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Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic heat stress

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Abstract

This study investigated the effects of long-term heat exposure on Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus ♀ × summer flounder Paralichthys dentatus ♂). From 24 ± 0.5 °C, temperature was increased by 1 ± 0.5 °C in a day and was kept at that temperature for 5 days before next rise. Cumulative survival rate (CSR), cumulative survival rate under different temperature (CSR-T), histological alteration, and related enzyme activities were investigated. In P. olivaceus, mass mortality occurred at 29 and 32 °C (the CSR-T dropped to 42.39 %), and serious gill damages appeared at 30 and 32 °C. Meanwhile, the activities of superoxide dismutase (SOD), catalase (CAT), lysozyme (LZM), and pyruvate kinase (PK) declined around 29 and 32 °C (except for CAT). In comparison with P. olivaceus, the CSR of the hybrids was higher, the gill kept a better structural integrity, and the activities of SOD, CAT, LZM, and PK showed tiny fluctuations. The results suggested that during the process of chronic heat stress, P. olivaceus seemed to be more sensitive to 29 and 32 °C, and the manifestations in survival, histology, and enzyme activity were generally consistent. For the hybrids, the comparatively insensitivity to high temperature might imply its better heat tolerance.

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Acknowledgments

The authors are grateful to Professor Xiao Liu and Professor Ying Liu in Institute of Oceanology, Chinese Academy of Sciences, for kindly providing circulating water systems. This research was supported by Modern Agro-industry Technology Research System (nycytx-50), the Innovation Key Program of the Chinese Academy of Sciences and the Experiment and Demonstration of Scientific and Technical Innovation on Modern Ecological Ocean Agriculture (KSC2-EW-B-3), Science-Technology R&D project of Shandong Province (2011GHy11530), Jiangsu Provincial Natural Science Foundation of China (BK2012222), and Fundamental Research Project of Technology Program of Qingdao, China (12-1-4-8(6)-jch, 12-1-4-8-(7)-jch, and 12-4-1-51-hy).

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  1. Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Yifan Liu, Daoyuan Ma, Chunyan Zhao, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Qinghua Liu & Jun Li

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Yifan Liu & Chunyan Zhao

  3. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Xiao Liu & Ying Liu

  4. College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China

    Wenqi Wang & Xuelei Zhang

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  1. Yifan Liu
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Liu, Y., Ma, D., Zhao, C. et al. Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic heat stress. Fish Physiol Biochem 40, 1031–1041 (2014). https://doi.org/10.1007/s10695-013-9903-6

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