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Incidence and abundance of Verticillium dahliae in soil from various agricultural fields in Prince Edward Island, Canada

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Abstract

Verticillium dahliae causes wilt in potatoes and it is associated with the potato early dying syndrome. Previous studies showed that V. dahliae is present in Prince Edward Island, a major potato producing province in Canada; however, its current incidence and abundance are largely unknown. In recent years an increase in the frequency of V. dahliae-related diseases was observed in the province, affecting tuber yields. To assess the distribution of V. dahliae in this region, a survey of the incidence and abundance of this pathogen was carried out using real -time quantitative PCR. Ten fields, from the major potato production areas, cultivated with potato or with rotating crops, were investigated for two successive years. Analysis by qPCR of DNA extracted from over 200 soil samples revealed that V. dahliae has a high incidence in the province with an uneven distribution in the different locations analyzed and over the two growing seasons, while crop rotation provided a limited control of the pathogen.

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Acknowledgements

The research was funded by PEI Agriculture Research and Innovation Fund Project number ARP019.

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Authors and Affiliations

  1. Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS, B2N 5E3, Canada

    Tudor Borza, Anjana Govindarajan, Xingxi Gao, Yahui Liu & Gefu Wang-Pruski

  2. PEI Department of Agriculture and Forestry, Charlottetown, PE, C1A 7N8, Canada

    Brian Beaton

  3. Cavendish Farms, New Annan, PE, C1N 5J5, Canada

    Zenaida Ganga

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  1. Tudor Borza
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  2. Brian Beaton
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  3. Anjana Govindarajan
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  4. Xingxi Gao
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  5. Yahui Liu
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  6. Zenaida Ganga
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  7. Gefu Wang-Pruski
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Correspondence to Gefu Wang-Pruski.

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This work was not submitted for publication to another journal. All authors listed have contributed to the work, have read the manuscript and declare that there are no potential conflicts of interest.

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Borza, T., Beaton, B., Govindarajan, A. et al. Incidence and abundance of Verticillium dahliae in soil from various agricultural fields in Prince Edward Island, Canada. Eur J Plant Pathol 151, 825–830 (2018). https://doi.org/10.1007/s10658-017-1408-1

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  • DOI: https://doi.org/10.1007/s10658-017-1408-1

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