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Molecular mapping of a gene conferring resistance to Aphanomyces root rot (black root) in sugar beet (Beta vulgaris L.)

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Abstract

Caused by Aphanomyces cochlioides Drechsler, Aphanomyces root rot is a serious disease of sugar beet (Beta vulgaris L.), for which sources of resistance are scarce. To identify the segregation pattern of the rare resistance trait found in Japanese sugar beet line ‘NK-310mm-O’, F1 and BC1F2 seedings, drawn from a cross between ‘NK-310mm-O’ and susceptible line ‘NK-184mm-O’, were inoculated with zoospores and their survival evaluated in the greenhouse. Resistance segregation followed was that of a single dominant gene, which was designated Acr1 (Aphanomyces cochlioides resistance 1). Molecular markers tightly linked to Acr1 were identified by bulked segregant analysis of two BC1F2 populations. Fourteen AFLP markers linked to Acr1 were identified, the closest located within ±3.3 cM. Three F5 lines and two BC2F1 lines, selected on the basis of their Acr1-AFLP markers, were tested for their resistance to Aphanomyces root rot in a highly infested field. Results indicated that Acr1 conferred significant resistance to Aphanomyces root rot at the field level. Based on its linkage with CAPS marker tk, a representative marker for chromosome III, Acr1 was located on this chromosome. The clear linkage between tk and Rhizomania resistance trait Rz1, suggests the clustering of major disease resistance genes on chromosome III.

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Acknowledgements

The authors wish to thank Chiho Tanaka, Sachiko Nishimura and Naoko Yamada for technical assistance. This work was supported in part by the grant from the Ministry of Agriculture, Forestry and Fisheries, Japan, (Research and development projects applied in promoting new Agriculture Forestry and Fisheries policies, No. 18058), and the Program for Promotion of Basic and Applied Research for Innovation in Bio-oriented Industry (BRAIN).

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Authors and Affiliations

  1. Memuro Upland Farming Research Station, National Agricultural Research Center for Hokkaido region, (NARCH), Hokkaido, Japan

    Kazunori Taguchi, Kazuyuki Okazaki & Hiroyuki Takahashi

  2. Laboratory of Genetic Engineering, Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan

    Tomohiko Kubo & Tetsuo Mikami

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  1. Kazunori Taguchi
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  2. Kazuyuki Okazaki
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  3. Hiroyuki Takahashi
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  4. Tomohiko Kubo
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  5. Tetsuo Mikami
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Correspondence to Kazunori Taguchi.

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Taguchi, K., Okazaki, K., Takahashi, H. et al. Molecular mapping of a gene conferring resistance to Aphanomyces root rot (black root) in sugar beet (Beta vulgaris L.). Euphytica 173, 409–418 (2010). https://doi.org/10.1007/s10681-010-0153-8

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  • DOI: https://doi.org/10.1007/s10681-010-0153-8

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