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Genetic analysis of partial resistance to coffee leaf rust (Hemileia vastatrix Berk & Br.) introgressed into the cultivated Coffea arabica L. from the diploid C. canephora species

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Abstract

The objective of this study was to identify polymorphic molecular markers associated with partial resistance to coffee leaf rust, Hemileia vastarix. A segregating F 2 population derived from a cross between the susceptible Coffea arabica cv. Caturra and a C. canephora-introgressed Arabica line exhibiting high partial resistance was analyzed. Rust resistance measured as rust incidence (RI) and defoliation (DEF) was evaluated in field conditions in three consecutive years (2003–2005). During the 2003 season, which was characterized by favorable conditions for a rust epidemic, the F 2 plants exhibited different levels of resistance ranging from very susceptible (50.1% for DEF and 49.5% for RI) to highly partial resistance (9.1% for DEF and 3.7% for RI). Molecular analysis enabled identification of seven polymorphic markers (5 AFLP and 2 SSR) exhibiting significant association with partial resistance. Coexistence of resistance homozygous alleles (RR) at codominant SSR loci was correlated with high resistance. This study is the first attempt to develop PCR-based sequence specific markers linked to partial rust resistance in coffee.

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Acknowledgments

This research was supported by grants from the Minister of Agriculture and Rural Development of Colombia through a collaborative project between the Institut de Recherche pour le Développement (IRD, France) and the National Center of Coffee Research (CENICAFE, Colombia). We are grateful to JC Montoya and O Taborda for technical assistance. We also thank the anonymous reviewers for helpful comments and suggestions on the manuscript.

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Authors and Affiliations

  1. Centro Nacional de Investigaciones de Café, CENICAFE – FNC, Reserva Plan Alto, Km 4, Chinchiná (Caldas), AP 2427, Manizales, Colombia

    Juan Carlos Herrera P., Gabriel Alvarado A., Hernando A. Cortina G. & Gladys Romero G.

  2. IRD Institut de Recherche pour le Développement, UMR RPB, GeneTrop, 911, Av Agropolis, BP 64501, 34394, Montpellier Cedex 5, France

    Marie-Christine Combes & Philippe Lashermes

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  1. Juan Carlos Herrera P.
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  2. Gabriel Alvarado A.
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  3. Hernando A. Cortina G.
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  4. Marie-Christine Combes
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  6. Philippe Lashermes
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Correspondence to Juan Carlos Herrera P..

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Herrera P., J.C., Alvarado A., G., Cortina G., H.A. et al. Genetic analysis of partial resistance to coffee leaf rust (Hemileia vastatrix Berk & Br.) introgressed into the cultivated Coffea arabica L. from the diploid C. canephora species. Euphytica 167, 57–67 (2009). https://doi.org/10.1007/s10681-008-9860-9

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