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The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose

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Abstract

A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets, haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and 3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles could be affected by prebiotics, which should be taken into account in future studies.

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Acknowledgments

The authors wish to thank Dr. Mohseni, Dr. Yelghi, Dr. Jafari Shamoshaki and S.N. Hosseinifar for their assistance, Dr. Van Loo and Orafti Company manager for supplying prebiotic and also the staff of Shahid Marjani and Gharesoo fish propagation and cultivation center for their kind help during the experiment.

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Authors and Affiliations

  1. Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, 31585-4314, Karaj, Iran

    Seyed Hossein Hoseinifar, Alireza Mirvaghefi & Bagher Mojazi Amiri

  2. Aquaculture and Fish Nutrition Research Group, The School of Biomedical and Biological Sciences, The University of Plymouth, Plymouth, UK

    Daniel L. Merrifield

  3. Iranian Fisheries Research Organization (IFRO), Inland Waters Fisheries Research of Gorgan, Tehran, Iran

    Saeed Yelghi

  4. Iranian National Institute for Oceanography, 14118-13389, Tehran, Iran

    Kazem Darvish Bastami

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  1. Seyed Hossein Hoseinifar
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  2. Alireza Mirvaghefi
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  3. Daniel L. Merrifield
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  4. Bagher Mojazi Amiri
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  5. Saeed Yelghi
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  6. Kazem Darvish Bastami
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Correspondence to Seyed Hossein Hoseinifar.

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Hoseinifar, S.H., Mirvaghefi, A., Merrifield, D.L. et al. The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose. Fish Physiol Biochem 37, 91–96 (2011). https://doi.org/10.1007/s10695-010-9420-9

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