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Meiotic behavior as a selection tool in the breeding of Brachiaria humidicola (Poaceae)

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Abstract

Brachiaria humidicola is a tropical grass that grows in seasonally swampy grasslands in Africa. In Brazil, two apomictic cultivars (2n = 54) of this species are widely used as pastures in poorly drained soils. The recent discovery of a sexual polyploid accession (2n = 36) in the germplasm collection at the Embrapa Beef Cattle Research Center allowed intraspecific hybridization with the objective of broadening the genetic variability and selection of superior genotypes in this species. Hybridization, however, depends on accessions with the same ploidy level. Cytological analyses of 55 accessions revealed that 19 apomictic accessions also presented 2n = 36 chromosomes. Chromosome pairing in hexavalent association at diakinesis and metaphase I suggested that the basic chromosome number for this species is x = 6. Cytological analysis revealed abnormalities in variable frequencies in the meiosis of these hexaploid (2n = 6x = 36) accessions. The most common were those related to irregular chromosome segregation which led to unbalanced gamete formation, but chromosome stickiness was also recorded. These results clearly demonstrate the value of cytogenetics in the choice of genitors and for superior hybrids to be obtained in the breeding of this species. For that both the ploidy level and the frequency of abnormalities need to be considered, besides other favorable agronomic characteristics.

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References

  • Adamowski EV, Boldrini KR, Pagliarini MS, Valle CB (2008a) Abnormal cytokinesis in microsporogenesis of Brachiaria humidicola (Poaceae: Paniceae). Genet Mol Res 6:616–621

    Google Scholar 

  • Adamowski EV, Pagliarini MS, Valle CB (2008b) Meiotic behavior in three interspecific three way hybrids between Brachiaria ruziziensis and B. brizantha (Poaceae: Paniceae). J Genet 87:33–38

    Article  PubMed  Google Scholar 

  • Argel PJ, Keller-Grein G (1996) Regional experience with Brachiaria: tropical America-humid lowlands. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT/Embrapa, Colombia, pp 205–224

    Google Scholar 

  • Barbosa JD, Oliveira CMC, Tokarnia CH, Peixoto PV (2006) Hepatogenous photosensitization in horses caused by Brachiaria humidicola (Gramineae) in the state of Pará. Pesq Vet Bras 26:147–153

    Article  Google Scholar 

  • Basappa GP, Muniyamma MS, Chinnappa CC (1987) An investigation of chromosome numbers in the genus Brachiaria (Poaceae: Paniceae) in relation to morphology and taxonomy. Can J Bot 65:2297–2309

    Article  Google Scholar 

  • Bernini C, Marin-Morales MA (2001) Karyotype analysis in Brachiaria (Poaceae) species. Cytobios 104:157–171

    PubMed  CAS  Google Scholar 

  • Boldrini KR, Pagliarini MS, Valle CB (2006) Abnormal timing of cytokinesis in microsporogenesis of Brachiaria humidicola (Poaceae: Paniceae). J Genet 85:225–228

    Article  PubMed  Google Scholar 

  • Boldrini KR, Micheletti PL, Gallo PH, Mendes-Bonato AB, Pagliarini MS, Valle CB (2009a) Origin of a polyploidy accession of Brachiaria humidicola (Poaceae: Panicoideae: Paniceae). Genet Mol Res 8:888–895

    Article  PubMed  CAS  Google Scholar 

  • Boldrini KR, Pagliarini MS, Valle CB (2009b) Meiotic behavior of a nonaploid accession endorses x = 6 for Brachiaria humidicola (Poaceae). Genet Mol Res 8:1444–1450

    Article  PubMed  CAS  Google Scholar 

  • Boldrini KR, Pagliarini MS, Valle CB (2010) Evidence of natural hybridization in Brachiaria humidicola (Rendle) Shweick. (Poaceae: Panicoideae: Paniceae). J Genet 89:91–94

    Article  PubMed  Google Scholar 

  • Calisto V, Fuzinatto VA, Message HJ, Mendes-Bonato AB, Boldrini KR, Pagliarini MS, Valle CB (2008) Desynapsis and precocious cytokinesis in Brachiaria humidicola (Poaceae) compromise meiotic division. J Genet 87:27–34

    Article  PubMed  Google Scholar 

  • Dias Filho MB (2002) Tolerance to flooding in five Brachiaria brizantha accessions. Pesq Agrop Bras 36:439–447

    Article  Google Scholar 

  • Felismino MF, Pagliarini MS, Valle CB (2010) Meiotic behavior of interspecific hybrids between artificially tetraploidized sexual Brachiaria ruziziensis and tetraploid apomictic B. brizantha (Poaceae). Sci Agric 67:191–197

    Article  Google Scholar 

  • Ferguson JE (1974) Method of reproduction in Brachiaria ruziziensis Germain et Evrard. Cornell Univ. PhD Thesis, 149 p

  • Fuzinatto VA, Pagliarini MS, Valle CB (2007) Microsporogenesis in sexual Brachiaria hybrids (Poaceae). Genet Mol Res 6:1107–1117

    PubMed  CAS  Google Scholar 

  • Gallo PH, Micheletti PL, Boldrini KR, Risso-Pascotto C, Pagliarini MS, Valle CB (2007) 2n gamete formation in the genus Brachiaria (Poaceae: Paniceae). Euphytica 154:255–260

    Article  Google Scholar 

  • Keller-Grein G, Maass BL, Hanson J (1996) Natural variation in Brachiaria and existing germoplasm collections. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT/Embrapa, Colombia, pp 17–42

    Google Scholar 

  • Lutts S, Ndikumana J, Louant BP (1991) Fertlity of Brachiaria ruziziensis in interspecific crosses with Brachiaria decumbens and Brachiaria brizantha: meiotic behavior, pollen viability and seed set. Euphytica 57:267–274

    Article  Google Scholar 

  • Mendes-Bonato AB, Pagliarini MS, Valle CB, Penteado MIO (2001) A severe case of chromosome stickiness in pollen mother cells of Brachiaria brizantha (Hochst.) Stapf (Gramineae). Cytologia 66:287–291

    Article  Google Scholar 

  • Mendes-Bonato AB, Pagliarini MS, Forli F, Valle CB, Penteado MIO (2002) Chromosome number and microsporogenesis in Brachiaria brizantha (Gramineae). Euphytica 125:419–425

    Article  Google Scholar 

  • Mendes-Bonato AB, Pagliarini MS, Risso-Pascotto C, Valle CB (2006) Chromosome number and meiotic behavior in Brachiaria jubata (Gramineae). J Genet 85:83–88

    Article  PubMed  Google Scholar 

  • Miles JW, Maass BL, Valle CB (1996) Brachiaria: biology, agronomy, and improvement. CIAT/Embrapa, Colombia, p 288

    Google Scholar 

  • Miles JW, Valle CB, Rao IM, Euclides VPB (2004) Brachiariagrasses. In: Sollenberger L (ed) Warm-season (C4) grasses. ASA, CSSA, SSSA, Madison, pp 745–760

    Google Scholar 

  • Naumova TN, Hayward MD, Wagenvoort M (1999) Apomixis and sexuality in diploid and tetraploid accessions of Brachiaria decumbens. Sex Plant Reprod 12:43–52

    Article  Google Scholar 

  • Pagliarini MS, Risso-Pascotto C, Souza-Kaneshima AM, Valle CB (2008) Analysis of meiotic behavior in selecting potential genitors among diploid and artificially induced tetraploid accessions of Brachiaria ruziziensis (Poaceae). Euphytica 164:181–187

    Article  Google Scholar 

  • Pritchard AJ (1967) Apomixis in Brachiaria decumbens Stapf. J Austr Inst Agric Sci 33:264–265

    Google Scholar 

  • Renvoize SA, Clayton WD, Kabuye CHS (1996) Morphology, taxonomy, and natural distribution of Brachiaria (Trin.) Griseb. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT/Embrapa, Colombia, pp 1–15

    Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB, Mendes-Bonato AB (2003) Chromosome number and microsporogenesis in a pentaploid accession of Brachiaria brizantha (Gramineae). Plant Breed 122:136–140

    Article  Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB (2005) Meiotic behavior in interspecific hybrids between Brachiaria ruziziensis and Brachiaria brizantha (Poaceae). Euphytica 145:155–159

    Article  Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB (2006a) Microsporogenesis in Brachiaria dictyoneura (Fig. & De Not.) Stapf (Poaceae: Paniceae). Genet Mol Res 5:837–845

    PubMed  CAS  Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB (2006b) A new basic chromosome number for the genus Brachiaria (Trin.) Griseb. (Poaceae: Panicoideae: Paniceae). Genet Res Crop Evol 53:7–10

    Article  Google Scholar 

  • Schank SC, Sotomayor-Ríos A (1968) Cytological studies on Brachiaria species. Soil Crop Sci Soc Fla Proc 28:156–162

    Google Scholar 

  • Sotomayor-Rios A, Velez-Fortuno J, Woodbury R, Schertz KF, Sierra-Bracero A (1960) Description and cytology of a form of signal grass (Brachiaria brizantha Stapf) and its agronomic behaviour compared to Guinea grass (Panicum maximum Jacq.). J Agr Univ Puerto-Rico 44:208–220

    CAS  Google Scholar 

  • Subbarao GV, Rondon M, Ito O, Ishikawa T, Rao IM, Nakahara K, Lascano C, Berry WL (2007) Biological nitrification inhibition (BNI)—is it a wdespread phenomenon? Plant Soil 294:5–18

    Article  CAS  Google Scholar 

  • Utsunomiya KS, Pagliarini MS, Valle CB (2005) Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho & Hiern) Stapf (Gramineae). Biocell 29:295–301

    PubMed  CAS  Google Scholar 

  • Valle CB, Glienke C (1991) New sexual accession in Brachiaria. Apomixis Newsletter 7:42–43

    Google Scholar 

  • Valle CB, Pagliarini MS (2009) Biology, cytogenetics and breeding of Brachiaria. In: Singh RJ (ed) Genetic resources, chromosome engineering, and crop improvement. CRC Press, Boca Raton, pp 103–151

    Chapter  Google Scholar 

  • Valle CB, Savidan YH (1996) Genetics, cytogenetics, and reproductive biology of Brachiaria. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT/Embrapa, Colombia, pp 147–163

    Google Scholar 

  • Young BA, Sherwood RT, Bashaw EC (1979) Cleared-pistyl and thick-sectioning techniques for detecting aposporous apomixis in grasses. Can J Bot 57:1668–1672

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to UNIPASTO and CNPq for financial support.

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, State University of Maringá, Maringá, PR, 87020-900, Brazil

    Kellen Regina Boldrini, Eleniza de Victor Adamowski, Hugo Message, Vergílio Calisto & Maria Suely Pagliarini

  2. Embrapa Beef Cattle Research Center, Campo Grande, MS, Brazil

    Cacilda Borges do Valle

Authors
  1. Kellen Regina Boldrini
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  2. Eleniza de Victor Adamowski
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  3. Hugo Message
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  4. Vergílio Calisto
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  5. Maria Suely Pagliarini
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  6. Cacilda Borges do Valle
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Corresponding author

Correspondence to Maria Suely Pagliarini.

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Boldrini, K.R., de Victor Adamowski, E., Message, H. et al. Meiotic behavior as a selection tool in the breeding of Brachiaria humidicola (Poaceae). Euphytica 182, 317–324 (2011). https://doi.org/10.1007/s10681-011-0402-5

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  • DOI: https://doi.org/10.1007/s10681-011-0402-5

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