Abstract
Oryza rufipogon (IRGC105491) is a wild relative of cultivated rice, it contains two favorable yield-enhancing genes (yld1.1 and yld2.1) on chromosomes 1 and 2, respectively, which are capable of improving the yield of hybrid rice by 18 and 17%, respectively. SSR markers RM9, RM24, RM5 and RM306 are flanking yld1.1, while RM166 and RM208 are mapped in the close region to yld2.1. These molecular markers tightly linked to the two yield-enhancing genes were used to screen the plants of backcross population between ‘9311’ (one of the top-performing parental lines in super hybrid rice seed production in China) and O. rufipogon. The results were as follows: (1) in BC2F1 population, the percentage of the individuals which contain both of the O. rufipogon alleles at marker loci RM166 and RM9 was 16.8%; (2) 1.5% individuals of total BC3F1 population have all the six linked markers (RM166, RM9, RM5, RM208, RM24, RM306); (3) in BC4F1 population, the percentage of the individuals which contain both of the two O. rufipogon alleles at marker loci RM166 and RM9 was 18.0%. Based on marker genotypes, the individuals, that contain multiple O. rufipogon markers, were selected and used for further backcross and self cross. Many ‘9311’-type lines with yield-enhancing genes and high yield potential were obtained. After three times self-crossing a stable improved ‘9311’ line was obtained. The results indicated that these molecular markers are feasible for marker-assisted selection (MAS) to screen rice individuals with high yield potential.
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Liang, F., Deng, Q., Wang, Y. et al. Molecular marker-assisted selection for yield-enhancing genes in the progeny of “9311×O. rufipogon” using SSR. Euphytica 139, 159–165 (2004). https://doi.org/10.1007/s10681-004-2560-1
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DOI: https://doi.org/10.1007/s10681-004-2560-1