Abstract
Tobacco mosaic virus (TMV) caused serious loss in yield and quality of tobacco every year. It is a long-term goal to improve the tobacco resistance against TMV by tobacco breeding. N gene was the firstly reported TMV-resistant gene, which showed resistance against all Tobamoviruses except the Ob stain and belonged to the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance (R) genes. At present, N gene had already been widely used in tobacco conventional breeding, but there is rare available molecular maker used in marker-assisted selection of TMV resistance. In this study, we designed a pair of primers that specific amplify N gene fragment based on the sequence of N gene intron III, named N-marker. Then, we identified TMV resistance by two selecting methods, PCR with N-marker and inoculated with the TMV-C strain. Results from the two method showed that (1) 13 varieties among 67 tobacco varieties displayed hypersensitive reaction when inoculated with the TMV-C strain, also contained N gene fragments screened by PCR with N-marker; (2) 105 strains of 200 BC1 strains showed resistance against TMV when inoculated with TMV-C strain, meanwhile, 103 of the 105 strains contained N gene fragment verified by PCR with N-marker. Therefore, the N-marker is reliable for high throughput screening of germplasm resources and tobacco breeding materials in selection of N-mediated TMV resistance. Our study not only developed a molecular marker for tobacco breeding, but also identified new germplasm resources that are resistant to TMV.
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Acknowledgements
This work was supported by a Grant from the Tobacco Genome Project of China National Tobacco Company (110201301008), the Science Foundation for Young Scientists of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences (2016B04) and Shandong Provincial Natural Science Foundation, China (ZR2016CB39).
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Yu, Z., Liuying, W., Aiguo, Y. et al. Development and application of a molecular marker for TMV resistance based on N gene in tobacco (Nicotiana tabacum). Euphytica 213, 259 (2017). https://doi.org/10.1007/s10681-017-2044-8
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DOI: https://doi.org/10.1007/s10681-017-2044-8