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Genome discrimination and chromosome pairing in the Hordeum chilense × Aegilops tauschii amphiploid

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Abstract

Tritordeum (X Tritordeum Ascherson et Graebner) is a synthetic amphiploid belonging to the Triticeae tribe, which resulted from crosses between Hordeum chilense and wheat. It presents useful agronomic traits that could be transferred to wheat, widening its genetic basis.

In situ hybridisation with total genomic DNA from H. chilense and cloned, repetitive DNA sequences (pTa71 and pAs1) probes were used to discriminate the parental origin of all chromosomes, to analyse the chromosome pairing and to identify the chromosomes in pollen mother cells (PMCs) at metaphase I of the tritordeum line HT251 (HchHchDD, 2n = 4x = 28).

The H. chilense total genomic DNA and the ribosomal sequence pTa71 probes, allowed the unequivocal discrimination of the 14 chromosomes of Hch genome-origin and the 14 chromosomes of D genome-origin.

Chromosome pairing analysis revealed meiotic irregularities such as reduced percentage of PMCs with complete homologous pairing, high frequency of univalents, most of H. chilense-origin and a reduced frequency of intragenomic multivalents from both genomes. The H. chilense genome revealed high meiotic instability.

After individual chromosome identification at metaphase I with the pAs1 probe, we found the occurrence of pairing between chromosomes of different homoeology groups. The possible interest of the tetraploid tritordeum in the improvement of other Triticeae species is also discussed.

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References

  • Cabrera, A. & A. Martín, 1991. Cytology and morphology of the amphiploid Hordeum chilense (4x) × Aegilops squarrosa (4x). Theor Appl Genet 81: 758–760.

    Article  Google Scholar 

  • Cabrera, A., A. Martín & F. Barro, 2002. In-situ comparative mapping (ISCM) of Glu-1 loci in Triticum and Hordeum. Chrom Res 10: 49–54.

    Article  PubMed  Google Scholar 

  • Chen, Q., R.L. Conner, A. Laroche & F. Ahmad, 2001. Molecular cytogenetic evidence for a high level of chromosome pairing among different genomes in Triticum aestivumThinopyrum intermedium hybrids. Theor Appl Genet 102: 847–852

    Article  Google Scholar 

  • Fernández, J.A., J. González, & N. Jouve, 1985. Meiotic pairing of the amphiploid Hordeum chilense × T. turgidum conv. durum studied by means of Giemsa C-Banding technique. Theor Appl Genet 70: 85–91.

    Google Scholar 

  • Fernández-Calvin, B., E. Benavente & J. Orellana, 1995. Meiotic pairing in wheat–rye derivatives detected by genomic in situ hybridisation and C-banding- a comparative analysis. Chromosoma 103: 554–558.

    PubMed  Google Scholar 

  • Gerlach, W.L. & J.R. Bedbrook, 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl Acids Res 7: 1869–1885.

    PubMed  Google Scholar 

  • Guedes-Pinto, H., V. Carnide & A.F. Silva, 1991. Triticale farm evaluation experiments in northern Portugal. In: Proceedings of the 2nd Symposium, Mexico D.F., CIMMYT, pp. 636–639.

  • Lima-Brito, J., H. Guedes-Pinto, G.E. Harrison & J.S. Heslop-Harrison, 1996. Chromosome identification and nuclear architecture in Triticale × Tritordeum F1 hybrids. J Exp Botany 47: 583–588.

    Google Scholar 

  • Martín, A. & E. Sánchez Monge-Laguna, 1982. Cytology and morphology of the amphiploid Hordeum chilense × Triticum turgidum conv. durum. Euphytica 31: 262–267.

    Google Scholar 

  • Martín, A., J.B. Alvarez, L.M. Martín, E. Barro & J. Ballesteros, 1999. The development of tritordeum: A novel cereal for food processing. J Cereal Sci 30: 85–95.

    Article  Google Scholar 

  • Martín, A., L.M. Martín, A. Cabrera, M.C. Ramirez, M.J. Gimenez, D. Rubiales, P. Hernandez & J. Ballesteros, 1998. The potential of Hordeum chilense in breeding Triticeae species. In: A.A. Jaradat (Ed.), Triticeae III, pp. 377–386, Science Publish, U.S.A.

    Google Scholar 

  • Martín, A., D. Rubiales, J.M. Rubio & A. Cabrera, 1995. Hybrids between Hordeum vulgare and tetra-, hexa- and octoploid tritordeums (amphiploid H. chilense × Triticum spp.). Hereditas 123: 175–182.

    Article  Google Scholar 

  • Miller, T.E., S.M. Reader, K.A. Purdie & I.P. King, 1994. Determination of the frequency of wheat–rye chromosome pairing in wheat × rye chromosome pairing in wheat × rye hybrids with and without chromosome 5B. Theor Appl Genet 89: 255–258

    Article  Google Scholar 

  • Mukai, Y., Y. Nakahara & M. Yamamoto, 1993. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridisation using total genomic and highly repeated DNA probes. Genome 36: 489–494.

    Google Scholar 

  • Pohler, W. & O. Schrader, 1990. Nearly total absence of homoeologous pairing in a hybrid between tetraploid Hordeum chilense and Triticum aestivum. Plant Breeding 104: 255–257.

    Google Scholar 

  • Rayburn, A.L. & B.S. Gill, 1986a. Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Molec Biol Rep 4(2): 102–109.

    Google Scholar 

  • Rayburn, A.L. & B.S. Gill, 1986b. Molecular identification of the D-genome chromosomes of wheat. J Hered 77: 253–255.

    Google Scholar 

  • Schwarzacher, T. & J.S. Heslop-Harrison, 2000. Practical in situ Hybridisation, Oxford BIOS, Scientific Publishers Ltd., U.K. 203 p.

    Google Scholar 

  • Schwarzacher, T., J.S. Heslop-Harrison, K. Anamthawat-Jónsson, R.A. Finch & M.D. Bennett, 1992. Parental genomes separation in reconstructions of somatic and pre-meiotic metaphases of Hordeum vulgare × H. bulbosum. J Cell Sci 101: 13–24.

    Google Scholar 

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Correspondence to José Lima-Brito.

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Carvalho, A., Guedes-Pinto, H., Mártín, A. et al. Genome discrimination and chromosome pairing in the Hordeum chilense × Aegilops tauschii amphiploid. Euphytica 144, 85–89 (2005). https://doi.org/10.1007/s10681-005-4616-2

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