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Inhibition of BMP signaling reduces MMP-2 and MMP-9 expression and obstructs wound healing in regenerating fin of teleost fish Poecilia latipinna

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Abstract

The tail fin of teleost fish responds to amputation by expressing few putative factors that promote scar-free wound healing, which paves the way for restoration of the lost part. Among the factors playing a role in this initial response, bone morphogenetic proteins (BMPs) are crucial. In the current study, we have analyzed the effect of BMP inhibition on wound healing in sailfin molly Poecilia latipinna. The study involved histological assessment of wound epithelium formation, an expression profile of proteins, and gelatinase activity as well as expression in response to BMP signal inhibition. LDN193189, a pharmacological inhibitor of BMP receptor, was administered to experimental fish. Our observations include incomplete wound healing and a significant reduction in the expression of a number of proteins as a result of LDN treatment at 24 h post-amputation. A pronounced effect was also seen on the gelatinases MMP-9 and MMP-2, which showed significantly reduced activities on a zymogram. Reduced expression of these MMPs after inhibitor treatment was also confirmed by western blot and real-time PCR analyses. In view of these results, we confirm that BMP signaling has a definitive role in the early stages of fin regeneration in P. latipinna. The effect of BMP inhibition is especially seen on the expression of MMP-9 and MMP-2, which are very important effectors of tissue remodeling immediately following amputation.

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Acknowledgments

The authors are thankful to DST, New Delhi, and GSBTM, Gandhinagar, for financial support. SR, HM, PB and SP are grateful to the UGC-RGNF, DBT-MSUB-ILSPARE, CSIR and UGC-RFSMS, respectively, for research fellowships. The authors thank the anonymous reviewers of this manuscript for their constructive suggestions.

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Authors and Affiliations

  1. Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara, Gujarat, India

    Shailja Rajaram, Hiral Murawala, Pranav Buch, Sonam Patel & Suresh Balakrishnan

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  1. Shailja Rajaram
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  2. Hiral Murawala
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  3. Pranav Buch
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  4. Sonam Patel
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  5. Suresh Balakrishnan
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Correspondence to Suresh Balakrishnan.

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Rajaram, S., Murawala, H., Buch, P. et al. Inhibition of BMP signaling reduces MMP-2 and MMP-9 expression and obstructs wound healing in regenerating fin of teleost fish Poecilia latipinna . Fish Physiol Biochem 42, 787–794 (2016). https://doi.org/10.1007/s10695-015-0175-1

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