Abstract
The aim of this study was to identify possible relationships between biochemical- superoxide dismutase (SOD), glutathione peroxidase (GPx), thiobarbituric acid reactive substances (TBARS) of mussels (Mytilus galloprovincialis) and chemical (Hg, Cd, Pb, Cr, Cu, Zn, Mn and Fe) contaminants from relatively clean area (Middle Bay) and heavily polluted area (Inner Bay) of the Izmir Bay. Sampling of mussels was performed in the beginning of May 2004. Mussel digestive glands have been used for biochemical assays. Trace metal content was determined by atomic absorption spectrophotometer using standard procedures. Metal contents in mussels collected at polluted site increased compared to the middle part of the bay. While there was a positive correlation between metals and SOD enzyme activity, there was a negative correlation between metals and GPx enzyme activity. An increase was observed in SOD enzyme activity and a decrease of GPx enzyme activity at the inner part of the bay.
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Arinc, E., & Sen, A. (1999). Hepatic cytochrome P4501A and 7-ethoxyrcsorufin O-deethlase induction in mullet and common sole as an indicator of toxic organic pollution in Izmir Bay, Turkey. Marine Environmental Research, 48(2), 147–160.
Arinc, E., Sen, A., & Bozcaarmutlu, A. (2000). Cytochrome P4501A and associated mixed-function oxidase induction in fish as a biomarker for toxic carcinogenic pollutants in the aquatic environment. Pure Applied Chemistry, 2(6), 985–994.
Blum, J., & Fridovich, I. (1985). Inactivation of glutathione peroxidase by superoxide radical. Archives of Biochemistry and Biophysiology, 240, 500–508.
Bozcaarmutlu, A., & Arinc, E. (2004) Inhibitory effects of divalent metal ions on liver microsomal 7-ethoxyresorufin O-deethlase (EROD) activity of leaping mullet. Marine Environmental Research, 58(2–5), 521–524.
Carrillo, M. C., Kanai, S., Nokubo, M., & Kitani, K. (1991) (–) deprenyl induces activities of both superoxide dismutase and catalase but not of glutathione peroxidase in the striatum of young male rats. Life Science, 48, 517–521.
Company, R., Serafim, A., Bebianno, M. J., Cosson, R., Shillito, B., & Fiala-Medioni, A. (2004). Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus. Marine Environmental Research, 58, 377–381.
Da Ros, L., Nasci, C., Marigomez, I., Soto, M. (2000). Biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, Mytilus galloprovincialis, in Venice Lagoon, Italy. Marine Environmental Research, 50(1–5), 417–423.
De Almeida, E. A., Miyamoto, S., Bainy, A. C., De Medeiros, M. H., & Di Mascio, P. (2004). Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals. Marine Pollution Bulletin, 49, 386–392.
Doyotte, A., Cossu, C., Jacquin, M. C., Babut, M., & Vasseur, P. (1997). Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus. Aquatic Toxicology, 39, 93–110.
IMST. MEDPOL II. (1995). Long-term scientific monitoring and measurement project in the Aegean Sea. Technical Final Report, Institute of Marine Sciences & Technology, Izmir, Turkey.
IMST. MEDPOL II. (1997). Long-term scientific monitoring and measurement project in the Aegean Sea. Technical Final Report, Institute of Marine Sciences & Technology, Izmir, Turkey.
Kucuksezgin, F. (2001). Distribution of heavy metals in the surficial sediments of Izmir Bay (Turkey). Toxicological and Environmental Chemistry, 80(3–4), 203–207.
Kucuksezgin, F., Kontas, A., Altay, O., Uluturhan, E., & Darilmaz, E., (2006). Assessment of marine pollution in Izmir Bay: Nutrient, heavy metal and total hydrocarbon concentrations. Environment International, 32, 41–51.
Kucuksezgin, F., Uluturhan, E., Kontas, A., & Altay, O. (2002). Trace metal concentrations in edible fishes from Izmir Bay, Eastern Aegean. Marine Pollution Bulletin, 44, 827–832.
Manduzio, H., Monsinjon, T., Rocher, B., Leboulenger, F., & Galap, C. (2003). Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis. Aquatic Toxicology, 64, 73–83.
Moukrim, A., El Hamidi, F., Lagbouri, A., Kaaya, A., Zekhnini, A., Bouhaimi, A., et al. (2004). Study of Donax trunculus as a sentinel species for environmental monitoring of sandy beaches on Moroccan coasts. Bulletin of Environmental Contamination and Toxicology, 73, 674–681.
Nasci, C., Da Ros, L., Campesan, G., & Fossato, V.U. (1998). Assessment of the impact of chemical pollutants on mussel, Mytilus galloprovincialis, from the Venice Lagoon, Italy. Marine Environmental Research, 46(1–5), 279–282.
Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory of Clinical and Medical, 70, 158–169.
Regoli, F., & Principato, G. (1995). Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: Implications for the use of biochemical biomarkers. Aquatic Toxicology, 31, 143–164.
Rehncrona, S., Smith, D. S., Akesson, B., Westerberg, E., & Siesjo, B. K. (1980). Peroxidative changes in brain cortical fatty acids and phospholipids, as characterized during Fe2+- and ascorbic acid-stimulated lipid peroxidation in vitro. Journal of Neurochemistry, 34, 1630–1638.
Rodriguez-Ariza, A., Martinez-Lara, E., Pascual, P., Pedrajas, J. R., Dorado, G., Toribio, F., et al. (1993). Biochemical and genetic indices of marine pollution in Spanish littoral. Science of the Total Environment, 134, 109–116.
Thomas, P., & Wofford, H. W. (1993). Effects of cadmium and Aroclor 1254 on lipid peroxidation, glutathione peroxidase activity, and selected antioxidants in Atlantic croaker tissues. Aquatic Toxicology, 27, 159–178.
Torres, M. A., Testa, C. P., Gaspari, C., Masutti, M. B., Panitz, C. M. N., Curi-Pedrosa, R., et al. (2002). Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island, Brazil. Marine Pollution Bulletin, 44, 923–932.
UNEP. (1982). Reference methods for marine pollution studies, 14.
UNEP. (1984). Determination of total Cd, Zn, Pb and Cu in selected marine organisms by flameless AAS. Reference Methods for Marine Pollution Studies, 11.
UNEP. GESAMP. (1985). Cadmium, lead and tin in the marine environment. UNEP Regional Seas Reports and Studies, 56.
Walker, C. H. (1995). Biochemical biomarkers in ecotoxicology: Some recent developments. Science of the Total Environment, 171, 189–195.
Watanabe, N., Kamei, S., Ohkubo, A., Yamanaka, M., Ohsaawa, S., Makino, K., et al. (1986). Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer. Clinical Chemistry, 32, 1551–1554.
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Kucuksezgin, F., Kayatekin, B.M., Uluturhan, E. et al. Preliminary investigation of sensitive biomarkers of trace metal pollution in mussel (Mytilus galloprovincialis) from Izmir Bay (Turkey). Environ Monit Assess 141, 339–345 (2008). https://doi.org/10.1007/s10661-007-9900-2
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DOI: https://doi.org/10.1007/s10661-007-9900-2