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Dietary probiotic supplementation (Shewanella putrefaciens Pdp11) modulates gut microbiota and promotes growth and condition in Senegalese sole larviculture

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Abstract

Probiotic supplementation in fish aquaculture has significantly increased in the last decade due to its beneficial effect on fish performance. Probiotic use at early stages of fish development may contribute to better face metamorphosis and weaning stress. In the present work, we studied the influence of Shewanella putrefaciens Pdp11 supplementation on growth, body composition and gut microbiota in Senegalese sole (Solea senegalensis) during larval and weaning development. S. putrefaciens Pdp11 was incorporated using Artemia as live vector (2.5 × 107 cfu mL−1) and supplied to sole specimens in a co-feeding regime (10–86 DAH) by triplicate. Probiotic addition promoted early metamorphosis and a significantly higher growth in length at 24 DAH larvae. S. putrefaciens Pdp11 also modulated gut microbiota and significantly increased protein content and DHA/EPA ratios in sole fry (90 DAH). This nutritional enhancement is considered especially important after weaning, where significantly higher growth in length and weight was observed in probiotic fish. Moreover, a less heterogeneous fish size in length was detected since metamorphosis till the end of weaning, being of interest for sole aquaculture production. After weaning, fish showed significantly higher growth (length and weight) and less variable lengths in fish when supplemented with probiotics. Both the enhancement of nutritional condition and the decrease in size variability associated with probiotic addition are highly interesting for sole aquaculture production.

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Acknowledgments

This study was funded by Spanish National Development Plan for Sole farming (JACUMAR) and the Ministry of Science and Technology (AGL2008-05119-C02-02 and AGL 2011-30381-C03-02). The authors wish to acknowledge the assistance of the Spanish Oceanography Institute staff and especially that of Cristina Rodríguez. We are also grateful to the EULEN/FERROSER employees and especially to Javier Revilla for their assistance. Covadonga Rodríguez belongs to the Institute of Biomedical Technologies (ITB) of the Canary Islands.

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Authors and Affiliations

  1. Spanish Institute of Oceanography, Oceanographic Center of Santander, Promontorio San Martín s/n. Apdo. 240, 39080, Santander, Spain

    Carmen Lobo & Inés García de La Banda

  2. Ecological Area of Water and Environmental Sciences and Technics, University of Cantabria, 39005, Santander, Spain

    Xabier Moreno-Ventas

  3. Department of Microbiology, Faculty of Sciences, University of Málaga, 29071, Málaga, Spain

    Silvana Tapia-Paniagua & Miguel A. Moriñigo

  4. Department of Animal Biology, Faculty of Biology, University of La Laguna, 38206, La Laguna, Spain

    Covadonga Rodríguez

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  1. Carmen Lobo
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  2. Xabier Moreno-Ventas
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  3. Silvana Tapia-Paniagua
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  4. Covadonga Rodríguez
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  5. Miguel A. Moriñigo
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  6. Inés García de La Banda
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Correspondence to Carmen Lobo.

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Lobo, C., Moreno-Ventas, X., Tapia-Paniagua, S. et al. Dietary probiotic supplementation (Shewanella putrefaciens Pdp11) modulates gut microbiota and promotes growth and condition in Senegalese sole larviculture. Fish Physiol Biochem 40, 295–309 (2014). https://doi.org/10.1007/s10695-013-9844-0

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