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Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection

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Abstract

Conventional methods for the selection of papaya pure lines are time-consuming. Procedures involving the use of molecular markers and the indirect selection of homozygous plants can reduce this time considerably. The objective of this study was to evaluate the informativeness of a microsatellite marker set when used in marker-assisted selection (MAS) for the development of new papaya lines. Eighty-three lines originating from two segregating F3 populations and from papaya germplasm were used for the molecular analysis of 27 microsatellite primers. Twenty polymorphic microsatellite primers were identified, allowing the identification of 86 alleles, with an average of 3.18 alleles per primer. The observed heterozygosity values were low for both the markers (0.00–0.29) and the individual lines (0.00–0.35). The inbreeding coefficient (f) ranged from 0.634 to 1.00. Eleven lines with f = 1.00 and 18 lines with f varying from 0.953 to 0.961 were identified. In addition, papaya lines showed high genetic diversity, which will certainly contribute to the development of new varieties. Our results show that the use of microsatellites in MAS is a quick and effective procedure for the development of papaya lines.

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Acknowledgments

This work was supported by Empresa Brasileira de Pesquisa Agropecuária, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb).

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Authors and Affiliations

  1. Embrapa Mandioca e Fruticultura Tropical, Cruz das Almas, BA, 44380-000, Brazil

    Eder Jorge de Oliveira, Vanusia Batista de Oliveira Amorim & Jorge Luiz Loyola Dantas

  2. Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil

    Aline dos Santos Silva, Lucas Ferraz dos Santos & Juliana Leles Costa

  3. Faculdade Maria Milza, Cruz das Almas, BA, Brazil

    Fabiana Moraes de Carvalho

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  1. Eder Jorge de Oliveira
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  2. Aline dos Santos Silva
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  3. Fabiana Moraes de Carvalho
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  4. Lucas Ferraz dos Santos
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  5. Juliana Leles Costa
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  6. Vanusia Batista de Oliveira Amorim
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  7. Jorge Luiz Loyola Dantas
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Correspondence to Eder Jorge de Oliveira.

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de Oliveira, E.J., dos Santos Silva, A., de Carvalho, F.M. et al. Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection. Euphytica 173, 279–287 (2010). https://doi.org/10.1007/s10681-010-0150-y

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  • DOI: https://doi.org/10.1007/s10681-010-0150-y

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