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Characterization of Phytophthora resistance in soybean cultivars/lines bred in Henan province

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Abstract

Deployment of resistant soybean cultivars is the most effective and economical method of controlling Phytophthora root rot (PRR) incited by Phytophthora sojae, and characterization of Phytophthora resistance of the soybean cultivars greatly facilitates the effective utilization. The objective of this study was to characterize the resistance phenotype in 30 soybean cultivars/lines bred in Henan province and 4 ancestral cultivars which were inoculated with 26 P. sojae pathotypes. The 34 soybean cultivars/lines showed 34 different reaction types of resistance to 26 P. sojae pathotypes. The reaction types produced on the cultivars/lines were compared with those produced on the differential lines to postulate which Rps gene was present. The gene Rps5 and Rps3a or gene combination Rps3a+5 were postulated to be present in Zhoudou17 and Zheng77249, respectively. The other 32 cultivars/lines exhibited novel reaction types that were different from known single or two Rps gene combinations. The cluster analysis of the reaction types revealed 10 groups among the 34 soybean cultivars/lines, 17 differentials and the cultivar Williams at the similarity coefficient 0.6540. This study indicated that Phytophthora resistance was extremely diverse in this region. The cultivars/lines with broad spectrum resistance could provide effective sources of resistance for the control of PRR in the future.

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Abbreviations

D:

Day

DPI:

Days post inoculation

I:

Intermediate

PRR:

Phytophthora root rot

R:

Resistant

Rps :

Resistance to Phytophthora sojae

S:

Susceptible

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Acknowledgments

We thank Professor Li Weidong and Dr. Weiguo Lu (Henan Academy of Agricultural Sciences, China) for providing the soybean cultivars/lines used in this study. The work was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303018) and the Program of Protection of Crop Germplasm Resources (NB2010-2130135-25-14) from the Ministry of Agriculture of the People’s Republic of China.

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Authors and Affiliations

  1. MOA Key Lab of Soybean Biology (Beijing), The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, People’s Republic of China

    Jiqing Zhang, Suli Sun, Canxing Duan, Xiaoming Wang, Xiaofei Wu & Zhendong Zhu

  2. Agricultural Science, Liaocheng University, Liaocheng, 252059, People’s Republic of China

    Guiqing Wang

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  1. Jiqing Zhang
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  2. Suli Sun
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  3. Guiqing Wang
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  4. Canxing Duan
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  5. Xiaoming Wang
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  6. Xiaofei Wu
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  7. Zhendong Zhu
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Correspondence to Zhendong Zhu.

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Zhang, J., Sun, S., Wang, G. et al. Characterization of Phytophthora resistance in soybean cultivars/lines bred in Henan province. Euphytica 196, 375–384 (2014). https://doi.org/10.1007/s10681-013-1040-x

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  • DOI: https://doi.org/10.1007/s10681-013-1040-x

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