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Monitoring health implications of pesticide exposure in factory workers in Pakistan

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Abstract

The study aimed to determine the hazardous health effects of pesticides exposure in the factory workers by measuring plasma cholinesterase (PChE), pesticides residues, and renal and hepatic biochemical markers. In addition, we also assessed the knowledge, attitudes, and safety practices adopted by the industrial workers. The study was conducted in three different sizes of factories located in Lahore (large), Multan (medium), and Karachi (small) in Pakistan. Total 238 adult males consisting of 184 pesticide industrial workers (exposed group) from large-sized (67), medium-sized (61), small-sized (56) industrial formulation factories, and 54 controls (unexposed) were included in the study. All the participants were male of aged 18 to 58 years. PChE levels were estimated by Ellmann’s method. Plasma pesticides residue analysis was performed by using reverse phase C-18 on high-performance liquid chromatograph and GC with NPD detector. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, urea, and gamma glutamyltransferase (GGT) were measured on Selectra E auto analyzer. Plasma and C-reactive protein was analyzed by Immulite 1000. The results revealed a significant decrease in plasma post exposure PChE levels (<30%) as compared to baseline in the workers of small (29%) and medium (8%) industrial units (p < 0.001). Plasma cypermethrin, endosulfan, imidacloprid, thiodicarb, carbofuran, and methamidophos levels were found to be higher than allowable daily intake. Serum AST, ALT, creatinine GGT, malondialdehyde, total antioxidant, and CRP were significantly raised among the workers of small and medium pesticide formulation factories as compared to large industrial unit and controls (p < 0.001). The study demonstrated that unsafe practices among small- and medium-sized pesticides industrial workers cause significant increase in pesticide exposure, oxidative stress, and derangement of hepatic and renal function.

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References

  • Australian Government, Department of Health Sciences and Ageing, Office of Chemical Safety (2008). Acceptable daily intakes for agricultural and veterinary chemicals. Woden: Office of Chemical Safety, Australian Government. http://www.health.gov.au/internet/main/publishing.nsf/Content/ocs-adi-list.htm

  • Azmi, M. A., Naqvi, S. N., Azmi, M. A., & Aslam, M. (2006). Effect of pesticide residues on health and different enzyme levels in the blood of farm workers from Gadap (rural area) Karachi-Pakistan. Chemosphere, 64(10), 1739–1744.

    Article  CAS  Google Scholar 

  • Bergmeyer, H. U., Hereder, M., & Rej, R. (1998). Approved recommendation 1985 on IFCC method for the measurement of catalytic concentration of enzymes part 2. IFCC method for aspartate aminotransferase. Journal of Clinical Chemistry and Clinical Biochemistry, 24, 49.

    Google Scholar 

  • Cabello, G., Valenzuela, M., Vilaxa, A., Durán, V., Rudolph, I., Hrepic, N., et al. (2001). A rat mammary tumor model induced by the organophosphorus pesticides parathion and malathion, possibly through acetylcholinesterase inhibition. Environmental Health Perspectives, 109(5), 471–479.

    Article  CAS  Google Scholar 

  • Catharina, W., Matthew, K., Anders, A., Rob, M., Jai-Dong, M., Linda, R., et al. (2002). Long-term neurobehavioral effects of mild poisonings with organophosphate and n-methyl carbamate pesticides among banana workers. International Journal of Occupational and Environmental Health, 8(1), 27–34.

    Google Scholar 

  • Coronado, G. D., Thompson, B., Strong, L., Griffith, W. C., & Islas, I. (2004). Agricultural task and exposure to organophosphate pesticides among farm workers. Environmental Health Prospective, 112(2), 142–147.

    Article  CAS  Google Scholar 

  • Daniel, V., Huber, W., & Baver, K. (2002). Association of dichlorodiphenyl trichloroethne (DDT) 4.4. and DDE 4.4 blood levels with plasma IL.4 Arch. Environmental Health, 57(6), 541–547.

    Article  CAS  Google Scholar 

  • Dasgupta, S., Meisner, C., Wheeler, D., Xuyen, K., & Thi, L. N. (2007). Pesticide poisoning of farm workers – implications of blood test results from Vietnam. International Journal of Hygiene and Environmental Health, 210(2), 121–132.

    Article  CAS  Google Scholar 

  • Doumas, B. T., Watson, W. A., & Bigg, H. G. (1997). Albumin standards and the measurement of Plasma albumin with bromocresol green. Clin PChEm Acta, 31, 87–96.

    Article  Google Scholar 

  • Economic Survey of Pakistan (2008–9). Finance Division, Government of Pakistan, Islamabad. http://finance.gov.pk/survey/survey_2008–09/02-Agriculture.

  • El-Demerdash, F. M., Yousef, M. I., & Elagamy, E. I. (2001). Influence of paraquat, glyphosate, and cadmium on the activity of some serum enzymes and protein electrophoretic behavior (in vitro). Journal of Environmental Science and Health B, 36(1), 29–42.

    Article  CAS  Google Scholar 

  • Ellmann, G. L., Courney, K. D., Andres, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7, 75–88.

    Article  Google Scholar 

  • Gavino, V. C., Miller, J. S., Ikharebha, S. O., Milo, G. E. & Cornwall, D. G. (1981). Effects of polyunsaturated fattyacids and antioxidants on lipid peroxidation in tissue cultures. Journal of Lipid Research, 22, 763–769.

    CAS  Google Scholar 

  • Goel, A., Chanuhan, D. P., & Dhawan, D. K. (2000). Protective effects of zinc in chlorpyrifos induced hepatoxicity; a biochemical and trace element study. Biological Trace Element Research, 74, 171–183.

    Article  CAS  Google Scholar 

  • Hernández, A., Gómez, M. A., Pena, G., Gil, F., Rodrigo, L., Villanueva, E., et al. (2004). Effect of long-term exposure to pesticides on plasma esterases from plastic greenhouse workers. Journal of Toxicology and Environmental Health A, 67(14), 1095–1108.

    Article  CAS  Google Scholar 

  • Javed, A. B., Khan, Q. M., Haq, M. A., Khalid, A. M. & Nasim, A. (2006). Cytogenetic analysis of Pakistani individual’s occupationally exposed to pesticides in a pesticide production industry. Mutagenesis, 21(2), 143–148.

    Article  Google Scholar 

  • Kingsley, G. R. (1942). The direct Biuret method for the determination of serum proteinsas applied to photoelectric and visual colorimetry. Journal of Laboratory and Clinical Medicine, 27, 840–845.

    CAS  Google Scholar 

  • Merhi, M., Raynal, H., Cahuzac, E., Vinson, F., Cravedi, J. P., & Gamet-Payrastre, L. (2007). Occupational exposure to pesticides and risk of hematopoietic cancers: Meta-analysis of case-control studies. Cancer Causes Control, 18(10), 1209–1226.

    Article  CAS  Google Scholar 

  • Miller, N. J., Rice-Evans, C., Davies, M. J., Copinathan, V., & Milner, A. (1993). A novel method for measurement antioxidant capacity and its application to monitoring the antioxidant status in prematureneonates. Clinical Science, 84, 407–412.

    CAS  Google Scholar 

  • Misra, U. K., Nag, D., Dhushan, V., & Ray, P. K. (1985). Clinical and biochemical changes in chronically exposed organophosphate workers. Toxicology Letters, 244, 187–193.

    Article  Google Scholar 

  • Murphy, H. H., Haon, N. P., Matteson, P. & Morales, A. L. C. (2002). Farmers self surveillance of pesticide poisoning: A twelve month pilot in Northern Vietnam. International Journal of Occupational and Environmental Health, 8(3), 201–221.

    Google Scholar 

  • Ohayo-Mitoko, G. J. A., Kromhout, H., Simwa, J. M., Boleij, J. S. M., & Heederik, D. (2000). Self reported symptoms and inhibition of acetylcholinesterase activity among Kenyan agricultural workers. Occupational and Environmental Medicine, 57, 195–200.

    Article  CAS  Google Scholar 

  • Peres, F., Moreire, C. J., Rodrigues, M. K., & Claudio, L. (2006). Risk perception and communication regarding pesticide use in rural work: A case study in Rio de Janeiro State, Brazil. International Journal of Occupational and Environmental Health, 12(4), 400–407.

    Google Scholar 

  • Perry, B. W., Doumas, B. T., & Bayse, D. D. (1983). A candidate reference method for determination of bilirubin in plasma: Test for transferability. Clinical Chemistry, 29, 297–301.

    CAS  Google Scholar 

  • Ranjbar, A., Pasalar, P., & Abdollahi, M. (2002). Induction of oxidative stress and acetylchol inesterase inhibition in organophosphorous pesticide manufacturing workers. Human & Experimental Toxicology, 21(4), 179–182.

    Article  CAS  Google Scholar 

  • Sampson, E. J., & Baird, M. A. (1979). Chemical inhibition used in a Kinetic Urease/ glutamate dehhydrogenase method for urea in serum. Clinical Chemistry, 25, 1721–1729.

    CAS  Google Scholar 

  • Sanborn, M., Kerr, K. J., Sanin, L. H., Cole, D. C., Bassil, K. L., & Vakil, C. (2007). Non-cancer health effects of pesticides. Systematic review and implications for family doctors. Canadian Family Physician, 53, 1712–1720.

    CAS  Google Scholar 

  • Schumann, G., Bonora, R., Ceriotti, F., Clerc-Renaud, P., Ferard, G., Ferrero, C. A., et al. (2002). IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Reference procedure for the measurement of catalytic concentration of gamma glutamyltransferase. Chemistry and Laboratory Medicine, 0, 734–738.

    Article  CAS  Google Scholar 

  • Spencer, K. (1986). Analytical reviews in clinical biochemistry: The estimation of creatinine. Annals of Clinical Biochemistry, 23, 1–25.

    CAS  Google Scholar 

  • Tariq, M. I., Afzal, S., Hussain, I., & Sultana, N. (2007). Pesticides exposure in Pakistan: A review. Environment International, 33, 1107–1122.

    Article  CAS  Google Scholar 

  • US-EPA (1996). Method 3620B: Florisil cleanup. Washington, D.C.: U.S. Environmental Protection Agency.

    Google Scholar 

  • Wesseling, C., McConnell, R., Partanen, T., & Hogstedt, C. (1997). Agricultural pesticide use in developing countries: Health effects and research needs. International Journal of Health Services, 27(2), 273–308.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Pathology, Army Medical College, National University of Sciences and Technology, Rawalpindi, Pakistan

    Dilshad Ahmed Khan, Wajiha Mahjabeen & Tatheer A. Naqvi

  2. School of Civil and Environmental Engineering, National University of Sciences and Technology, Rawalpindi, Pakistan

    Imran Hashmi

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  1. Dilshad Ahmed Khan
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  2. Imran Hashmi
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  3. Wajiha Mahjabeen
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  4. Tatheer A. Naqvi
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Correspondence to Imran Hashmi.

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Khan, D.A., Hashmi, I., Mahjabeen, W. et al. Monitoring health implications of pesticide exposure in factory workers in Pakistan. Environ Monit Assess 168, 231–240 (2010). https://doi.org/10.1007/s10661-009-1107-2

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