Skip to main content

Advertisement

SpringerLink
Log in

Soybean cadmium concentration: validation of a QTL affecting seed cadmium accumulation for improved food safety

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Soybean can accumulate considerable amounts of cadmium depending on soil conditions. As low cadmium concentration of soybean is of great interest in soy food production, a major quantitative trait locus described earlier for controlling cadmium accumulation in the seed was utilized to classify early maturity genotypes with respect to cadmium uptake and to verify their cadmium accumulation level. Marker-selected soybean genotypes were then tested for cadmium accumulation in a pot experiment with three cadmium levels and at three field locations in Austria. Out of 48 soybean genotypes, 19 exhibited the microsatellite marker allele associated with low cadmium accumulation. In the pot experiment, seed cadmium concentration was below 0.15 mg kg−1 in low cadmium accumulating genotypes while it reached over 0.30 mg kg−1 in genotypes carrying the high cadmium allele. In field experiments, cadmium concentration was in the range between 0.03 and 0.16 mg kg−1, and both the cadmium accumulation locus as well as the experimental locations had a significant influence on seed cadmium. Marker-assisted selection of soybean genotypes for low cadmium accumulation could thus contribute to improved food safety which is particularly important for environments with unknown cadmium status of soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adams SV, Newcomb PA, Shafer MM, Atkinson C, Aiello Bowles EJ, Newton KM, Lampe JW (2011) Sources of cadmium exposure among healthy premenopausal women. Sci Total Environ 409:1632–1637

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Al Sayegh Petkovšek S, Pokorny B (2013) Lead and cadmium in mushrooms from the vicinity of two large emission sources in Slovenia. Sci Total Environ 443:944–954

    Article  Google Scholar 

  • Arao T, Ae N, Sugiyama M, Takahashi M (2003) Genotypic differences in cadmium uptake and distribution in soybeans. Plant Soil 251:247–253

    Article  CAS  Google Scholar 

  • Benitez ER, Hajika M, Yamada T, Takahashi K, Oki N, Yamada N, Nakamura T, Kanamura K (2010) A major QTL controlling seed cadmium accumulation in soybean. Crop Sci 50:1728–1734

    Article  CAS  Google Scholar 

  • Benitez ER, Hajika M, Takahashi R (2012) Single-base substitution in P1B-ATPase gene is associated with a major QTL for seed cadmium concentration in soybean. J Hered 103:278–286

    Article  CAS  PubMed  Google Scholar 

  • Chen KI, Erh MH, Su NW, Liu WH, Chou CC, Cheng KC (2012) Soyfoods and soybean products: from traditional use to modern applications. Appl Microbiol Biotechnol 96:9–22

    Article  CAS  PubMed  Google Scholar 

  • European Commission (2006) Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union, L364/5 of 20.12.2006

  • Greger M, Löfstedt M (2004) Comparison of uptake and distribution of cadmium in different cultivars of bread and durum wheat. Crop Sci 44:501–507

    Article  CAS  Google Scholar 

  • Järup L, Åkesson A (2009) Current status of cadmium as an environmental health problem. Toxicol Appl Pharm 238:201–208

    Article  Google Scholar 

  • Jegadeesan S, Yu K, Poysa V, Gawalko E, Morrison MJ, Shi C, Cober E (2010) Mapping and validation of simple sequence repeat markers linked to a major gene controlling seed cadmium accumulation in soybean [Glycine max (L.) Merr]. Theor Appl Genet 121:283–294

    Article  CAS  PubMed  Google Scholar 

  • Li X, Zhang L, Li Y, Ma L, Bu N, Ma C (2012) Changes in photosynthesis, antioxidant enzymes and lipid peroxidation in soybean seedlings exposed to UV-B radiation and/or Cd. Plant Soil 352:377–387

    Article  CAS  Google Scholar 

  • Mansur LM, Lark KG, Kross H, Oliveira A (1993) Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Theor Appl Genet 86:907–913

    CAS  PubMed  Google Scholar 

  • McLaughlin MJ, Maier NA, Rayment GE, Sparrow LA, Berg G, McKay A, Milham P, Merry RH, Smart MK (1997) Cadmium in Australian potato tubers and soils. J Environ Qual 26:1644–1649

    Article  CAS  Google Scholar 

  • McLaughlin MJ, Parker DR, Clarke JM (1999) Metals and micronutrients—food safety issues. Field Crops Res 60:143–163

    Article  Google Scholar 

  • Meharg AA, Norton G, Deacon C, Williams P, Adomako EE, Price A, Zhu Y, Li G, Zhao FJ, McGrath S, Villada A, Sommella A, Mangala P, De Silva CS, Brammer H, Dasgupta T, Islam MR (2013) Variation in rice cadmium related to human exposure. Environ Sci Technol 47:5613–5618

    Article  CAS  PubMed  Google Scholar 

  • Moon KH, Kim JY, Chang MI, Kim US, Kim SJ, Baek SH (2013) Heavy metal and amino acid contents of soybean by application of sewage and industrial sludge. J Korean Soc Food Sci Nutr 42:268–277

    Article  CAS  Google Scholar 

  • Öborn I, Jansson G, Johnsson L (1995) A field study on the influence of soil pH on trace element levels in spring wheat (Triticum aestivum), potatoes (Solanum tuberosum) and carrots (Daucus carota). Water Air Soil Pollut 85:835–840

    Article  Google Scholar 

  • Salazar MJ, Rodriguez JH, Nieto GL, Pignata ML (2012) Effects of heavy metal concentrations (Cd, Zn and Pb) in agricultural soils near different emission sources on quality, accumulation and food safety in soybean [Glycine max (L.) Merrill]. J Hazard Mater 233–234:244–253

    Article  PubMed  Google Scholar 

  • SAS (1988) SAS STAT user’s guide, release 6.03. SAS Institute, Cary

    Google Scholar 

  • Sato T, Zahlner V, Berghofer E, Lošák T, Vollmann J (2012) Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples. Plant Breed 131:531–534

    Article  CAS  Google Scholar 

  • Shute T, Macfie SM (2006) Cadmium and zinc accumulation in soybean: a threat to food safety. Sci Total Environ 371:63–73

    Article  CAS  PubMed  Google Scholar 

  • Spiegel H, Sager M, Oberforster M, Mechtler K, Stüger HP, Baumgarten A (2009) Nutritionally relevant elements in staple foods: influence of arable site versus choice of variety. Environ Geochem Health 31:549–560

    Article  CAS  PubMed  Google Scholar 

  • Sugiyama M, Ae N, Arao T (2007) Role of roots in differences in seed cadmium concentration among soybean cultivars—proof by grafting experiment. Plant Soil 295:1–11

    Article  CAS  Google Scholar 

  • Vollmann J, Walter H, Sato T, Schweiger P (2011) Digital image analysis and chlorophyll metering for phenotyping the effects of nodulation in soybean. Comput Electron Agric 75:190–195

    Article  Google Scholar 

  • Wang X, Jiang GL, Green M, Scott RA, Hyten DL, Cregan PB (2012a) Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean. Mol Breed 30:1163–1179

    Article  Google Scholar 

  • Wang Y, Yu KF, Poysa V, Shi C, Zhou YH (2012b) A single point mutation in GmHMA3 affects cadmium (Cd) translocation and accumulation in soybean seeds. Molec Plant 5:1154–1156

    Article  CAS  Google Scholar 

  • Xiao CW (2008) Health effects of soy protein and isoflavones in humans. J Nutr 138:1244 S–1249 S

    CAS  Google Scholar 

  • Zhou Y, Xue M, Yang Z, Gong Y, Yuan J, Zhou C, Huang B (2013) High cadmium pollution risk on vegetable amaranth and a selection for pollution-safe cultivars to lower the risk. Front Environ Sci Eng 7:219–230

    Article  CAS  Google Scholar 

  • Zhuang P, Li ZA, Zou B, Xia HP, Wang G (2013) Heavy metal contamination in soil and soybean near the Dabaoshan mine, South China. Pedosphere 23:298–304

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was partly supported by project no. 63p4 of the joint AKTION Austria—Czech Republic programme.

Author information

Authors and Affiliations

  1. Division of Plant Breeding, Department of Crop Sciences, University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria

    Johann Vollmann, Martin Pachner & Daisuke Watanabe

  2. Department of Environmentalistics and Natural Resources, Faculty of Regional Development and International Studies, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Tomáš Lošák, Ludmila Musilová & Jaroslav Hlušek

Authors
  1. Johann Vollmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tomáš Lošák
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martin Pachner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daisuke Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ludmila Musilová
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jaroslav Hlušek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Johann Vollmann.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vollmann, J., Lošák, T., Pachner, M. et al. Soybean cadmium concentration: validation of a QTL affecting seed cadmium accumulation for improved food safety. Euphytica 203, 177–184 (2015). https://doi.org/10.1007/s10681-014-1297-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1297-8

Keywords

Search

Navigation