Abstract
The impact of repeated applications of buprofezin and acephate, at concentrations ranging from 0.25 to 1.0 kg ha−1, on activities of cellulases, amylase, and invertase in unamended and nitrogen, phosphorous, and potassium (NPK) fertilizer-amended soil planted with cotton was studied. The nontarget effect of selected insecticides, when applied once, twice, or thrice on soil enzyme activities, was dose-dependent; the activities decreased with increasing concentrations of insecticides. However, there was a rapid decline in activities of enzymes after three repeated applications of insecticides in unamended or NPK-amended soil. Our data clearly suggest that insecticides must be applied judiciously in pest management in order to protect the enzymes largely implicated in soil fertility.
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References
Anderson, J. R. (1978). Pesticide effects on non-target soil microorganisms. In I. R. Hill & S. J. L. Write (Eds.), Pesticide microbiology (pp. 313–533). London: Academic.
Bielinska, E. J., & Pranagal, J. (2007). Enzymatic activity of soil contaminated with triazine herbicides. Polish Journal of Environmental Studies, 16, 295–300.
Casida, J., & Quistad, G. (2004). Organophosphate toxicology: safety aspects of non-acetyl cholinesterase secondary targets. Chemical Research in Toxicology, 17, 983–998.
Cole, M. A. (1977). Lead inhibition of enzyme synthesis in soil. Applied and Environmental Microbiology, 33, 262–268.
Cycon, M., & Piotrowska-Seget, Z. (2007). Effect of selected pesticides on soil microflora involved in organic matter and nitrogen transformations: pot experiment. Polish Journal of Ecology, 55, 207–220.
Gundi, V. A., Viswanath, B., Chandra, M. S., Kumar, V. N., & Reddy, B. R. (2007). Activities of cellulase and amylase in soils as influenced by insecticide interactions. Ecotoxicology and Environmental Safety, 68, 278–285.
Ismail, B. S., Yapp, K. F., & Omar, O. (1998). Effects of metsulfuron-methyl on amylase, urease and protease activities in two soils. Australian Journal of Soil Research, 36, 449–456.
Lethbridge, G., & Burns, R. G. (1976). Inhibition of soil urease by organophosphorous insecticides. Soil Biology and Biochemistry, 8, 99–102.
Lodhi, A., Malik, N. N., Mahmood, T., & Azam, F. (2000). Response of soil microflora, microbial biomass and some soil enzymes to baythroid (an insecticide). Pakistan Journal of Biological Sciences, 3, 868–871.
Mandic, L., Dukic, D., Govedarica, M., & Stanien Kovic, S. (1997). The effect of some insecticides on the number of amylolytic microorganisms and Azotobacter in apple nursery soil. Jugoslovensko Vocarstvo, 31, 177–184.
Megharaj, M. (2002) Heavy pesticide use lowers soil health. Kondinin Landcare. http://www.clw.csiro.au/publications/farming_ahead/2002/p3738_FA121_JAN02.pdf, 2002.
Mishra, P. C., & Pradhan, S. C. (1987). Seasonal variation in amylase, invertase, cellulase activity and carbon dioxide evolution in tropical protected grassland of Orissa, India, sprayed with carbaryl insecticide. Environmental Pollution, 43, 291–300.
Nannipieri, P. (1994). The potential use of soil enzymes as indicators of productivity, sustainability and pollution. In C. E. Pankhurst, B. M. Doube, V. V. S. R. Gupta, & P. R. Grace (Eds.), Soil biota: management in sustainable farming systems (pp. 238–244). Melbourne: CSIRO.
Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry, 153, 375–380.
Omar, S. A., & Abdel-Sater, M. A. (2001). Microbial populations and enzyme activities in soil treated with pesticides. Water, Air, and Soil Pollution, 127, 49–63.
Omar, S. A., Moharram, A. M., & Abd-Alla, M. H. (1993). Effects of an organophosphorous insecticide on the growth and cellulolytic activity of fungi. International Biodeterioration & Biodegrad, 31, 305–310.
Pancholy, S. K., & Rice, E. L. (1973). Soil enzymes in relation to old field succession: amylase, cellulase, invertase, dehydrogenase and urease. Soil Science Society of America Proceedings, 37, 47–50.
Ramakrishnan, B., Megharaj, M., Venkateswarlu, K., Naidu, R., & Sethunathan, N. (2010). The impacts of environmental pollutants on microalgae and cyanobacteria. Critical Reviews in Environmental Science and Technology, 40, 699–821.
Ramakrishnan, B., Megharaj, M., Venkateswarlu, K., Sethunathan, N., & Naidu, R. (2011). Mixtures of environmental pollutants: effects on microorganisms and their activities. Reviews of Environmental Contamination and Toxicology, 211, 63–120.
Rangaswamy, V., & Venkateswarlu, K. (1992). Activities of amylase and invertase as influenced by the applications of monocrotophos, quinalphos, cypermethrin and fenvalerate to groundnut soil. Chemosphere, 25, 525–530.
Rangaswamy, V., Reddy, B. R., & Venkateswarlu, K. (1994). Activities of dehydrogenase and protease in soil as influenced by monocrotophos, quinalphos, cypermethrin and fenvalerate. Agriculture, Ecosystems & Environment, 47, 319–326.
Ross, D. J., & Speir, T. W. (1985). Changes in biochemical activities of soil incubated with the nematicides and fenamiphos. Soil Biology and Biochemistry, 17, 123–125.
Ross, D. J., Speir, T. W., Cowling, J. C., & Whale, K. N. (1984). Influence of field applications of oxamyl and fenamiphos on biochemical activities of soil under pasture. New Zealand Journal of Science, 27, 247–254.
Sannino, F., & Gianfreda, L. (2001). Pesticide influence on soil enzymatic activities. Chemosphere, 45, 417–425.
Shittu, O.B., Akintokun, A.K., Akintokun, P.O., and Gbadebo, M.O. (2004) Effect of diazinon application on soil properties and soil microflora. Proceedings of International Conference and National Development, 25th–28th October. COLNAS Proc, pp. 68–74.
Singh, B., & Walker, A. (2006). Microbial degradation of organophosphorus compounds. FEMS Microbiology Reviews, 30, 428–471.
Singh, B. K., Allan, W., & Denus, J. W. (2002). Degradation of chloropyrifos, fenamiphos and chlorothalonil alone and in combination and their effects on soil microbial activity. Environmental Toxicology and Chemistry, 21, 2600–2605.
Tolan, V., & Ensari, Y. (2006). Effect of endosulfan on growth, α-amylase activity and plasmid amplification in Bacillus subtilis. Indian Journal of Biochemistry and Biophysics, 43, 123–126.
Tu, C. M. (1982). Influence of pesticides on activities of amylase, invertase and level of adenosine triphosphate in organic soil. Chemosphere, 2, 909–914.
Tu, C. M. (1988). Effect of selected pesticides on activities of invertase, amylase and microbial respiration in sandy soil. Chemosphere, 17, 159–163.
Tu, C. M. (1993). Effect of fungicides, captafol and chlorothalonil, on microbial and enzymatic activities in mineral soil. Journal of Environment Science and Health, 28B, 67–80.
Tu, C. M. (1995). Effect of five insecticides on microbial and enzymatic activities in sandy soil. Journal of Environment Science and Health, 30B, 289–306.
Voets, J. P., Meerschman, P., & Verstraete, W. (1974). Soil microbiological and biochemical effects of long term atrazine applications. Soil Biology and Biochemistry, 6, 149–152.
Wyszkowska, J., & Kucharski, J. (2004). Biochemical and physicochemical properties of soil contaminated with herbicide Triflurotex 250 EC. Polish Journal of Environmental Studies, 3, 223–231.
Acknowledgments
MN thanks Dr. S. Imthiyaz Ahamed (National P.G. College, Nandyal, India) for providing lab facilities and encouragement. We acknowledge the help from Dr. Sandra Rios (Unversidad de Los Logos, Centro Ceder, Chile) in the statistical analysis of the data.
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Raju, M.N., Venkateswarlu, K. Effect of repeated applications of buprofezin and acephate on soil cellulases, amylase, and invertase. Environ Monit Assess 186, 6319–6325 (2014). https://doi.org/10.1007/s10661-014-3856-9
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DOI: https://doi.org/10.1007/s10661-014-3856-9