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Determination of 7-ethoxyresorufin-o-deethylase (EROD) induction in leaping mullet (Liza saliens) from the highly contaminated Aliaga Bay, Turkey

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Abstract

Pollution of the aquatic environment is a global concern owing to the devastating effects of contaminants whose levels are growing at an alarming rate, and it has become a major threat for marine organisms, as well as to humans as consumers. This study has been carried out on leaping mullet obtained from Aliaga Bay, which is located on the west coast of the Aegean Sea near Izmir and hosts the world’s fifth largest shipyard, plus a broad range of industrial activities, including an oil refinery and a paper factory. The waste from these industries, combined with municipal sewer discharges, is the main cause of pollution in this region. There is no national documentation or research on the determination of pollution resulting from the industrial activities in this area. In the present study, the degree of induction of CYP4501A-associated 7-ethoxyresorufin O-deethylase (EROD) activity and immunochemical detection of CYP1A1 in the liver of leaping mullet (Lisa saliens) were used as biomarkers for the assessment of polycyclic aromatic hydrocarbon (PAH)-type organic pollutants in Aliaga Bay. Mullet caught from different locations of the bay had approximately 52 times more EROD activity than the feral fish sampled from a clean reference site near Foca, Izmir. The results of this study indicate that Aliaga Bay is highly contaminated with PAH-type organic pollutants.

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Authors and Affiliations

  1. Faculty of Arts & Sciences, Department of Biology, Pamukkale University, Kinikli Campus, 20017 Kinikli, Denizli, Turkey

    Alaattin Sen & Begum Tutuncu

  2. Faculty of Pharmacy, Department of Toxicology, Gazi University, 06330, Hipodrom, Ankara, Turkey

    Onur Kenan Ulutas & Ismet Cok

  3. Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, 06330, Hipodrom, Ankara, Turkey

    Nusret Ertas

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  1. Alaattin Sen
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  2. Onur Kenan Ulutas
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  3. Begum Tutuncu
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  4. Nusret Ertas
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  5. Ismet Cok
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Correspondence to Ismet Cok.

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Sen, A., Ulutas, O.K., Tutuncu, B. et al. Determination of 7-ethoxyresorufin-o-deethylase (EROD) induction in leaping mullet (Liza saliens) from the highly contaminated Aliaga Bay, Turkey. Environ Monit Assess 165, 87–96 (2010). https://doi.org/10.1007/s10661-009-0928-3

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