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Seedling and adult stage resistance to spot form of net blotch (SFNB) in spring barley and stability of adult stage resistance to SFNB in Morocco

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Abstract

The aims of this study were to identify resistance to spot form of net blotch (SFNB) in spring barley and to investigate the stability of SFNB resistance in Morocco. The seedling resistance to SFNB was evaluated by inoculating 340 barley genotypes with the Pyrenophora teres F. maculata (Ptm) isolate FGOB10Ptm-1 (FGO) in the greenhouse. The same barely genotypes were evaluated for adult-stage plant resistance to SFNB in field trials in Morocco. All experiments were conducted in alpha-lattices with two replicates. SFNB disease severity was estimated on barley leaves using double digit scale. To investigate the stability of both qualitative and quantitative resistance to SFNB, 104 barley genotypes were subjected to AMMI analysis. Differential responses of SFNB barley resistance to FGO were found at the seedling stage in the greenhouse. Twelve genotypes showing scores of <1.5, at the seedling stage, were determined to be highly resistant to FGO. The ANOVA showed highly significant (p < 0.001) effects of genotype (G) and G × E (E-Pathotypes of SFNB) interaction on SFNB severity among the 340 barley genotypes at the adult plant stage. The AMMI ANOVA showed that IPCA1, IPCA2 and IPCA3 accounted for 77.9% of the variation of the G × E interaction for SFNB severity. The G × E interaction consisted of divergent genotypic responses to SFNB severity due to different pathotypes prevalent in hot-spot environments. The AMMI stability value demonstrated that barley genotypes AM-14, 30, 31, 68, 107, 108, 112, 149, 170, 185, 204, 240, 304, 326, 326 and 337 were resistant and stable across hot-spot environments against SFNB. Divergent environmental responses of SFNB were recorded in MCH_2015, SE_2015, AT_2016, SE_2016 and JS_2015. Stable SFNB resistant genotypes are valuable resources for the introgression of qualitative and quantitative resistance to barley in Morocco.

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References

  • Abamu, F. J., Akinsola, E. A., & Alluri, K. (1998). Applying the AMMI models to understand genotype-by-environment (G×E) interactions in rice reaction to blast disease in Africa. International Journal of Pest Management, 44(4), 239–245. https://doi.org/10.1080/096708798228167.

    Article  Google Scholar 

  • Aina, O. O., Dixon, A. G. O., & Akinrinde, E. A. (2007). Additive main effects and multiplicative interaction (AMMI) analysis for yield of cassava in Nigeria. Journal of Biological Sciences, 7, 796–800.

    Article  Google Scholar 

  • Amezrou, R., Gyawali, S., Belqadi, L., Chao, S., Arbaoui, M., Mamidi, S., Rehman, S., Sreedasyam, A., & Verma, R. P. S. (2017) Molecular and phenotypic diversity of ICARDA spring barley (Hordeum vulgare L.) collection. Genetic Resour Crop Evol 65:255–269. https://doi.org/10.1007/s10722-017-0527-z

  • Amezrou, R. (2018) Genome-wide association studies of multiple traits in barley (Hordeum vulgare L.). PhD dissertation. Institut Agronomique et Vétérinaire Hassan II. Rabat, Morocco

  • Bentata, F., Labhilili, M., EI Aissami, A., Bary, S., Yeslem, C. H., & Ibijbijen, J. (2011). Analysis of diversity genetic of Moroccan net blotch populations using amplified fragment length polymorphism (AFLP) markers. African Journal of Biotechnology, 10(39), 7548–7554. https://doi.org/10.5897/AJB10.1340.

    Article  CAS  Google Scholar 

  • Cherif, M., Rezgui, S., Devaux, P., & Harrabi, M. (2010). Genotype × environment interactions and heritability of quantitative resistance to net blotch in Tunisian barley. Journal of Plant Breeding and Crop Science, 2(5), 110–116.

    Google Scholar 

  • Cromey, M. G., & Parkes, R. A. (2003). Pathogenic variation in Drechslera teres in New Zealand. New Zealand Plant Protection, 56, 251–256.

    Google Scholar 

  • Crossa, J., Cornelius, P. L., & Yan, W. (2002). Biplots of linear bilinear models for studying crossover genotypes x environment interaction. Crop Science, 42, 619–633. https://doi.org/10.1111/j.1439-0434.2009.01631.x.

    Article  CAS  Google Scholar 

  • DeLacy, I. H., Basford, K. E., Cooper, M., & Fox, P. N. (1996a). Retrospective analysis of historical data sets from multi-environment trials- theoretical development. In M. Cooper & G. L. Hammer (Eds.), Plant adaptation and crop improvement (pp. 243–267). Wallingford, UK: CAB International.

    Google Scholar 

  • DeLacy, I. H., Ratnasiri, W. G. A., & Mirzawan, P. D. N. (1996b). Retrospective analysis of historical data sets from multi-environment trials-case studies. In M. Cooper & G. L. Hammer (Eds.), Plant adaptation and crop improvement (pp. 269–290). Wallingford, UK: CAB International.

    Google Scholar 

  • Douiyssi, A., Rasmusson, D. C., & Roelfs, A. P. (1998). Responses of barley cultivars and lines to isolates of Pyrenophora teres. Plant Disease, 82, 316–321.

    Article  CAS  PubMed  Google Scholar 

  • Eberhart, S. A., & Russell, W. A. (1966). Stability parameters for comparing varieties. Crop Science, 6, 36–40.

    Article  Google Scholar 

  • Eyal Z, Scharen AL, Prescott JM, Van Ginkel M (1987) The Septoria diseases of wheat: Concepts 483 and methods of disease management. CIMMYT, Mexico D.F.

  • Farshadfar, E., Mahmodi, N., & Yaghotipoor, A. (2011). AMMI stability value and simultaneous estimation of yield and yield stability in bread wheat (Triticum aestivum L.). Australian Journal of Crop Science, 5(13), 1837–1844.

    Google Scholar 

  • Finlay, K. W., & Wilkinson, G. N. (1963). The analysis of adaptation in a plant breeding programme. Australian Journal of Agricultural Research, 14, 742–754.

    Article  Google Scholar 

  • Forbes, G. A., Chacón, M. G., Kirk, H. G., Huarte, M. A., Van Damme, M., Distel, S., Mackay, G. R., Stewart, H. E., Lowe, R., Duncan, J. M., Mayton, H. S., Fry, W. E., Andrivon, D., Ellissèche, D., Pellé, R., Platt, H. W., Mackenzie, G., Tarn, T. R., Colon, L. T., Budding, D. J., Lozoya-Saldaña, H., Hernandez-Vilchis, A., & Capezio, S. (2005). Stability of resistance to Phytophthora infestans in potato: An international evaluation. Plant Pathology, 54(3), 364–372. https://doi.org/10.1111/j.1365-3059.2005.01187.x.

    Article  Google Scholar 

  • Gauch, H. G., Piepho, H. P., & Annicchiarico, P. (2008). Statistical analysis of yield trials by AMMI and GGE: Further considerations. Crop Science, 48, 866–889. https://doi.org/10.2135/cropsci2007.09.0513.

    Article  Google Scholar 

  • Gupta, S., & Loughman, R. (2001). Current virulence of Pyrenophora teres on barley in Western Australia. Plant Disease, 85, 960–966.

    Article  PubMed  Google Scholar 

  • Harrabi, M., & Kamel, A. (1990). Virulence spectrum to barley in some isolates of Pyrenophora teres from the Mediterranean region. Plant Disease, 74, 230–232.

    Article  Google Scholar 

  • Jebbouj, R., & El Yousfi, B. (2010). An integrated multivariate approach to net blotch of barley: Virulence quantification, pathotyping and a breeding strategy for disease resistance. European Journal Plant Pathology, 127, 521–544.

    Article  Google Scholar 

  • Jonsson, R., Bryngelssonm, T., & Gustafsson, M. (1997). Virulence studies of Swedish net blotch isolates (Drechslera teres) and identification of resistant barley lines. Euphytica, 94, 209–218.

    Article  Google Scholar 

  • Kinzer KMD (2015) Characterizing P. teres F. maculata in the northern United States and impact of spot form net blotch on yield of barley. PhD Dissertation. North Dakota State University, ND, USA.

  • Liu, Z. H., Zhong, S., Stasko, A. K., Edwards, M. C., & Friesen, T. L. (2012). Virulence profile and genetic structure of a North Dakota population of Pyrenophora teres f. teres, the causal agent of net form net blotch of barley. Phytopathology, 102, 539–546.

    Article  CAS  PubMed  Google Scholar 

  • Mathre, D. E. (1982). Compendium of barley diseases. Am. Phytopathol. Soc. St. In Paul. USA: Minnesota.

    Google Scholar 

  • Miranda, G. V., Souza, L. V., Guimarães, L. J. M., Namorato, H. L., Oliveira, R., & Soares, M. O. (2009). Multivariate analyses of genotype x environment interaction of popcorn. Pesquisa Agropecuária Brasileira, 44(1), 45–50.

    Article  Google Scholar 

  • Neupane, A., Tamang, P., Brueggeman, R. S., & Friesen, T. L. (2015). Evaluation of barley core collection for spot form of net blotch reaction reveals distinct genotype-specific pathogen virulence and host susceptibility. Phytopathology, 105, 509–517. https://doi.org/10.1094/PHYTO-04-14-0107-R.

    Article  CAS  PubMed  Google Scholar 

  • Perkins, J. M., & Jinks, J. L. (1968). Environmental and genotype environmental components of variability. III. Multiple lines and crosses. Heredity, 23, 339–356.

    Article  CAS  PubMed  Google Scholar 

  • Qamar, A., Liu, Z.H.M., Faris, J.D., Chao, S., Edwards, M.C., Lai, Z., Franckowiak, J.D., Friesen, & T.L. (2008). A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance. Theoretical and Applied Genetics, 117, 1261–1270.

  • Rao, P. S., Reddy, P. S., Rathore, A., Reddy, B. V., & Panwar, S. (2011). Application GGE biplot and AMMI model to evaluate sweet sorghum (Sorghum bicolor) hybrids for genotype x environment interaction and seasonal adaptation. Indian Journal of Agricultural Science, 81, 438–444.

    Google Scholar 

  • Robinson, J., & Jalli, M. (1999). Sensitivity of resistance to net blotch in barley. Journal of Phytopathology, 147(4), 235–241. https://doi.org/10.1046/j.1439-0434.1999.147004235.x.

    Article  Google Scholar 

  • Rose, L. J., Mouton, M., Beukes, I., Flett, B. C., van der Vyver, C., & Viljoen, A. (2016). Multi-environment evaluation of maize inbred lines for resistance to fusarium ear rot and fumonisins. Plant Disease, 100(10), 2134–2144. https://doi.org/10.1094/PDIS-11-15-1360-RE.

    Article  CAS  PubMed  Google Scholar 

  • Saari, E. E., & Prescott, J. M. (1975). A scale for appraising the foliar severity of wheat diseases. Plant Disease Reporter, 59, 377–380.

    Google Scholar 

  • Shah, S. J. A., Imtiaz, M., & Hussain, S. (2010). Phenotypic and molecular characterization of wheat for slow rusting resistance against Puccinia striiformis Westend. f.sp. tritici. Journal of Phytopathology, 158(6), 393–402. https://doi.org/10.1111/j.1439-0434.2009.01631.x.

    Article  CAS  Google Scholar 

  • Shipton, W. A., Khan, T. N., & Boyd, W. J. R. (1973). Net blotch of barley. Review of Plant Pathology, 52, 269–290.

    Google Scholar 

  • Shukla, G. K. (1972). Some statistical aspects of partitioning genotype-environment components of variability. Heredity, 29, 237–245.

    Article  CAS  PubMed  Google Scholar 

  • Taibi, K., Bentata, G., Rehman, S., Labhilili, M., El Aissami, A., Verma, R. P. S., & Gyawali, S. (2016) Virulence of Moroccan Pyrenophora teres f. teres revealed by international differential barley genotypes. 44(2):263–271

  • Tamang, P., Neupane, A., Mamidi, S., Friesen, T., & Brueggeman, R. (2015). Association mapping of seedling resistance to spot form of net blotch in a worldwide collection of barley. Phytopathology, 105, 500–508.

    Article  CAS  PubMed  Google Scholar 

  • Tekauz, A. (1990). Characterization and distribution of pathogenic variation in Pyrenophora teres f. teres and P. teres f. maculata from western Canada. Canadian Journal of Plant Pathology, 12, 141–148.

    Article  Google Scholar 

  • Tuohy, J. M., Jalli, M., Cooke, B. M., & O’Sullivan, E. (2006). Pathogenic variation in populations of Drechslera teres f. teres and D. teres f. maculata and differences in host cultivar responses. European Journal of Plant Pathology, 116, 177–185. https://doi.org/10.1007/s10658-006-9001-z.

    Article  Google Scholar 

  • Yan, W., Hunt, L. A., Sheng, Q., & Szlavnics, Z. (2000). Cultivar evaluation and mega-environment investigation based on GGE biplot. Crop Science, 40, 596–605.

    Article  Google Scholar 

  • Yousfi, B. E., & Ezzahiri, B. (2001). Net blotch in semi-arid regions of Morocco. I. Epidemiology. Field Crop Research, 73, 35–46. https://doi.org/10.1016/S0378-4290(01)00180-0.

    Article  Google Scholar 

  • Yousfi, B. E., & Ezzahiri, B. (2002). Net blotch in semi-arid regions of Morocco II. Yield and yield-loss modeling. Field. Crop Research, 73, 81–93. https://doi.org/10.1016/S0378-4290(01)00189-7.

    Article  Google Scholar 

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Acknowledgements

The authors are thankful to the research technicians of ICARDA and INRA-Maroc in all of the test locations. The funding for this research was provided jointly by ICARDA and CRP-Dryland Cereals. Authors are thankful to Mr. Alex Marcus Batson from Washington State University, NWREC, Mt. Vernon for his critical review and English editing.

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  1. Sanjaya Gyawali

    Present address: Washington State University, NWREC, 16650 State Route 536, Mt Vernon, WA, 98273, USA

Authors and Affiliations

  1. International Center for Agricultural Research in the Dry Areas (ICARDA), Crop Improvement Program, BIGM Program, Rabat, Morocco

    Sanjaya Gyawali, Reda Amezrou, Ramesh Pal Singh Verma, Sajid Rehman & Murari Singh

  2. Department of Plant Pathology, North Dakota State University, Walster Hall, Fargo, ND, 58102, USA

    Robert Brueggeman & Prabin Tamang

  3. IAV Hassan II, Department of Plant Breeding and Genetics, Rabat, Morocco

    Loubna Belqadi & Mustapha Arbaoui

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  1. Sanjaya Gyawali
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Correspondence to Sanjaya Gyawali.

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Gyawali, S., Amezrou, R., Verma, R.P.S. et al. Seedling and adult stage resistance to spot form of net blotch (SFNB) in spring barley and stability of adult stage resistance to SFNB in Morocco. Eur J Plant Pathol 153, 475–487 (2019). https://doi.org/10.1007/s10658-018-1575-8

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