeterochromatin diferentiation between two species of the genus Dociostaurus (Orthoptera: Acrididae) Heredity 70 458–465

    Google Scholar 

  • J.S. Rufas P. Esponda J. Gosálvez (1985) ArticleTitleNOR and nucleolus in the spermatogenesis of acridoid grasshoppers Genetica 66 139–144 Occurrence Handle10.1007/BF00139718

    Article  Google Scholar 

  • D. schweizer M. Mendelak M.J.D. White N. Contreras (1983) ArticleTitleCytogenetics of the parthenogenetic grasshopper Warramaba virgo and its bisexual relatives. X. Pattern of fluorescent banding Chromosoma 88 227–236 Occurrence Handle10.1007/BF00285625

    Article  Google Scholar 

  • M.J. Souza M.H.N. Silva-Filha (1993) ArticleTitleEffects of extra chromosome segments on chiasma distribution in Xyleus angulatus (Orthoptera, Romaleidae) Rev. Brasil. Genet. 16 23–33

    Google Scholar 

  • M.J. Souza L.M.H. Kido (1995) ArticleTitleVariability of constitutive heterochromatin in karyotypes of representatives of the family Romaleidae (Orthoptera) Rev. Brasil. Genet. 18 517–520

    Google Scholar 

  • M.J. Souza J.S. Rufas J. Orellana (1998) ArticleTitleConstitutive heterochromatin, NOR location and FISH in the grasshopper Xyleus angulatus (Romaleidae) Caryologia 51 73–80

    Google Scholar 

  • M.J. Souza P.R.O. Haver N.F. Melo (2003) ArticleTitleKaryotype, C- and fluorescence banding patterns, NOR location and FISH in the grasshopper Xestotrachelus robustus (Romaleidae) Caryologia 56 261–267

    Google Scholar 

  • A.T. Sumner (1972) ArticleTitleA simple technique for demonstrating centromeric heterochromatin Exp. Cell Res. 75 304–306 Occurrence Handle10.1016/0014-4827(72)90558-7 Occurrence Handle4117921

    Article  PubMed  Google Scholar 

  • I. Unfried U. Stocker P. Gruendler (1989) ArticleTitleNucleotide sequence of the 18S rRNA genes from Arabidopsis thaliana Nucleic Acids Res. 17 7513 Occurrence Handle2798101

    PubMed  Google Scholar 

  • I. Unfried P. Gruendler (1990) ArticleTitleNucleotide sequence 5.8S and 25S rRNA genes and of the internal spacers from Arabidopsis thaliana Nucleic Acids Res. 18 4011 Occurrence Handle2100998

    PubMed  Google Scholar 

  • J.C. Vilardi (1986) ArticleTitleParallel polymorphism for intersticial C-bands and B-chromosomes in Zoniopoda tarsata (Orthoptera-Romaleidae) Caryologia 39 365–380

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Genética, CCB, Universidade Federal de Pernambuco, Brasil

    Vilma Loreto, Eva Stadtler & Maria José de Souza

  2. Embrapa Semi-árido, Petrolina, Brasil

    Natoniel F. de Melo

  3. Av. Prof. Moraes Rego s/n Cidade, Universitária, Recife, PE, 50732-970, Brasil

    Vilma Loreto

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  1. Vilma Loreto
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  2. Eva Stadtler
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  3. Natoniel F. de Melo
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  4. Maria José de Souza
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Correspondence to Vilma Loreto.

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Loreto, V., Stadtler, E., de Melo, N.F. et al. A Comparative Cytogenetic Analysis between the Grasshopper Species Chromacris nuptialis and C. speciosa (Romaleidae): Constitutive Heterochromatin Variability and rDNA Sites. Genetica 125, 253–260 (2005). https://doi.org/10.1007/s10709-005-0368-0

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  • DOI: https://doi.org/10.1007/s10709-005-0368-0

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