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Erwinia persicina as the new causal agent of lettuce soft rot

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Abstract

Water-soaked large spots on leaves, severe rot on the head and tissue collapse symptoms were observed on field-grown lettuce (Lactuca sativa) in one of the most important lettuce growing areas in central Anatolia during 2018–2019. Bacteria were isolated from symptomatic lettuce plants. Pathogenicity tests were conducted on a curly lettuce variety, and the pathogenic strains were examined with biochemical tests, including antibiotic susceptibility, cultivar reactions, reactions to copper-based compounds and 16S rRNA gene sequencing. The isolated bacterial strains were Gram negative, oxidase negative, fermentative, positive for fermentation of sorbitol and negative for D-xylose, and the strain produced a water-soluble pink pigment on nutrient agar. Some of the strains were resistant to different antibiotics. All the strains were resistant to different levels of copper-based compounds. Sumarnas and Calorina were the most susceptible varieties, and Bachus was the least susceptible variety to infection. 16S rRNA sequencing confirmed that the causative bacterium of lettuce soft rot in this study was Erwinia persicina.

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References

  • Anonymous, (2018). TUIK, Bitkisel üretim istatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr. Accessed: 25 September 2019.

  • Ayers, S. H., Rupp, P., & Johnson Jr., W. T. (1919). A study of the alkali-forming bacteria in milk. US Department of Agriculture Bulletin, 782, 1–39.

    Google Scholar 

  • Basim, H., Minsvage, G. V., Stall, R. E., Wang, J. F., Shanker, S., & Jones, J. B. (2005). Characterization of a unique chromosomal copper resistance gene cluster from Xanthomonas campestris pv. vesicatoria. Applied and Environmental Microbiology, 71, 8284–8291.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 36, 493–496.

    Google Scholar 

  • Behlau, F., Canteros, I. B., Minsavage, G. V., Jones, J. B., & Graham, J. H. (2011). Molecular characterization of copper resistance genes from Xanthomonas citri subsp. citri and Xanthomonas alfalfae subsp. Citrumelonis. Applied and Environmental Microbiology, 77, 4089–4096.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Behlau, F., Canteros, B. I., Jones, J. B., & Graham, H. J. (2012). Copper resistance genes from different Xanthomonads and citrus epiphytic bacteria confer resistance to Xanthomonas citri subsp. citri. European Journal of Plant Pathology, 133, 949–963.

    CAS  Google Scholar 

  • Bender, C. L., & Cooksey, D. A. (1986). Indigenous plasmids in Pseudomonas syringae pv. tomato: Conjugative transfer and role in copper reistance. Journal of Bacteriology, 165, 534–541.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Braun-Kiewnick, A., & Sands, D. C. (2001). Pseudomonas. In N. W. Schaad, J. B. Jones, & W. Chun (Eds.), Laboratory guide to identification of plant pathogenic Bacteria (pp. 84–103). St Paul: APS Press.

    Google Scholar 

  • Brenner, D. J., Neto, J. R., Steigerwalt, A. G., & Robbs, C. F. (1994). “Erwinia nulandii” is a subjective synonym of Erwinia persicinus. International Journal of Systematic Bacteriology, 44, 282–284.

    CAS  PubMed  Google Scholar 

  • Cariddi, C., & Sanzani, S. (2013). A severe outbreak of bacterial lettuce soft rot caused by Pectobacterium carotovorum subsp. carotovorum in Apulia (Italy). Journal of Plant Pathology, 95(2), 441–446.

    Google Scholar 

  • Cho, J. J. (1983). Soft rot of crisphead lettuce incited by Erwinia caratovora subsp. caratovora in Hawai. Phytopathology, 73, 1206–1209.

    Google Scholar 

  • Cooksey, D. A. (1990). Genetics of bactericide resistance in plant pathogenic bacteria. Annual Review of Phytopathology, 28, 201–219.

    CAS  Google Scholar 

  • Cooksey, D. A., & Azad, H. R. (1992). Accumulation of copper and other metals by copper-resistant plant-pathogenic and saprophytic pseudomonads. Applied and Environmental Microbiology, 58(1), 274–278.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cooksey, D. A. (1993). Copper uptake and resistance in bacteria. Molecular Microbiology, 7, 1–5.

    CAS  PubMed  Google Scholar 

  • Cooksey, D. A. (1994). Molecular mechanisms of copper resistance and accumulation in bacteria. FEMS Microbiological Reviews, 14, 381–386.

    CAS  Google Scholar 

  • Cottyn, B., Vanhouteghem, K., Heyrman, J., et al. (2005). Pseudomonads associated with midrib rot and soft rot of butterhead lettuce and endive. Communications in Agricultural and Applied Biological Science, 70(3), 101–109.

    CAS  Google Scholar 

  • Davies, J., & Davies, D. (2010). Origins and evolution of antibiotic resistance. Microbiology and Molecular Biology Reviews, 74(3), 417–433.

    CAS  PubMed  Google Scholar 

  • Dever, L. A., & Dermody, T. S. (1991). Mechanisms of bacterial resistance to antibiotics. Archives of internal medicine, 151(5), 886–895.

    CAS  PubMed  Google Scholar 

  • Diánez, F., Santos, M., Font, I., de Cara, M., & Tello, J. C. (2007). New hosts for the enterobacterial phytopathogen Erwinia persicina. In A. Mendez-Vilas (Ed.), Current research topics in applied microbiology and microbial biotechnology (pp. 78–82). Singapore: World Scientific Press, Ltd..

    Google Scholar 

  • Euzéby, J. P. (1998). Taxonomic note: Necessary correction of specific and subspecific epithets according to rules 12c and 13b of the international code of nomenclature of Bacteria (1990 revision). International Journal of Systematic Bacteriology, 48, 1073–1075.

    Google Scholar 

  • FAOSTAT. 2018. "Lettuce (with chicory) production in 2018; countries/regions/production quantity from pick lists". UN Food & Agriculture Organization, Statistics Division (FAOSTAT). Accessed: 13 September 2019.

  • Felix, K. C. S., Oliveira, W. J., Mariano, R. L. R., & Souza, E. B. (2014). Lettuce genotype resistance to "soft rot" caused by Pectobacterium carotovorum subsp. carotovorum. Scientia Agricola, 71(4), 287–291.

    Google Scholar 

  • Gálvez, L., Gil-Serna, J., García-Díaz, M., & Palmero, D. (2015). First report of a garlic bulb rot caused by Erwinia persicina in Europe. Plant Disease, 99, 723.

    Google Scholar 

  • González, A. J., Tello, J. C., & de Cara, M. (2005). First report of Erwinia persicina from Phaseolus vulgaris in Spain. Plant Disease, 89, 109.

    PubMed  Google Scholar 

  • González, A. J., Tello, J. C., & Rodicio, M. R. (2007). Erwinia persicina causing chlorosis and necrotic spots in leaves and tendrils of Pisum sativum in southeastern Spain. Plant Disease, 91, 460.

    PubMed  Google Scholar 

  • Hao, M. V., Brenner, D. J., Steigerwalt, A. G., Kosako, Y., & Komagata, K. (1990). Erwinia persicinus, a new species isolated from plants. International Journal of Systematic Bacteriology, 40, 379–383.

    CAS  PubMed  Google Scholar 

  • Hentschel, U. M., Steinert, M., & Hacker, J. (2000). Common molecular mechanisms of symbiosis and pathogenesis. Trends in Microbiology, 8(5), 236–241.

    Google Scholar 

  • Huang, H. C., Hsieh, T. F., & Erickson, R. S. (2003). Biology and epidemiology of Erwinia rhapontici, causal agent of pink seed and crown rot of plants. Plant Pathology Bulletin, 12, 69–76.

    Google Scholar 

  • Hyman, L. J., Toth, I. K., & Pérombelon, M. C. M. (1998). Methods for the detection and quantification of Erwinia carotovora subsp. atroseptica on potatoes. In M. C. M. Pérombelon & J. M. Van Der Wolf (Eds.), Laboratory manual (pp. 60–65). Invergowrie: Scottish Crop Research Institute.

    Google Scholar 

  • Kim, M., & Chun, J. (2014). 16SrRNA gene-based identification of Bacteria and Archeae using the EZTaxon server. Methods in Microbiology, 41, 61–74.

    CAS  Google Scholar 

  • Koike, S. T., Gladders, P., & Paulus, A. O. (2006). Vegetable diseases: A color handbook. Texas: Gulf Professional Publishing.

    Google Scholar 

  • Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biolology and Evolution, 33, 1870–1874.

    CAS  Google Scholar 

  • Lamichhane, J. R., Osdaghi, E., Behlau, F., Köhl, J., Jones, J. B., & Aubertot, J. N. (2018). Thirteen decades of antimicrobial copper compounds applied in agriculture. A review. Agronomy for Sustainable Development, 38, 28.

    Google Scholar 

  • Marchezi, J. S., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., & Wade, W. G. (1998). Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S-rRNA. Applied Environmental Microbiology, 64, 795–799.

  • Moretti, C., Hosni, T., Vandemeulebroecke, K., Brady, C., De Vos, P., Buonaurio, R., & Cleenwerck, I. (2011). Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. savastanoi. International Journal of Systematic and Evolutionary Microbiology, 61, 2745–2752.

    CAS  PubMed  Google Scholar 

  • Nazerian, E., Sijam, K., Meor Ahmad, Z. A., & Vadamalai, G. (2013). Characterization of Pectobacterium carotovorum subsp. carotovorum as a new disease on lettuce in Malaysia. Australasian Plant Disease Notes, 8, 105–107.

    Google Scholar 

  • Nechwatal, J., & Theil, N. (2019). Erwinia persicina associated with a pink rot of parsley root in Germany. Journal of Plant Diseases and Protection, 126, 161–167.

    Google Scholar 

  • Norman, A., Hansen, L. H., & Sorensen, S. J. (2009). Conjugative plasmids: Vessels of the communal gene pool. Philosophical Transaction of Royal Society of London Serious B. Biological Sciences, 364, 2275–2289.

    CAS  PubMed  Google Scholar 

  • Raina, V., Nayak, T., Ray, L., Kumari, K., & Suar, M. (2019). Approach for designation and description of novel microbial species. In S. Das & H. R. Dash (Eds.), Microbial diversity in the genomic era (pp. 137–152). India: Elsevier.

    Google Scholar 

  • Sahin, F. (2000). First report of bacterial spot of lettuce caused by Xanthomonas campestris pv. vitians in Turkey. Plant Disease, 84, 490.

    CAS  PubMed  Google Scholar 

  • Stackebrandt, E., & Ebers, J. (2006). Taxonomic parameters revisited: Tarnished gold standards. Microbiology Today, 33, 152–155.

    Google Scholar 

  • Stall, R. E., Loschke, D. C. & Jones, J. B. (1986). Linkage of copper resistance and a virulence loci on a selftransmissible plasmid in Xanthomonas campestris pv. vesicatoria. Phytopathology, 76, 240–243

  • Thirthamallappa, S., & Lohithaswa, H. C. (2000). Genetics of resistance to early blight (Alternaria solani Sorauer) in tomato (Lycopersicon esculentum L.). Euphytica, 113, 187–193.

    Google Scholar 

  • Viriyasuthee, W., Saepaisan, S., Saksirirat, W., Gleason, M. L., Chen, R. S., & Jogloy, S. (2019). Effective plant ages for screening for field resistance to Alternaria leaf spot (caused by Alternaria spp.) under natural infection in Jerusalem artichoke (Helianthus tuberosus L.). Agronomy, 9, 754.

    CAS  Google Scholar 

  • Zhang, Z. F., & Nan, Z. B. (2012). First report of Erwinia persicinus causing wilting of Medicago sativa sprouts in China. Plant Disease, 96, 454.

    CAS  PubMed  Google Scholar 

  • Zhang, Z. F., & Nan, Z. B. (2013). Occurrence of lucerne seed-borne Enterobacter cloacaesprouts decay in Gansu Province of China. European Journal of Plant Pathology, 135, 5–9.

    CAS  Google Scholar 

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Acknowledgements

Author thanks to Dr. Jan NECHWATAL for kindly providing the reference E. persicina strains and to Dr. Thomas J. BURR for his helpful advice on the manuscript of this paper.

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  1. Faculty of Agriculture Department of Plant Protection, Ankara University, 06135, Ankara, Turkey

    Didem Canik Orel

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Canik Orel, D. Erwinia persicina as the new causal agent of lettuce soft rot. Eur J Plant Pathol 158, 223–235 (2020). https://doi.org/10.1007/s10658-020-02068-9

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