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Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen

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Abstract

This study investigated the efficacy of the essential oil (EO) of Aloysia triphylla as an anesthetic for albino and gray strains of silver catfish, Rhamdia quelen. Juveniles were exposed to concentrations between 20 and 800 μL L−1 EO of A. triphylla to evaluate time of induction and recovery from anesthesia. In another experiment, both strains were divided into four groups such as 0 (control), 30, 40, or 50 μL L−1 EO and transported for 5 h. The longest time for anesthetic induction and recovery was observed in the albinos. Both strains reached anesthesia in the 100–800 μL L−1 (11.1–1.24 min) range, without mortality, being 200 μL L−1 the best response considering time to anesthesia (5.35 min). Albinos transported with all EO concentrations showed higher values of carbon dioxide in the water of transport, but lower levels were observed in grays transported with 40 and 50 μL L−1 EO when compared to control fish. The same concentrations did not prevent significant whole-body cortisol rise at the end of transport in the albino strain. Juveniles of both strains transported with EO presented lower ion loss to the water compared to control fish. The EO of A. triphylla is an effective anesthetic for albino and gray silver catfish. This EO increases whole-body cortisol levels in the albino strain, but as it reduces net ion loss as in the gray strain, it can be also recommended for transport.

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Acknowledgments

This study was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, process 470964/2009-0 and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul- FAPERGS- PRONEX process 10/0016-8). B. Baldisserotto received a CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) PhD fellowship.

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Authors and Affiliations

  1. Programa de Pós Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Thaylise V. Parodi & Gessi Koakoski

  2. Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Mauro A. Cunha, Alexssandro G. Becker, Carla C. Zeppenfeld, Dirlaine I. Martins & Bernardo Baldisserotto

  3. Curso de Medicina Veterinária, Universidade de Passo Fundo, Campus Universitário do Bairro São José, Passo Fundo, RS, 99001-970, Brazil

    Leonardo Gil Barcellos

  4. Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Berta M. Heinzmann

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  1. Thaylise V. Parodi
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  2. Mauro A. Cunha
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Correspondence to Bernardo Baldisserotto.

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Parodi, T.V., Cunha, M.A., Becker, A.G. et al. Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen . Fish Physiol Biochem 40, 323–334 (2014). https://doi.org/10.1007/s10695-013-9845-z

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