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Increased water temperature altered hemato-biochemical parameters and structure of peripheral erythrocytes in striped catfish Pangasianodon hypophthalmus

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Abstract

The increase of water temperature due to global warming is a great concern of aquaculturists and fishery biologists. In the present study, we examined the effects of high temperature on hematological parameters and blood glucose levels in striped catfish, Pangasianodon hypophthalmus exposed to three temperature conditions (28, 32, and 36 °C) for 7 days. Fish were sacrificed at days 1, 3, and 7. Erythroblasts (Ebs), erythrocytic cellular abnormalities (ECA), and erythrocytic nuclear abnormalities (ENA) were assayed using peripheral erythrocytes of the sampled fishes. Hemoglobin (Hb) and red blood cell (RBC) significantly (P < 0.05) decreased at 36 °C after 3 and 7 days of exposure, whereas white blood cell (WBC) showed opposite scenario. Blood glucose levels significantly (P < 0.05) increased at 36 °C on day 3. Frequencies of Ebs, ECA, and ENA were found to be elevated at increased temperature. Differential leucocytes count showed significant increases in neutrophil and decreases in lymphocytes in the highest temperature (36 °C). Dissolved oxygen decreased and free CO2 increased significantly (P < 0.05) with increasing temperature, while the pH and total alkalinity of the water were almost unchanged throughout the study period. Therefore, the present study demonstrated that striped catfish feel better adaptation at 28 and 32 °C, while high temperature 36 °C is likely stressful to this fish species.

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Acknowledgments

This study was supported by a grant (2017/282/BAU) from Bangladesh Agricultural University Research System to the corresponding author which is gratefully acknowledged.

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  1. Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Md. Shahjahan, Md. Helal Uddin, Victor Bain & Md. Mahfuzul Haque

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  1. Md. Shahjahan
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  2. Md. Helal Uddin
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  3. Victor Bain
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  4. Md. Mahfuzul Haque
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Correspondence to Md. Shahjahan.

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Shahjahan, M., Uddin, M.H., Bain, V. et al. Increased water temperature altered hemato-biochemical parameters and structure of peripheral erythrocytes in striped catfish Pangasianodon hypophthalmus. Fish Physiol Biochem 44, 1309–1318 (2018). https://doi.org/10.1007/s10695-018-0522-0

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