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QTL mapping for resistance to Cucurbit chlorotic yellows virus in melon (Cucumis melo L.)

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Abstract

Cucurbit chlorotic yellows virus (CCYV), a member of the genus Crinivirus, is transmitted by the whitefly Bemisia tabaci and causes yellowing of leaves of cucurbit plants. The melon accession JP 138332 is resistant to CCYV. Here, we detected a single QTL conferring resistance to CCYV in melon. Two susceptible accessions (‘Harukei No. 3’ and ‘AnMP-5’) were crossed with JP 138332 to generate two mapping populations. Each F2 population was evaluated for CCYV resistance in a greenhouse with the use of CCYV-infected B. tabaci, and genetic linkage maps were constructed using SSR and Indel markers. The map of the F2 population derived from ‘Harukei No. 3’, with 12 linkage groups, comprises 171 markers, which span 1538 cM, with an average interval between markers of 9.7 cM. The map of the F2 population derived from ‘AnMP-5’, also with 12 linkage groups, comprises 179 markers, which span 1419 cM, with an average interval of 8.5 cM. QTL analysis using each population detected one locus in the same region on chromosome 1, explaining 46.5–50% of the phenotypic variance. This is the first report of QTL mapping for CCYV resistance.

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References

  • Abrahamian PE, Sobh H, Abou-Jawdah Y (2012) First report of Cucurbit chlorotic yellows virus on cucumber in Lebanon. Plant Dis 96:1704

    Article  Google Scholar 

  • Al-Saleh MA, Al-Shahwan IM, Amer MA, Shakeel MT, Abdalla OA, Orfanidou CG, Katis NI (2015) First report of Cucurbit chlorotic yellows virus in cucumber in Saudi Arabia. Plant Dis 99:734

    Article  Google Scholar 

  • Amer MA (2015) Serological and molecular characterization of Cucurbit chlorotic yellows virus affecting cucumber plants in Egypt. Int J Virol 11:1–11

    Article  CAS  Google Scholar 

  • Bananej K, Menzel W, Kianfar N, Vahdat A, Winter S (2013) First report of Cucurbit chlorotic yellows virus infecting cucumber, melon, and squash in Iran. Plant Dis 97:1005

    Article  Google Scholar 

  • Brotman Y, Normantovich M, Goldenberg Z, Zvirin Z, Kovalski I, Stovbun N, Doniger T, Bolger AM, Troadec C, Bendahmane A, Cohen R, Katzir N, Pitrat M, Dogimont C, Perl-Treves R (2013) Dual resistance of melon to Fusarium oxysporum races 0 and 2 and to Papaya ring-spot virus is controlled by a pair of head-to-head-oriented NB-LRR genes of unusual architecture. Mol Plant 6:235–238

    Article  CAS  Google Scholar 

  • Danin-Poleg Y, Reis N, Tzuri G, Katzir N (2001) Development and characterization of microsatellite markers in Cucumis. Theor Appl Genet 102:61–72

    Article  CAS  Google Scholar 

  • Dogimont C, Chovelon V, Pauquet J, Boualem A, Bendahmane A (2014) The Vat locus encodes for a CC-NBS-LRR protein that confers resistance to Aphis gossypii infestation and A. gossypii-mediated virus resistance. Plant J 80:993–1004

    Article  CAS  Google Scholar 

  • Esteva J, Nuez F (1992) Tolerance to a whitefly-transmitted virus causing muskmelon yellows disease in Spain. Theor Appl Genet 84:693–697

    Article  CAS  Google Scholar 

  • Fazio G, Staub JE, Chung SM (2002) Development and characterization of PCR markers in cucumber. J Am Soc Hort Sci 127:545–557

    CAS  Google Scholar 

  • Fernandez-Silva I, Eduardo I, Blanca J, Esteras C, Picó B, Nuez F, Arús P, Garcia-Mas J, Monforte AJ (2008) Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.). Theor Appl Genet 118:139–150. https://doi.org/10.1007/s00122-008-0883-3

    Article  CAS  PubMed  Google Scholar 

  • Fukino N, Sakata Y, Kunihisa M, Matsumoto S (2007) Characterization of novel simple sequence repeat (SSR) markers for melon (Cucumis melo L.) and their use for genotype identification. J Hort Sci Biotech 82:330–334

    Article  CAS  Google Scholar 

  • Fukino N, Ohara T, Monforte AJ, Sugiyama M, Sakata Y, Kunihisa M, Matsumoto S (2008) Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.). Theor Appl Genet 118:165–175

    Article  CAS  Google Scholar 

  • Garcia-Mas J, Benjak A, Sanseverino W, Bourgeois M et al (2012) The genome of melon (Cucumis melo L.). Proc Natl Acad Sci USA 109:11872–11877

    Article  CAS  Google Scholar 

  • Giner A, Pascual L, Bourgeois M, Gyetvai G, Rios P, Picó B, Troadec C, Bendahmane A, Garcia-Mas J, Martín-Hernández AM (2017) A mutation in the melon Vacuolar Protein Sorting 41 prevents systemic infection of Cucumber mosaic virus. Sci Rep 7:10471

    Article  Google Scholar 

  • Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arús P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811

    Article  CAS  Google Scholar 

  • Gu QS, Liu YH, Wang YH, Huangfu WG, Gu HF, Xu L, Song FM, Brown JK (2011) First report of Cucurbit chlorotic yellows virus in cucumber, melon, and watermelon in China. Plant Dis 95:73

    Article  Google Scholar 

  • Gyoutoku Y, Okazaki S, Furuta A, Etoh T, Mizobe M, Kuno K, Hayashida S, Okuda M (2009) Chlorotic yellows disease of melon caused by Cucurbit chlorotic yellows virus, a new crinivirus. Jpn J Phytopathol 75:109–111 (In Japanese with English abstract)

    Article  Google Scholar 

  • Hamed K, Menzel W, Dafalla G, Gadelseed AMA, Winter S (2011) First report of Cucurbit chlorotic yellows virus infecting muskmelon and cucumber in Sudan. Plant Dis 95:1321

    Article  Google Scholar 

  • Hashimoto M, Neriya Y, Yamaji Y, Namba S (2016) Recessive resistance to plant viruses: potential resistance genes beyond translation initiation factors. Front Microbiol 7:1695

    Article  Google Scholar 

  • Huang LH, Tseng HH, Li JT, Chen TC (2010) First report of Cucurbit chlorotic yellows virus infecting cucurbits in Taiwan. Plant Dis 94:1168

    Article  Google Scholar 

  • Kosambi D (1943) The estimation of map distances from recombination values. Ann Eugen 12(1):172–175

    Article  Google Scholar 

  • Lander E, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S, Newberg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  Google Scholar 

  • López-Sesé AI, Gómez-Guillamón ML (2000) Resistance to Cucurbit yellowing stunting disorder virus (CYSDV) in Cucumis melo L. HortScience 35:110–113

    Google Scholar 

  • Marco CF, Aguilar JM, Abad J, Gómez-Guillamón ML, Aranda MA (2003) Melon resistance to Cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. Phytopathology 93:844–852

    Article  Google Scholar 

  • McCreight JD (2000) Inheritance of resistance to lettuce infectious yellows virus in melon. HortScience 35:1118–1120

    Google Scholar 

  • McCreight JD, Wintermantel WM (2008) Potential new sources of genetic resistance in melon to Cucurbit yellow stunting disorder virus. In: Pitrat M (ed) Cucurbitaceae 2008, IXth Eucarpia meeting on genetics and breeding of cucurbitaceae. INRA, Avignon, pp 173–179

    Google Scholar 

  • McCreight JD, Wintermantel WM (2011) Genetic resistance in melon PI 313970 to Cucurbit yellow stunting disorder virus. HortScience 46:1582–1587

    CAS  Google Scholar 

  • McCreight JD, Wintermantel WM, Natwick ET, Sinclair JW, Crosby KM, Gómez-Guillamón ML (2017) Recessive resistance to CYSDV in melon TGR 1551. Acta Hortic 1151:101–108

    Article  Google Scholar 

  • McHale L, Tan X, Koehl P, Michelmore RW (2006) Plant NBS-LRR proteins: adaptable guards. Genome Biol 7:212

    Article  Google Scholar 

  • Nieto C, Morales M, Orjeda G, Clepet C, Monfort A, Truniger V, Sturbois B, Arus P, Caboche M, Puigdomenech P, Pitrat M, Dogimont C, Garcia-Mas J, Aranda M, Bendahmane A (2006) An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon. Plant J 48:452–462

    Article  CAS  Google Scholar 

  • Okuda M, Okazaki S, Yamasaki S, Okuda S, Sugiyama M (2010) Host range and complete genome sequence of Cucurbit chlorotic yellows virus, a new member of the genus Crinivirus. Phytopathology 100:560–566

    Article  CAS  Google Scholar 

  • Okuda S, Okuda M, Sugiyama M, Sakata Y, Takeshita M, Iwai H (2013) Resistance in melon to Cucurbit chlorotic yellows virus, a whitefly-transmitted crinivirus. Eur J Plant Pathol 135:313–321

    Article  CAS  Google Scholar 

  • Orfanidou C, Maliogka VI, Katis NI (2014) First report of Cucurbit chlorotic yellows virus in cucumber, melon, and watermelon in Greece. Plant Dis 98:1446

    Article  Google Scholar 

  • Orfanidou CG, Maliogka VI, Katis NI, Kontosfyris G, Smith T, Caglayan K (2017) First report of Cucurbit chlorotic yellows virus in cucumber in Turkey. J Plant Pathol 99:533

    Google Scholar 

  • Ritschel PS, Lins TCL, Tristan RL, Buso GSC, Buso JA, Ferreira ME (2004) Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biol 4:9

    Article  Google Scholar 

  • Ríus FJP, Garcés-Claver A, Gómez-Guillamón ML (2016) Detection of two QTLs associated with resistance to Cucurbit yellow stunting disorder virus in melon line TGR 1551. Cucurbitaceae 2016, XIth Eucarpia Meeting on Cucurbit Genetics and Breeding, Warsaw, Poland, pp 334–337

  • Sekhwal MK, Li P, Lam I, Wang X, Cloutier S, You FM (2015) Disease resistance gene analogs (RGAs) in plants. Int J Mol Sci 16:19248–19290

    Article  CAS  Google Scholar 

  • Shimizu T, Yano K (2011) A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag). BMC Res Notes 26:161

    Article  Google Scholar 

  • Wang S, Basten CJ, Zeng ZB (2012) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, USA. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm

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Acknowledgements

We thank Dr. Kenji Kubota (Central Region Agricultural Research Center, NARO) for confirming that the B. tabaci population carried CCYV before our inoculation tests.

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Author notes

  1. Shiori Okuda

    Present address: Institute of Agrobiological Sciences, NARO, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan

Authors and Affiliations

  1. Institute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NARO), 360 Kusawa, Ano, Tsu, Mie, 514-2392, Japan

    Yoichi Kawazu, Koichiro Shimomura & Mitsuhiro Sugiyama

  2. Kyushu Okinawa Agricultural Research Center, NARO, 1823-1 Miimachi, Kurume, Fukuoka, 839-8503, Japan

    Shoichi Maeda & Yoichi Yamato

  3. Hokkaido Agricultural Research Center, NARO, 9-4 Shinseiminami, Memuro, Kasai, Hokkaido, 082-0081, Japan

    Shigenori Ueda

  4. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan

    Shiori Okuda

  5. Central Region Agricultural Research Center, NARO, 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Mitsuru Okuda

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  1. Yoichi Kawazu
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Correspondence to Mitsuhiro Sugiyama.

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Kawazu, Y., Shimomura, K., Maeda, S. et al. QTL mapping for resistance to Cucurbit chlorotic yellows virus in melon (Cucumis melo L.). Euphytica 214, 239 (2018). https://doi.org/10.1007/s10681-018-2321-1

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  • DOI: https://doi.org/10.1007/s10681-018-2321-1

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