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Production of interspecific hybrids between Lilium longiflorum and L. lophophorum var. linearifolium via ovule culture at early stage

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Abstract

Interspecific hybridization was carried out between Lilium longiflorum and L. lophophorum var. linearifolium by using the cut style method of pollination, as a contrast, intraspecific hybridization between L. longiflorum ‘Gelria’ and L. longiflorum was also made, but no mature seeds and offspring were obtained from the two combinations under in vivo condition. Ovules excised from each carpel 5–35 days after pollination (DAP) were cultured on B5 or half-strength B5 medium containing sucrose at different concentrations in vitro. In L. longiflorum × L. lophophorum var. linearifolium, only 1.17% of ovules excised at 10 DAP developed into seedlings, and in L. longiflorum ‘Gelria’ × L. longiflorum, only 0.99% of ovules excised at 25 DAP developed into seedlings; none of the ovules excised at other different DAP in the two cross combinations produced any seedlings. The results showed that interspecific hybridization had a more serious post-fertilization barrier than the intraspecific hybridization, and that a lower concentration (3%) of sucrose led to better embryo development and higher percentage of seedlings in ovule cultures. All hybrid seedlings obtained were successfully transplanted to soil and grew normally. The progenies investigated were identified as true hybrids based on inter-simple sequence repeat (ISSR) analysis.

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Abbreviations

BA:

6-Benzylaminopurine

CSM:

Cut style method

DAP:

Days after pollination

ISSR:

Inter-simple sequence repeat

NAA:

1-Naphthaleneacetic acid

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Acknowledgments

This work was supported by the “863” program (grant no. 2006AA100109) from the Ministry of Science and Technology of the People’s Republic of China. We thank all the colleagues in our laboratory for constructive discussion and technical assistance.

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Author notes

  1. Jie Wang

    Present address: Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, People’s Republic of China

Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Jie Wang, Li Huang, Man-zhu Bao & Guo-feng Liu

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  1. Jie Wang
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  2. Li Huang
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Correspondence to Guo-feng Liu.

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Wang, J., Huang, L., Bao, Mz. et al. Production of interspecific hybrids between Lilium longiflorum and L. lophophorum var. linearifolium via ovule culture at early stage. Euphytica 167, 45–55 (2009). https://doi.org/10.1007/s10681-008-9859-2

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