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Stocking density and Piscirickettsia salmonis infection effect on Patagonian blennie (Eleginops maclovinus, Cuvier 1830) skeletal muscle intermediate metabolism

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Abstract

The need to expand aquaculture production has led to other fish to be considered as potential species for culture, such as the sub-Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1830). The aim of this study was to determine the cumulative effect of density and pathogen infection by protein extract of Piscirickettsia salmonis on skeletal muscle metabolism. In a first experiment, specimens were submitted to three different stocking densities: (1) 3.1 kg m−3, (2) 15 kg m−3 and (3) 60 kg m−3, for a period of 10 days. In a second experiment, metabolic changes caused by an infection of P. salmonis protein extract (a single injection of 0.5 μL P. salmonis protein extract g body weight−1 was inoculated in the fish) and its combined effect with stocking density was assessed during a period of 10 days. This study concludes that stress caused by high stocking density led to the reorganization of some metabolic routes to fulfill skeletal muscle energy needs. Furthermore, infection response by pathogen P. salmonis differed when stocking density increased, suggesting an increase of energy needs with density in skeletal muscle of infected fish.

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Abbreviations

GPT:

Alanine aminotransferase (EC 2.6.1.2)

GOT:

Aspartate aminotransferase (EC 2.6.1.1)

EDTA:

Ethylenediamine tetraacetic acid

GDH:

Glutamate dehydrogenase (EC 1.4.1.2)

G3PDH:

Glycerol 3-phosphate dehydrogenase (EC 1.1.1.8)

LDH-O:

Lactate dehydrogenase oxidase (EC 1.1.1.27)

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Acknowledgments

This study was carried out in the framework of FONDECYT Projects 11080168 and 1110235 and FONDAP-INCAR, No. 15110027. We thank Dr. Lafayette Eaton for his help checking this manuscript and the Dirección de Investigación of the Universidad Austral de Chile (DID).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile

    L. Vargas-Chacoff, E. Ortíz, R. Oyarzún, D. Martínez, E. Saavedra & C. Bertrán

  2. Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna, 4860, Macul, Santiago de Chile, Chile

    R. Sá

  3. Instituto de Bioquímica y Microbiología, Interdisciplinary Center for Aquaculture Research (FONDAP-INCAR), Universidad Austral de Chile, Valdivia, Chile

    V. Olavarría, D. Nualart & A. Yáñez

  4. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI MAR), Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain

    J. M. Mancera

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  1. L. Vargas-Chacoff
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  2. E. Ortíz
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  3. R. Oyarzún
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  4. D. Martínez
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  5. E. Saavedra
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  6. R. Sá
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  7. V. Olavarría
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  8. D. Nualart
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  9. A. Yáñez
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  10. C. Bertrán
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  11. J. M. Mancera
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Correspondence to L. Vargas-Chacoff.

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Vargas-Chacoff, L., Ortíz, E., Oyarzún, R. et al. Stocking density and Piscirickettsia salmonis infection effect on Patagonian blennie (Eleginops maclovinus, Cuvier 1830) skeletal muscle intermediate metabolism. Fish Physiol Biochem 40, 1683–1691 (2014). https://doi.org/10.1007/s10695-014-9959-y

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