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Species differences and effects of soft coral extracts from Sinnularia maximus on the expression of cytochrome P4501A and 2N in butterflyfishes (Chaetodon spp.)

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Abstract

Cytochrome P450 (CYP) has been shown to confer resistance in numerous terrestrial insects that consume potentially toxic secondary metabolites in plants, but fewer studies have examined the role of critical biotransformation enzymes in allowing marine organisms to consume chemically defended foods. This study examined the expression of CYP1A and CYP2N mRNAs in several butterflyfish species, which can feed on numerous chemically defended soft and hard corals. In addition, the effect of an extract from a soft coral (Sinnularia maxima) on expression of hepatic CYP1A and CYP2 mRNAs was also examined. Fish were fed extracts on days 1, 3 and 5, and expression was examined on day 5. Phylogenetic analyses of the CYP1A cDNA from 12 species of butterflyfish (DNA, amino acid) indicate well-separated groupings according to their feeding strategies. The non-coralline feeding Chaetodon xanthurus exhibited a 7-fold higher basal expression of CYP2N8 relative to the other species studied. Although induction of CYP2N7 expression was observed in C. punctatofasciatus, CYP1A and CYP2N was largely unaffected or diminished by extract treatment in the other species of butterflyfish. These results indicated groupings of feeding strategy with CYP1A phylogeny in Chaetodon, but generally unaltered expression of CYP1A and CYP2N following dietary treatment with an extract from a chemically defended soft coral suggesting an inconclusive role of these isoforms in the detoxification of chemicals in these extracts.

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Acknowledgements

The authors wish to thank Rebecca Polcyn for her help in fish collection and the staff at Lizard Island Research Station on the Great Barrier Reef in Australia for their assistance with collections of butterflyfish.

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Authors and Affiliations

  1. Department of Biology, Ouachita Baptist University, Arkadelphia, AR, 71998, USA

    B. C. DeBusk

  2. Department of Pharmacology, School of Pharmacy, University of Mississippi, University, MS, 38677, USA

    M. Slattery

  3. Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea

    Jang-Seu Ki & Jae-Seong Lee

  4. Department of Environmental Sciences, University of California, Riverside, Riverside, CA, 92521, USA

    Rosaura Aparicio-Fabre & Daniel Schlenk

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  1. B. C. DeBusk
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  2. M. Slattery
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  3. Jang-Seu Ki
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  4. Jae-Seong Lee
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  5. Rosaura Aparicio-Fabre
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  6. Daniel Schlenk
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Correspondence to Daniel Schlenk.

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DeBusk, B.C., Slattery, M., Ki, JS. et al. Species differences and effects of soft coral extracts from Sinnularia maximus on the expression of cytochrome P4501A and 2N in butterflyfishes (Chaetodon spp.). Fish Physiol Biochem 34, 483–492 (2008). https://doi.org/10.1007/s10695-008-9225-2

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