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Effects of sex and season in haematological parameters and cellular composition of spleen and head kidney of pejerrey (Odontesthes bonariensis)

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Abstract

Phylogenetic diversity in fish determines high interspecific variability in morphology as well as in physiological parameters. Moreover, several haematological variables and the organ composition of haemolymphopoietic sites may vary according to sex or season. The aim of this study was to establish the haematological parameters and the cellular composition of haemolymphopoietic organs in Odontesthes bonariensis, a commercially valuable fish species in Argentina, and also to determine gender or seasonal variations. Haematocrit exhibited the highest value in summer, while haemoglobin concentration was greater in summer and autumn. Erythrocyte count was higher in spring than autumn and winter, but did not differ with summer. The increase in these variables in seasons with higher water temperatures might be a compensatory mechanism to compensate the lower level of oxygen in the environment. Leucocyte formula and blast haemolymphopoietic cells in spleen and head kidney also showed annual variations since cells related to specific immune response, i.e., lymphocytes and thrombocytes, decrease in winter, whereas cells of the non-specific immune pathways, such as granulocyte, rise. The elevation of a particular type of circulating leucocyte was preceded by an increase in values of its precursor in blood in the previous season. Both, spleen and head kidney were active in haemolymphopoiesis, although with some differences in their activity during different seasons. Males showed higher values of circulating lymphoblasts and granulocytes than females, whereas females exhibited higher values of thrombocytes. This study corroborates the high interspecific variations in haematological parameters in fish that underlines the needing of basic studies in order to assess fish health status in new promising species for aquaculture.

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Acknowledgments

The authors thank Enrique Grilli from Aguas Santafesinas S.A. for water analyses. This work was partially supported by a grant of the Agencia Nacional de Promoción Científica y Tecnológica Argentina (project PICT 2008–1263) given to F. A. Vigliano.

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Authors and Affiliations

  1. Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170, Casilda, Argentina

    Fabricio A. Vigliano, Adolfo M. Araujo, Andrés J. Marcaccini, María V. Marengo & Eliana Cattaneo

  2. Centro de Investigaciones en Piscicultura Experimental, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170, Casilda, Argentina

    Fabricio A. Vigliano & Andrés J. Marcaccini

  3. Cátedra de Semiología y Análisis Clínicos, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170, Casilda, Argentina

    Carina Peirone & Lucrecia G. M. Dasso

  4. Centro Científico Tecnológico Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rosario, Argentina

    Fabricio A. Vigliano

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  1. Fabricio A. Vigliano
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  2. Adolfo M. Araujo
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  3. Andrés J. Marcaccini
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  4. María V. Marengo
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  5. Eliana Cattaneo
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  7. Lucrecia G. M. Dasso
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Correspondence to Fabricio A. Vigliano.

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Vigliano, F.A., Araujo, A.M., Marcaccini, A.J. et al. Effects of sex and season in haematological parameters and cellular composition of spleen and head kidney of pejerrey (Odontesthes bonariensis). Fish Physiol Biochem 40, 417–426 (2014). https://doi.org/10.1007/s10695-013-9853-z

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