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Different expressions of trypsin and chymotrypsin in relation to growth in Atlantic salmon (Salmo salar L.)

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Abstract

The expressions of trypsin and chymotrypsin in the pyloric caeca of Atlantic salmon (Salmo salar L.) were studied in three experiments. Two internal (trypsin phenotypes, life stages) and three common external factors (starvation, feeding, temperatures) influencing growth rates were varied. Growth was stimulated by increased temperature and higher feeding rate, and it was depressed during starvation. The interaction between trypsin phenotype and start-feeding temperature affected specific activity of trypsin, but not of chymotrypsin. Trypsin specific activity and the activity ratio of trypsin to chymotrypsin (T/C ratio) increased when growth was promoted. Chymotrypsin specific activity, on the other hand, increased when there was a reduction in growth rate whereas fish with higher growth had higher chymotrypsin specific activity resulting in lower T/C ratio value. During a rapid growth phase, trypsin specific activity did not correlate with chymotrypsin specific activity. On the other hand, a relationship between specific activities of trypsin and chymotrypsin could be observed when growth declined, such as during food deprivation. Trypsin is the sensitive key protease under conditions favouring growth and genetically and environmentally affected, while chymotrypsin plays a major role when growth is limited or depressed. Trypsin specific activity and the T/C ratio value are shown to be important factors in the digestion process affecting growth rate, and could be applicable as indicators for growth studies of fish in captive cultures and in the wild, especially when food consumption rate cannot be measured.

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Acknowledgement

The authors would like to thank E. Bakke and M. Berntsen (previously technical assistants) for their contributions to the projects. These works were supported by the Institute of Marine Research in Norway, and by grants from the Ministry of Fisheries and the Research Council of Norway.

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Authors and Affiliations

  1. Institute of Marine Research –Matre, N-5984, Matredal, Norway

    K. Rungruangsak-Torrissen, R. Moss, L. H. Andresen & A. Berg

  2. National Institute of Nutrition and Seafood Research, P.O. Box 176, N-5804, Bergen, Norway

    R. Waagbø

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  1. K. Rungruangsak-Torrissen
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Rungruangsak-Torrissen, K., Moss, R., Andresen, L.H. et al. Different expressions of trypsin and chymotrypsin in relation to growth in Atlantic salmon (Salmo salar L.). Fish Physiol Biochem 32, 7–23 (2006). https://doi.org/10.1007/s10695-005-0630-5

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