Skip to main content
SpringerLink
Log in

Effects of pig manure containing copper and zinc on microbial community assessed via phospholipids in soils

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Pig manure (PM) is widely used as an organic fertilizer to increase yields of crops. Excessive application of compost containing relatively great concentrations of copper (Cu) and zinc (Zn) can change soil quality. To clarify the effects of different rates of application and to determine the optimal rate of fertilization, PM containing 1,115 mg Cu kg−1, dry mass (dm) and 1,497 mg Zn kg−1, dm was applied to alkaline soil at rates of 0, 11, 22, 44, 88, and 222 g PM kg−1, dm. Phospholipid fatty acids (PLFAs) were used to assess soil microbial community composition. Application of PM resulted in greater concentrations of total nitrogen (TN), NH4 +-N, NO3 −-N, total carbon (TC), soil organic matter (SOM) but lesser pH values. Soils with application rates of 88–222 g PM kg−1, dm had concentrations of total and EDTA-extractable Cu and Zn significantly greater than those in soil without PM, and concentrations of T-Cu and T-Zn in these amended soils exceeded maximum limits set by standards in china. Except in the soil with a rate of 11 g PM kg−1, dm, total bacterial and fungal PLFAs were directly proportional to rate of application of PM. Biomasses of bacteria and fungi were significantly greater in soils with application rates of 44–222 g PM kg−1, dm than in the soil without PM. SOM, TC and EDTA-Zn had the most direct influence on soil microbial communities. To improve fertility of soils and maintain quality of soil, rate of application should be 22–44 g PM kg−1 dm, soil containing Cu and Zn.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Al Chami, Z., Cavoski, I., Mondelli, D., & Miano, T. (2013). Effect of compost and manure amendments on zinc soil speciation, plant content, and translocation in an artificially contaminated soil. Environmental Science and Pollution Research, 20, 4766–4776.

    Article  Google Scholar 

  • Anderson, I. C., Parkin, P. I., & Campbell, C. D. (2008). DNA- and RNA-derived assessments of fungal community composition in soil amended with sewage sludge rich in cadmium, copper and zinc. Soil Biology and Biochemistry, 40, 2358–2365.

    Article  CAS  Google Scholar 

  • Angers, D. A., Chantigny, M. H., MacDonald, J. D., Rochette, P., & Cǒté, D. (2010). Differential retention of carbon, nitrogen and phosphorus in grassland soil profiles with long-term manure application. Nutrient Cycling in Agroecosystems, 86, 225–229.

    Article  Google Scholar 

  • Atafar, Z., Mesdaghinia, A., Nouri, J., Homaee, M., Yunesian, M., Ahmadimoghaddam, M., & Mahvi, A. H. (2010). Effect of fertilizer application on soil heavy metal concentration. Environmental Monitoring and Assessment, 160, 83–89.

    Article  CAS  Google Scholar 

  • Azarbad, H., Niklińska, M., van Gestel, C. A., van Straalen, N. M., Röling, W. F., & Laskowski, R. (2013). Microbial community structure and functioning along metal pollution gradients. Environmental Toxicology and Chemistry, 32, 1992–2002.

    Article  CAS  Google Scholar 

  • Baker, L. R., White, P. M., & Pierzynski, G. M. (2011). Changes in microbial properties after manure, lime, and bentonite application to a heavy metal-contaminated mine waste. Applied Soil Ecology, 48, 1–10.

    Article  Google Scholar 

  • Bhattacharyya, P., Tripathy, S., Chakrabarti, K., Chakraborty, A., & Banik, P. (2008). Fractionation and bioavailability of metals and their impacts on microbial properties in sewage irrigated soil. Chemosphere, 72, 543–550.

    Article  CAS  Google Scholar 

  • Bremner, J. M. (1965). Methods of soil analysis. Madison, WI: American Society of Agronomy.

    Google Scholar 

  • Cao, Y., Chang, Z., Wang, J., Ma, Y., & Fu, G. (2013). The fate of antagonistic microorganisms and antimicrobial substances during anaerobic digestion of pig and dairy manure. Bioresource Technology, 136, 664–671.

    Article  CAS  Google Scholar 

  • Chen, T. B., Zheng, Y. M., Chen, H., & Zheng, G. D. (2004). Background concentrations of soil heavy metals in Beijing. Environmental Sciences, 25, 117–112.

    Google Scholar 

  • Chodak, M., Gołębiewski, M., Morawska-Płoskonka, J., Kuduk, K., & Niklińska, M. (2013). Diversity of microorganisms from forest soils differently polluted with heavy metals. Applied Soil Ecology, 64, 7–14.

    Article  Google Scholar 

  • Cook, B. D., & Allan, D. L. (1992). Dissolved organic carbon in old field soils: total amounts as a measure of available resources for soil mineralization. Soil Biology & Biochemistry, 24, 585–594.

    Article  CAS  Google Scholar 

  • de Vries, F. T., Hoffland, E., van Eekeren, N., Brussaard, L., & Bloem, J. (2006). Fungal/bacterial ratios in grasslands with contrasting nitrogen management. Soil Biology and Biochemistry, 38, 2092–2103.

    Article  Google Scholar 

  • Duan, G., Zhang, H., Liu, Y., Jia, Y., Hu, Y., & Cheng, W. (2012). Long-term fertilization with pig-biogas residues results in heavy metal accumulation in paddy field and rice grains in Jiaxing of China. Soil Science and Plant Nutrition, 58, 637–646.

    Article  CAS  Google Scholar 

  • Duarte, S., Pascoal, C., Alves, A., Correia, A., & Cassio, F. (2008). Copper and zinc mixtures induce shifts in microbial communities and reduce leaf litter decomposition in streams. Freshwater Biology, 53, 91–101.

    CAS  Google Scholar 

  • Fan, J., Ding, W., & Ziadi, N. (2013). Thirty-year manuring and fertilization effects on heavy metals in black soil and soil aggregates in northeastern China. Communications in Soil Science and Plant Analysis, 44, 1224–1241.

    Article  CAS  Google Scholar 

  • Fernández-Calviño, D., Martín, A., Arias-Estévez, M., Bååth, E., & Díaz-Raviña, M. (2010). Microbial community structure of vineyard soils with different pH and copper content. Applied Soil Ecology, 46, 276–282.

    Article  Google Scholar 

  • Frostegård, A., & Bååth, E. (1996). The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biology and Fertility of Soils, 22, 59–65.

    Article  Google Scholar 

  • Hammesfahr, U., Heuer, H., Manzke, B., Smalla, K., & Thiele-Bruhn, S. (2008). Impact of the antibiotic sulfadiazine and pig manure on the microbial community structure in agricultural soils. Soil Biology and Biochemistry, 40, 1583–1591.

    Article  CAS  Google Scholar 

  • Hinojosa, M. B., Garcia-Ruiz, R., & Carreira, J. A. (2010). Utilizing microbial community structure and function to evaluate the health of heavy metal polluted soils. Soil Heavy Metals, 19, 185–224.

    Article  CAS  Google Scholar 

  • Huang, Z. P., Xu, B., & Tu, D. Y. (2007). Organic substrate change in the swine manure-applied vegetable soils. Journal of Anhui Agricultural University, 34, 262–264.

    CAS  Google Scholar 

  • Huang, D. F., Li, W. H., & Wang, L. M. (2014). Study on water and fertilizer managements and evaluation of nitrogen and phosphorus non-point source pollution from paddy field. Image Processing for Cinema, 4, 335.

    Google Scholar 

  • Jacela, J. Y., DeRouchey, J. M., Tokach, M. D., Goodband, R. D., Nelssen, J. L., & Renter, D. G. (2012). Feed additives for swine: fact sheets—high dietary levels of copper and zinc for young pigs, and phytase. Journal of Swine Health and Production, 18, 87–91.

    Google Scholar 

  • Krishnamurti, G. S., Subashchandrabose, S. R., Megharaj, M., & Naidu, R. (2013). Assessment of bioavailability of heavy metal pollutants using soil isolates of Chlorella sp. Environmental Science and Pollution Research, 1–7.

  • Lee, C. H., Wu, M. Y., Asio, V. B., & Chen, Z. S. (2006). Using a soil quality index to assess the effects of applying swine manure compost on soil quality under a crop rotation system in Taiwan. Soil Science, 171, 210–222.

    Article  CAS  Google Scholar 

  • Li, G. C., Wang, Y. P., & Chang, J. M. (1987). Publication by the Environmental Protection Administration (EPA) of Taiwan. Taipei: Taiwan. Heavy metal concentrations in soils of Taiwan.

    Google Scholar 

  • Lichtfouse, E. (2009). Organic farming, pest control and remediation of soil pollutant. Dijon, France: Springer.

  • Mackie, K., Müller, T., Zikeli, S., & Kandeler, E. (2013). Long-term copper application in an organic vineyard modifies spatial distribution of soil micro-organisms. Soil Biology and Biochemistry, 65, 245–253.

    Article  CAS  Google Scholar 

  • Meng, J., Wang, L., Liu, X., Wu, J., Brookes, P. C., & Xu, J. (2013). Physicochemical properties of biochar produced from aerobically composted swine manure and its potential use as an environmental amendment. Bioresource Technology, 142, 641–646.

    Article  CAS  Google Scholar 

  • Park, J. H., Lamb, D., Paneerselvam, P., Choppala, G., Bolan, N., & Chungd, J. W. (2010). Role of organic amendments on enhanced bioremediation of heavy metal(loid) contaminated soils. Journal of Hazardous Materials, 185, 549–574.

    Article  Google Scholar 

  • Rees, H., Chow, L., Zebarth, B., Xing, Z., Toner, P., & Lavoie, J. (2013). Impact of supplemental poultry manure application on potato yield and soil properties on a loam soil in north western New Brunswick. Canadian Journal of Soil Science, 94, 1–17.

    Google Scholar 

  • Shi, J. C., Yu, X. L., Zhang, M. K., Lu, S. G., Wu, W. H., Wu, J. J., & Xu, J. M. (2011). Potential risks of copper, zinc, and cadmium pollution due to pig manure application in a soil–rice system under intensive farming: a case study of Nanhu, China. Journal of Environmental Quality, 40, 1695–1704.

    Article  CAS  Google Scholar 

  • Thu, T. D., Jusselme, D. M., Lata, J. C., Van Nguyen, B., & Jouquet, P. (2013). The earthworm species Metaphire posthuma modulates the effect of organic amendments (compost vs. vermicompost from buffalo manure) on soil microbial properties. A laboratory experiment. European Journal of Soil Biology, 59, 15–21.

  • Wakelin, S. A., Chu, G. X., Broos, K., Clarke, K. R., Liang, Y. C., & McLaughlin, M. J. (2010). Structural and functional response of soil microbiota to addition of plant substrate are moderated by soil Cu levels. Biology and Fertility of Soils, 46, 333–342.

    Article  CAS  Google Scholar 

  • Wei, F. S., Chen, J. S., Wu, Y. Y., & Zheng, C. J. (1991). Study on the background contents on 61 elements of soil in China. Environmental Sciences, 12, 12–20.

    CAS  Google Scholar 

  • Wu, L. H., Tan, C. Y., Liu, L., Zhu, P., Peng, C., Luo, Y. M., & Christie, P. (2012). Cadmium bioavailability in surface soils receiving long-term applications of inorganic fertilizers and pig manure. Geoderma, 173, 224–230.

    Article  Google Scholar 

  • Xiong, X., Li, Y., Li, W., Lin, C., Han, W., & Yang, M. (2010). Copper content in animal manures and potential risk of soil copper pollution with animal manure use in agriculture. Resources, Conservation and Recycling, 54, 985–990.

    Article  Google Scholar 

  • Zelles, L. (1999). Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review. Biology and Fertility of Soils, 29, 111–129.

    Article  CAS  Google Scholar 

  • Zhang, Q. C., Shamsi, I. H., Xu, D. T., Wang, G. H., Lin, X. Y., Jilani, G., Hussainc, N., & Chaudhry, A. N. (2012). Chemical fertilizer and organic manure inputs in soil exhibit a vice versa pattern of microbial community structure. Applied Soil Ecology, 57, 1–8.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Natural Science Foundation of China (Nos. 41271502 and C031001), Strategic Priority Research Program of the Chinese Academy of Sciences, Grant No. XDB03030504, the Special Program for Basic Science and Technology under Grant No. 2013FY111100. Portions of the research were supported by a Discovery Grant from the National Science and Engineering Research Council of Canada (Project No. 6807. Prof. Giesy’s participation in the project was supported as an at-large Chair Professorship from the City University of Hong Kong and by an "Area of Excellence" Grant (AoE P-04/04) from the Hong Kong Grants Council, the Einstein Professor Program of the Chinese Academy of Sciences.

Author information

Authors and Affiliations

  1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Yan Zhang, Wei Luo, Junmei Jia, Peiru Kong & Yonglong Lu

  2. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China

    Yan Zhang

  3. University of Chinese Academy of Science, Beijing, 100049, China

    Yan Zhang, Junmei Jia & Peiru Kong

  4. College of Forestry, Beijing Forestry University, Beijing, 100083, China

    Xiaojuan Tong, Liqiong Xie & Fulong Ma

  5. Department of Veterinary Biomedical Sciences and Toxicology Center, University of Saskatchewan, Saskatoon, SK, Canada

    John P. Giesy

  6. Department of Zoology, National Food Safety and Toxicology Center and Center for Integrative Toxicology, Michigan State University, East Lansing, MI, 48824, USA

    John P. Giesy

Authors
  1. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junmei Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peiru Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaojuan Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yonglong Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Liqiong Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fulong Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. John P. Giesy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei Luo.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Luo, W., Jia, J. et al. Effects of pig manure containing copper and zinc on microbial community assessed via phospholipids in soils. Environ Monit Assess 186, 5297–5306 (2014). https://doi.org/10.1007/s10661-014-3778-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10661-014-3778-6

Keywords

Search

Navigation