Abstract
The fungus Pyrenophora teres causing disease symptoms similar to P. tritici-repentis was recently detected on wheat in Argentina. After confirmation by molecular studies the pathogen was identified as P. teres f. maculata, part of a complex of leaf spots that affect wheat and other cereal crops. The objective of this work was to characterize the virulence and identify QTL conferring resistance to two isolates of P. teres f. maculata (Ptm) in a collection of 110 spring wheat genotypes previously assembled for association mapping and genotyped with 2836 DArT markers. Two isolates of Ptm (PT2047 and PT2050) were used in field experiments. To find marker-trait associations (MTAs) a mixed linear model implemented in TASSEL 5.1 software was used. Considerable phenotypic variation in disease severity was observed at the seedling and adult stages, and some accessions were resistant to both isolates over 2 years. Twelve MTAs identified with nine markers were significantly associated with resistance. The nine markers were distributed over seven chromosomal regions on 6 of the 21 wheat chromosomes. These QTL were novel since this is the first study to identify genomic regions associated with resistance to Ptm in wheat. Wheat genotypes with moderate to high levels of resistance to P. teres f. maculata were identified and will be useful in breeding programs.
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References
Alvarez R, Steinbach HS (2009) A review of the effects of tillage systems on some soil physical properties, water content, nitrate availability and crops yield in the Argentine Pampas. Soil Tillage Res 104:1–5
Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Ser B (Methodol) 57:289–300
Danelli ALD, Reis EM, Fiallos FR (2011) Etiologia e intensidade de manchas foliares em cultivares de trigo em três locais do Rio Grande do Sul, Brasil. Sci Agropecu 2:149–155
Gurung S, Mamidi S, Bonman JM, Jackson EW, del Río LE, Acevedo M, Adhikari TB (2011) Association analysis of quantitative trait loci conferring tan spot resistance in spring wheat landraces. Theor Appl Genet 123:1029–1041
Liu Z, Ellwood SR, Oliver RP, Friesen TL (2011) Pyrenophora teres: profile of an increasingly damaging barley pathogen. Mol Plant Pathol 12:1–19
Maccaferri M, Sanguineti MC, Noli E, Tuberosa R (2005) Population structure and long-range linkage disequilibrium in a durum wheat elite collection. Mol Breed 15:271–290
Mikhailova LA, Ternyuk IG, Mironenko NV (2010) Pyrenophora teres, an agent causing wheat leaf spot. Microbiology 79:561–565
Moya P (2017) Antagonismo y efecto biocontrolador de Trichoderma spp. sobre Drechslera teres, agente causal de la "mancha en red" de la cebada (Hordeum vulgare L. var. vulgare). Doctoral dissertation, Facultad de Ciencias Naturales y Museo, National University of La Plata, Argentina
Muqaddasi QH, Lohwasser U, Nagel M, Börner A, Pillen K, Röder MS (2016) Genome-wide association mapping of anther extrusion in hexaploid spring wheat. PLoS ONE 11:e0155494
Patel JS, Mamidi S, Bonman JM, Adhikari TB (2013) Identification of QTL in spring wheat associated with resistance to a novel isolate of Pyrenophora tritici-repentis. Crop Sci 53:453–483
Perelló AE, Couretot L, Curti A, Uranga JP, Consolo VF (2019) First report of spot lesion of wheat caused by Pyrenophora teres f. sp maculata observed in Argentina. Crop Prot 122:19–22
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Quraishi UM, Pont C, Ain QU, Flores R, Burlot L, Alaux M, Quesneville H, Salse J (2017) Combined genomic and genetic data integration of major agronomical traits in bread wheat (Triticum aestivum L.). Front Plant Sci 8:1843
Raymond PJ, Bockus WW (1982) An in vitro technique for profuse sporulation of Drechslera tritici-repentis, (Abstr.). Phytopathology 72:934
Sheridan JE (1975) Helminthosporium diseases of cereals and their control. In: Close RC (Ed) Cereal seed diseases and their control. Proceedings of a seminar held at Lincoln College, New Zealand, July 8, pp 37–51
Tonin RB, Reis EM, Gómez DE (2015) Sensibilidad a fungicidas in vitro de micelios de Drechslera siccans aislada a partir de trigo. RIA Rev Investig Agropecu 41:192–200
Tóth B, Csősz M, Kopahnke D, Varga J (2008) First report on Pyrenophora teres causing lesions of wheat leaves in Hungary. Plant Pathol 57:385–385
Yu J, Pressoir G, Briggs WH, Vroh Bi I, Yamasaki M, Doebley JF, McMullen MD, Gaut BS, Nielsen DM, Holland JB et al (2006) A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nat Genet 38:203–208
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421
Zwart RS, Thompson JP, Milgate AW, Bansal UK, Williamson PM, Raman H, Bariana HS (2010) QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat. Mol Breed 26:107–124
Acknowledgements
We thank the staff at the J. Hirschhorn Experimental Station for technical assistance with the field experiments.
Funding
This study was funded by projects from ANPCYT, PICT2181/2010, CONICET PIP 217819/2014 and UNLP 227/2016.
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Uranga, J.P., Schierenbeck, M., Perelló, A.E. et al. Localization of QTL for resistance to Pyrenophora teres f. maculata, a new wheat pathogen. Euphytica 216, 56 (2020). https://doi.org/10.1007/s10681-020-02593-y
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DOI: https://doi.org/10.1007/s10681-020-02593-y