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Using molecular markers and field performance data to characterize the Pee Dee cotton germplasm resources

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Abstract

Knowledge of genetic relationships in crop breeding programs provides valuable information that can be used by plant breeders as a parental line selection tool. In Upland cotton (Gossypium hirsutum L.), the Pee Dee germplasm program represents one of the most historically significant Upland cotton breeding programs and is known as a key source of fiber quality genes for commercial cultivars. The foundation of the Pee Dee germplasm is known to represent an array of genetic diversity involving the hybridization of G. hirsutum L., G. barbadense L., and triple hybrid strains (G. arboreum L. × G. thurberi Todaro × G. hirsutum L.). In this study, we characterized genetic relationships within the Pee Dee germplasm collection using molecular marker and field performance data. Molecular marker and field performance data showed the Pee Dee germplasm collection still maintains useful amounts of genetic diversity. The methods described provide plant breeders of cotton and other crops a strategy to develop a parental line selection tool based on genotypic and phenotypic information. Cotton breeders can directly use the information provided to select specific Pee Dee germplasm parental line combinations based on genotypic (molecular marker) and phenotypic (field performance) information rather than relying on pedigree and phenotypic information alone.

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Abbreviations

AFIS:

Advanced fiber information system

HVI:

High volume instrument

UPGMA:

Unweighted pair group method with arithmetic average

SSR:

Simple sequence repeat

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Acknowledgments

This research project was supported by funding from CRIS No. 6657-21000-005-00D of the U.S. Department of Agriculture and a grant from Cotton Incorporated. Special thanks to Bobby Fisher and summer students for technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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  1. V. E. Williams

    Present address: Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, 29425, USA

Authors and Affiliations

  1. USDA-ARS Coastal Plains Soil, Water and Plant Research Center, 2611 W. Lucas St., Florence, SC, 29501, USA

    B. T. Campbell, V. E. Williams & W. Park

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  1. B. T. Campbell
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Correspondence to B. T. Campbell.

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Campbell, B.T., Williams, V.E. & Park, W. Using molecular markers and field performance data to characterize the Pee Dee cotton germplasm resources. Euphytica 169, 285–301 (2009). https://doi.org/10.1007/s10681-009-9917-4

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