Abstract
Sexually mature males (BW = 1600 ± 150 g and TL = 235 ± 30 mm) of northern pike (Esox lucius L.) were randomly selected from a pond to record changes in their sperm quality parameters (spermatozoa morphology, sperm volume, density, and motility parameters) during the spawning season. The morphological and motility parameters changed significantly during the reproductive season with following trends. Only, head width was not changed during the spawning season. The longest spermatozoa and its flagellar length were found at the middle of spawning period (TL = 38.24 ± 0.37 μm and 35.14 ± 0.26 μm) and shortest at the beginning of spawning period (TL = 34.81 ± 0.29 μm and 32.53 ± 0.18 μm). Other morphological characters were always the lowest at the beginning of spawning period. Sperm volume was changed from 0.33 ± 0.3 ml in February, 0.43 ± 0.2 ml in March to 0.24 ± 0.1 ml in April, and density from 16.2 ± 0.2 × 109 spermatozoa ml−1 in February, 19.4 ± 0.2 × 109 spermatozoa ml−1 in March to 4.8 ± 0.2 × 109 spermatozoa ml−1 in April. Same sperm velocity was observed in all spawning terms at 10 and 20 s after activation. Higher velocity was found at 30 and 40 s after activation in sperm collected at the middle and the end of spawning period. Significantly, higher percentage of motile sperm was observed at 20, 30, and 40 s after activation in sperm sampled at the end of spawning period. This study supports the hypothesis that longer spermatozoa swim faster.
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References
Alavi SMH, Cosson J (2006) Sperm motility in fishes: (II) effects of ions and osmolality. Cell Biol Int 30:1–14. https://doi.org/10.1016/j.cellbi.2005.06.004
Alavi SMH, Psenicka M, Rodina M et al (2008) Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus (Teleostei: Cyprinidae) during the reproductive season. Aquat Living Resour 21:75–80. https://doi.org/10.1051/alr:2008011
Alavi SMH, Rodina M, Viveiros A et al (2009) Effects of osmolality on sperm morphology, motility and flagellar wave parameters in northern pike (Esox lucius L.). Theriogenology 72:32–43. https://doi.org/10.1016/j.theriogenology.2009.01.015
Alavi SMH, Rodina M, Hatef A, Stejskal V, Policar T, Hamáčková J, Linhart O (2010) Sperm motility and monthly variations of semen characteristics in Perca fluviatilis (Teleostei: Percidae). Czech J Anim Sci 55:174–182
Babiak I, Ottesen O, Rudolfsen G, Johnsen S (2006) Quantitative characteristics of Atlantic halibut, Hippoglossus hippoglossus L., semen throughout the reproductive season. Theriogenology 65:1587–1604. https://doi.org/10.1016/j.theriogenology.2005.09.004
Billard R, Cosson J, Perchec G, Linhart O (1995) Biology of sperm and artificial reproduction in carp. Aquaculture 124:95–112. https://doi.org/10.1016/0044-8486(94)00231-C
Blecha M, Kristan J, Samarin AM, Rodina M, Policar T (2015) Quality and quantity of pikeperch (Sander lucioperca) spermatozoa after varying cold water treatments. J Appl Ichthyol 31(2):75–78. https://doi.org/10.1111/jai.12853
Blecha M, Samarin AM, Kristan J, Policar T (2016) Benefits of hormone treatment of both sexes in semi-artificial reproduction of pikeperch (Sander lucioperca L.). Czech J Anim Sci 61:203–208. https://doi.org/10.17221/60/2015-CJAS
Bobe J, Labbe C (2009) Chilled storage of sperm and eggs. In: Cabrita E, Robles V, Herraez P (eds) Methods in reproductive aquaculture: marine and freshwater species. Marine Biology Series. CRC Press Taylor & Francis Group, Boca Raton, pp 219–235. https://doi.org/10.1201/9780849380549.sec4
Bondarenko V, Podhorec P, Svinger V, Policar T (2015a) Evaluation of different treatments for induction of ovulation in northern pike (Esox lucius L.). Turk J Fish Aquat Sci 15:581–587. https://doi.org/10.4194/1303-2712-v15_2_46
Bondarenko V, Drozd B, Policar T (2015b) Effect of water temperature on egg incubation time and quality of newly hatched larvae of northern pike (Esox lucius L., 1758). J Appl Ichthyol 31(2):45–50. https://doi.org/10.1111/jai.12851
Boryshpolets S, Dzyuba B, Drokin S (2009) Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps. Fish Physiol Biochem 35(4):661–668. https://doi.org/10.1007/s10695-008-9292-4
Buyukhatipoglu S, Holtz W (1984) Sperm output in rainbow trout (Salmo gairdneri). Effect of age, timing, and frequency of stripping and presence of females. Aquaculture 37:63–71. https://doi.org/10.1016/0044-8486(84)90044-9
Caille N, Rodina M, Kocour M, Gela D, Flajšhans M, Linhart O (2006) Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments. Aquacult Int 14:75–87. https://doi.org/10.1007/s10499-005-9015-0
Gage MJG, MacFarlane CP, Yeates S et al (2004) Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success. Curr Biol 14:44–47. https://doi.org/10.1016/j.cub.2003.12.028
Hulak M, Rodina M, Linhart O (2008) Characteristics of stripped and testicular northern pike (Esox lucius L.) sperm: spermatozoa motility and velocity. Aquat Living Resour 21:207–212. https://doi.org/10.1051/alr:2008022
Kara MH, Labed S (1994) Évolution des caractéristiques du sperme de Sparus aurata et Dicentrarchus labrax au cours d’une saison de reproduction. Cah Biol Mar 35:281–288
Koldras M, Loir M, Maisse G, Le Gac F (1996) Study of the composition of seminal fluid and of sperm motility along the genital tract, during a spawning season in the rainbow trout (Oncorhynchus mykiss). Aquat Living Resour 9:337–345. https://doi.org/10.1051/alr:1996036
Kucska B, Müller T, Sari J et al (2005) Successful growth of the pike fingerlings (Esox lucius L.) on pellet at artificial condition. Aquaculture 246:227–230. https://doi.org/10.1016/j.aquaculture.2005.01.013
Linhart O, Mims SD, Gomelsky B et al (2000) Spermiation of paddlefish (Polyodon spathula) stimulated with injection of LHRH analogue and carp pituitary extract. Aquat Living Resour 13:1–6
Linhart O, Rodina M, Gela D, Kocour M (2004) Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture 235:619–632. https://doi.org/10.1016/j.aquaculture.2003.11.031
Munkittrick KR, Moccia RD (1987) Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constitutions. Aquaculture 64:147–156. https://doi.org/10.1016/0044-8486(87)90350-4
Piironen J (1985) Variation in the properties of milt from the Finnish landlocked salmon (Salmo salar m. sebago Girard) during a spawning season. Aquaculture 48:337–350. https://doi.org/10.1016/0044-8486(85)90136-X
Policar T, Podhorec P, Stejskal V, Hamackova J, Alavi SMH (2010) Fertilization and hatching rates and larval performance in captive common barbel (Barbus barbus L.) throughout the spawning season. J Appl Ichthyol 26:812–815. https://doi.org/10.1111/j.1439-0426.2010.01564.x
Psenicka M, Rodina M, Nebesarova J, Linhart O (2006) Ultrastructure of spermatozoa of tench Tinca tinca observation with scanning and transmission electron microscopy. Theriogenology 64:1355–1363. https://doi.org/10.1016/j.theriogenology.2006.04.040
Rideout RM, Trippel EA, Litvak MK (2004) Relationship between sperm density, spermatocrit, sperm motility and spawning date in wild and cultured haddock. J Fish Biol 65:319–332. https://doi.org/10.1111/j.0022-1112.2004.00451.x
Rodina M, Policar T, Linhart O, Rougeot C (2008) Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis). J Appl Ichthyol 24:438–442. https://doi.org/10.1111/j.1439-0426.2008.01137.x
Samarin AM, Policar T, Lahnsteiner F (2015a) Fish oocyte ageing and its effect on egg quality. Rev Fish Sci Aquac 23:302–314. https://doi.org/10.1080/23308249.2015.1053560
Samarin AM, Gela D, Bytyutskyy D, Policar T (2015b) Determination of the best post-ovulatory stripping time for the common carp, Cyprinus carpio. J Appl Ichthyol 31(2):51–55. https://doi.org/10.1111/jai.12855
Samarin AM, Blecha M, Bytyutskyy D, Policar T (2015c) Post-ovulatory oocyte ageing in pikeperch (Sander lucioperca L.) and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies. Turk J Fish Aquat Sci 15:435–441. https://doi.org/10.4194/1303-2712-v15_2_29
Samarin AM, Blecha M, Uzhytchak M, Bytyutskyy D, Zarski D, Flajshans M, Policar T (2016) Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies. Aquaculture 450:431–438. https://doi.org/10.1016/j.aquaculture.2015.08.017
Shangguan B, Crim LW (1999) Seasonal variation in sperm production and sperm quality in male winter founder, Pleuronectes americanus: the effects of hypophysectomy, pituitary replacement therapy, and GnRH-a treatment. Mar Biol 134:19–27. https://doi.org/10.1007/s002270050521
Suquet M, Dreanno C, Dorange G, Normant Y, Quemener L, Gaignon JL, Billard R (1998) The ageing phenomenon of turbot, Scophthalmus maximus, spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization and storage capacities. J Fish Biol 32:31–41. https://doi.org/10.1111/j.1095-8649.1998.tb01550.x
Westers H, Stickney RR (1993) Northern pike and muskellunge. In: Stickney R (ed) Culture of nonsalmonid freshwater fishes, 2nd edn. CRC Press, Boca Raton, FL, pp 199–213
Zuromska H (1981) Effect of different thermal regimes on reproductive cycles of tench (Tinca tinca). Part VI. Estimation of milt quality. Pol Arch Hydrobiol 28:229–241
Funding
The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic-projects “CENAKVA” (no. CZ.1.05/2.1.00/01.0024), “CENAKVA II” (no. LO1205 under the NPU I program), “Reproductive and genetic procedures for preserving fish biodiversity and aquaculture” (no. CZ.02.1.01./0.0/0.0/16_025/0007370) and following project NAZV QK1710310.
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Bondarenko, V., Blecha, M. & Policar, T. Changes of sperm morphology, volume, density, and motility parameters in northern pike during the spawning period. Fish Physiol Biochem 44, 1591–1597 (2018). https://doi.org/10.1007/s10695-018-0546-5
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DOI: https://doi.org/10.1007/s10695-018-0546-5