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Morphological and molecular characterisation of Trichodorus hellalae n. sp. (Nematoda: Trichodoridae) from Norway

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Abstract

A new stubby-root nematode belonging to the Trichodorus sparsus complex was found in association with serious damage to Hill’s Yew hedges (Taxus x media ´Hillii´) in Oslo in 2017, characterised by chlorosis, wilting and loss of needles. T. hellalae n. sp. is about 800 μm long with medium-sized onchiostyle (55 μm, average), characterized in male by two ventromedian cervical papillae located beyond the onchiostyle region and with the secretory excretory pore (SE-pore) in between, in most type specimens, three ventromedian precloacal supplements with the posteriormost one opposite the anterior end of spicule manubrium and spicules 40 μm long (average) with widened manubrium, gradually tapered to a narrower blade without ornamentation of striae or bristles, but showing a minor indentation at level of posterior border of capsule of suspensor muscles. Gubernaculum with thickened keel-like posterior end and a thickened refractive anterior border. Females are characterised by a short pear-shaped vagina, less than 1/3rd of corresponding body width and very small rounded triangular vaginal sclerotized pieces in longitudinal optical section and vulva pore-like in ventral view; on each body side one sublateral body pore at about 3.5 body width anterior to vulva and one postadvulvar body pore. According to D2-D3 analyses, the Trichodorus hellalae n. sp. sequences are embedded in a maximally supported clade with several T. variabilis lineages. However, morphological and molecular species delimitation both support Trichodorus hellalae n. sp. as being a new species. Therefore, T. variabilis now appears to in fact consists of several cryptic species.

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Acknowledgements

The authors would like to thank Marjolein Couvreur for her help with preparing the samples for sequencing and the lay-out of the photo plate.

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Authors and Affiliations

  1. Department of Biology, Nematology Research Unit, Ghent University, 9000, Ghent, Belgium

    Wilfrida Decraemer & Wim Bert

  2. Christer Magnusson, Norwegian Institute of Bioeconomy Research (NIBIO), PO. Box 115, NO-1431, Aas, Norway

    Christer Magnusson

  3. Norwegian University of Life Sciences (NMBU), PO. Box 5003, NO-1433, Aas, Norway

    Christer Magnusson

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  1. Wilfrida Decraemer
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  2. Christer Magnusson
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Correspondence to Wilfrida Decraemer.

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No specific permits were required for the described fieldwork studies. Permission for sampling was granted by the landowner. The sites are not protected in any way.

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Decraemer, W., Magnusson, C. & Bert, W. Morphological and molecular characterisation of Trichodorus hellalae n. sp. (Nematoda: Trichodoridae) from Norway. Eur J Plant Pathol 161, 91–105 (2021). https://doi.org/10.1007/s10658-021-02306-8

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