Abstract
Blast disease is one of the most important diseases of rice with the fungus Magnaporthe oryzae as the causal agent. This research was carried out to evaluate the blast resistance in aerobic rice genotypes in comparison with Iranian lowland rice cultivars as a randomized complete block design by measuring area under the disease progress curve (AUDPC) on 53 rice genotypes with three replications in 2017 and 2018 years. Furthermore, molecular evaluation was done using 30 genes-based markers and linked SSR markers along with 60 random SSR markers. According to the results, aerobic rice genotypes, IR82310-B-B-67-2, IR82589-B-B-114-3, IR82616-B-B-64-3, IR82589-B-B-84-3, and IR82635-B-B-143–1 (ranged from 4.11 to 5.96) and Iranian rice cultivars Nemat, Sange-Tarom and Sepidroud (ranged from 4.46 to 4.72) had the lowest AUDPC, indicating they had the least infection severity during different stages of the experiment. Iranian native rice cultivars showed the most susceptibility, and association analysis via stepwise regression showed a significant relationship between some of the markers and AUDPC. Two gene-based markers of Pi5 and Pib and RM259 were detected in accordance with previous studies, and eight SSR markers (RM12091, RM217, and RM3498 with positive effect along with RM234, RM276 RM1300, RM8007 and RM480 with negative effect) were identified as informative markers in relationship with AUDPC for the first time. RM12091 and Pi5 had the highest adjusted R2, accounting for 30.2% and 25.2% of AUDPC variation, respectively. The most effective identified markers could be considered in marker-assisted selection after final validation using different environments and genetic backgrounds. In addition, the results of this study can help demonstrate the significant potential of aerobic rice genotypes for resistance to blast disease.
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References
Ashkani, S., Rafii, M. Y., Rusli, I., Sariah, M., Akmar Abdullah, S. N., Abdul Rahim, H., et al. (2012). SSRs for marker-assisted selection for blast resistance in rice (Oryza sativa L.). Plant Molecular Biology Reporter, 30, 79–86.
Bouman, B. A. M. (2001). Water-efficient management strategies in rice production. International Rice Research Institute Notes, 26(2), 17–22.
Cantrell, R. P., & Reeves, T. G. (2002). The rice genome: The cereal of the world’s poor takes center stage. Science, 296, 53.
Cho, Y. C., Kwon, S. W., Choi, I. S., Lee, S. K., Jeon, J. S., & Oh, et al. (2007). Identification of major blast resistance genes in Korean rice cultivars using molecular markers. Journal of Crop Science and Biotechnology, 10, 265–276.
Creste, S., Tulmann Neto, A., & Figueira, A. (2001). Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Molecular Biology Reporter, 19, 299–306.
Eizenga, G. C., Agrama, H. A., Lee, F. N., Yan, W., & Jia, Y. (2006). Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Science, 46, 1870–1878.
Ghiasi, R., Allahyari, M. S., Damalas, C. A., Azizi, J., & Abedi, M. (2017). Crop protection services by plant clinics in Iran: An evaluation through rice farmers' satisfaction. Crop Protection, 98, 191–197.
Hayashi, K., Yoshida, H., & Ashikawa, I. (2006). Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theoretical and Applied Genetics, 113, 251–260.
IBM Corp. (2016). IBM SPSS statistics for windows, version 24.0. Armonk: IBM Corp (released 2016).
Imam, J., Alam, S., Mandal, N. P., Variar, M., & Shukla, P. (2013). Molecular screening for identification of blast resistance genes in north east and eastern Indian rice germplasm (Oryza sativa L.) with PCR based makers. Euphytica, 196(2), 199–211.
Ingole, K. D., Prashanthi, S. K., & Krishnaraj, P. U. (2014). Mining for major blast resistance genes in rice landraces of Karnataka. Indian Journal of Genetics and Plant Breeding, 47, 378–383.
IRRI. (1996). Standard evaluation system for Rice (4th ed.). Los Baños: International Rice Research Institute.
Jamali, S. H., Mohammadi, S. A., & Sadeghzadeh, B. (2017). Association mapping for morphological traits relevant to registration of barley varieties. Spanish Journal of Agricultural Research, 15(4), e0704.
Mousanejad, S., Alizadeh, A., & Safaie, N. (2009). Effect of weather factors on spore population dynamics of blast fungus in Guilan province. Journal of Plant Protection Research, 49(3), 319–329.
Mousanejad, S., Moumeni, A., & Javan-Nikkhah, M. (2010). Evaluation of the blast resistance components in some rice cultivars. Iranian Journal of Plant Pathology, 46(1).
Niknam-Galejugi, M., Salehi, J. G., & Javan-Nikkhah, M. (2014). Characterization and phylogenetic analysis of Magnaporthe spp. strains on various hosts in Iran. Iranian Journal of Biotechnology, 12(3), e1007.
Peng, S., Tang, Q., & Zou, Y. (2009). Current status and challenges of Rice production in China. Plant Production Science, 12, 3–8.
Peterson, L. G. (1990). Tricyclazole for control of Pyricularia Oryzae on Rice: The relationship of the mode of action and disease occurrence and development. In B. T. Grayson, M. B. Green, & L. G. Copping (Eds.), Pest Management in Rice (pp. 122–130). Dordrecht: Springer.
RoyChowdhury, M., Jia, Y., Jackson, A., Jia, M. H., Fjellstrom, R., & Cartwright, R. D. (2012). Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers. Euphytica, 184, 35–46.
Sabouri, A., Afshari, R., Raiesi, T., Babaei Raouf, H., Nasiri, E., Esfahani, M., Kafi Ghasemi, A., & Kumar, A. (2018). Superior adaptation of aerobic rice under drought stress in Iran and validation test of linked SSR markers to major QTLs by MLM analysis across two years. Molecular Biology Reports, 45(5), 1037–1053.
Safari Motlagh, M. R., & Fallahpoor Salkooyeh, F. (2016). Financial comparison of management methods of Rice blast disease in Langerood County, Iran. International Journal of Agricultural Management and Development, 6(3), 291–299.
Saghai-Maroof, M. A., Biyashev, R. M., Yang, G. P., Zhang, Q., & Allard, R. W. (1994). Extraordinarily polymorphic DNA in barely species diversity, choromosomal location, and population dynamics. Proceeding of the National Academy of Science USA, 91(12), 5466–5570.
SAS Institute Inc. (2003). SAS/STAT® User’s Guide, Version 9. Cary: SAS Institute Inc..
Singh, A. K., Singh, P. K., Arya, M., Singh, N. K., & Singh, U. S. (2015). Molecular screening of blast resistance genes in Rice using SSR markers. Plant Pathology Journal, 31, 12–24.
Srivastava, D., Shamim, M., Kumar, M., Mishra, A., Pandey, P., Kumar, D., Yadav, P., Siddiqui, M. H., & Singh, K. N. (2017). Current status of conventional and molecular interventions for blast resistance in Rice. Rice Science, 24, 299–321.
Sun, G. (2012). Distirbutinon of resistance genes in rice and avirulence genes in rice blast fungus and molecule detection of Magnaporthe oryzae. Jinlin: University of Jinlin (in Chinese with English abstract).
Wang, Y., & Li, J. (2005). The plant architecture of rice. Plant Molecular Biology, 59, 75–84.
Wang, X., Lee, S., Wang, J., Ma, J., Bianco, T., & Jia, Y. (2014). Current advances on genetic resistance to Rice blast disease. In W. Yan & J. Bao (Eds.), Rice - Germplasm, Genetics and Improvement (pp. 195–217). Rijeka: In Tech Press.
Wu, Y. Y., He, J. B., Li, A. H., Fang, N. Y., He, W. W., Dang, L. L., et al. (2016). Population structure analysis and association mapping of blast resistance in indica rice (Oryza sativa L.) landraces. Genetics and Molecular Research, 15(3), 1–11.
Yadav, M. K., Aravindan, S., Ngangkham, U., Shubudhi, H. N., Bag, M. K., Adak, T., et al. (2017). Use of molecular markers in identification and characterization of resistance to rice blast in India. PLoS One, 12, 1–19.
Yadav, M. K., Aravindan, S., Ngangkham, U., Raghu, S., Prabhukarthikeyan, S. R., Keerthana, U., et al. (2019). Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers. PLoS One, 14, 1–19.
Yan, L., Bai-yuan, Y., Yun-liang, P., Zhi-juan, J., Yu-xiang, Z., Han-lin, W., et al. (2017). Molecular screening of blast resistance genes in rice germplasm resistant to Magnaporthe oryzae. Rice Science, 24, 41–47.
Zhang, H., Li, G., Li, W., & Song, F. (2009). Transgenic strategies for improving rice disease resistance. African Journal of Biotechnology, 8, 1750–1757.
Zhu, M., Wang, L., & Pan, Q. H. (2004). Identification and characterization of a new blast resistance gene located on rice chromosome 1 through linkage and differential analyses. Phytopathology, 94, 515–519.
Acknowledgments
We would like to acknowledge the help of Dr. A.R. Dadras, at Olive Research Station of Tarom, AREEO, Zanjan, Iran, and Miss Zeinab Mohammadi, PhD Student of University of Zanjan for their technical and scientific assistance. We are also grateful to the University of Guilan for their financial support.
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This research supported by University of Guilan.
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Sabouri, A., Alinezhad, F. & Mousanejad, S. Association analysis using SSR markers and identification of resistant aerobic and Iranian rice cultivars to blast disease. Eur J Plant Pathol 158, 561–570 (2020). https://doi.org/10.1007/s10658-020-02102-w
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DOI: https://doi.org/10.1007/s10658-020-02102-w