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Antifungal activity of Boerhavia diffusa L. extract against Phytophthora spp. in tomato and pepper

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Abstract

Antifungal properties of Boerhavia diffusa L. (Nyctaginaceae) methanolic extract have been investigated against Phytophthora infestans and Phytophthora capsici, causal agents of late blight disease in tomato and pepper, respectively. The minimum inhibitory concentration of B. diffusa extract was 0.25 % for P. infestans and 0.5 % for P. capsici. The extract had fungitoxic effect on P. infestans and completely inhibited its mycelial growth at all tested concentrations (0.25, 0.5, and 1 %). P. capsici mycelial growth was inhibited of 90 % by the 1 % extract after five days of culture. B. diffusa extract, at the concentration of 0.25 % significantly reduced P. infestans disease symptoms also in young tomato plants. The plant extract significantly reduced disease symptoms of P. capsici in young pepper plants at all tested concentrations (0.25, 0.5, and 1 %). The efficacy of B. diffusa extract against P. capsici increased with the increasing extract concentration and decreased over time. The 1 % concentration showed the best efficacy in controlling this oomycete. As its effect was comparable to that of the synthetic fungicide, B. diffusa extract can be considered as a potential environmentally friendly alternative to chemical control of late blight in tomato and pepper.

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References

  • Abou-Jawdah, Y., Sobh, H., & Salameh, A. (2002). Antimycotic activities of selected plant flora, growing wild in Lebanon, against phytopathogenic fungi. Journal of Agricultural and Food Chemistry, 50(11), 3208–3213.

    Article  CAS  PubMed  Google Scholar 

  • Agrawal, A., & Srivastava, M. M. (2008). Structural elucidation of bioactive principle of root of Boerhavia diffusa for its antifungal properties. National Academy Science Letters, 31(7–8), 215–219.

    CAS  Google Scholar 

  • Apu, A. S., Liza, M. S., Jamaluddin, A. T. M., Howlader, M. A., Saha, R. K., Rizwan, F., & Nasrin, N. (2012). Phytochemical screening and in vitro bioactivities of the extracts of aerial part of Boerhavia diffusa Linn. Asian Pacific Journal of Tropical Biomedicine, 2(9), 673–678.

    Article  PubMed  PubMed Central  Google Scholar 

  • Awasthi, L. P., & Verma, H. N. (2006). Boerhaavia diffusa - a wild herb with potent biological and antimicrobial properties. Asian Agri-History, 10(1), 55–68.

    Google Scholar 

  • Bajpai, V. K., & Kang, S. C. (2012). In vitro and in vivo inhibition of plant pathogenic fungi by essential oil and extracts of Magnolia liliflora desr. Journal of Agricultural Science and Technology, 14(4), 845–856.

    Google Scholar 

  • Bajpai, V. K., Lee, T. J., & Kang, S. C. (2009). Chemical composition and in vitro control of agricultural plant pathogens by the essential oil and various extracts of Nandina domestica Thunb. Journal of the Science of Food and Agriculture, 89(1), 109–116.

    Article  CAS  Google Scholar 

  • Baka, Z. A. M. (2014). Antifungal activity of extracts from five Egyptian wild medicinal plants against late blight disease of tomato. Archives of Phytopathology and Plant Protection, 47(16), 1988–2002.

    Article  Google Scholar 

  • Bharathi, K., Thirumurugan, V., Kavitha, M., Muruganandam, G., & Sethuraman, M. (2014). Inter-specific variation studies on the phyto-constituents of Boerhaavia diffusa L. And Cichorium intybus L. Using phytochemical methods. International Journal of Pharmaceutical Sciences Review and Research, 27(1), 283–287.

    Google Scholar 

  • Bhardwaj, R., Yadav, A., & Sharma, R. (2014). Phytochemicals and antioxidant activity in Boerhavia diffusa. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), 344–348.

    Google Scholar 

  • Bosland, P. W., & Lindsey, D. (1991). A seedling screen for Phytophthora root rot of pepper. Capsicum annuum. Plant Dis., 75, 1048–1050.

    Article  Google Scholar 

  • Crinò, P. (1993). Phytophthora infestans/Patata, pomodoro. In P. Crinò, A. Sonnino, F. Saccardo, M. Buiatti, A. Porta-Puglia, & G. Surico (Eds.), Miglioramento genetico delle piante per resistenza a patogeni e parassiti (pp. 310–335). Bologna, Italia: Edagricole.

    Google Scholar 

  • Curtis, H., Noll, U., Störmann, J., & Slusarenko, A. J. (2004). Broad-spectrum activity of the volatile phytoanticipin allicin in extracts of garlic (Allium sativum L.) against plant pathogenic bacteria, fungi and oomycetes. Physiological and Molecular Plant Pathology, 65(2), 79–89.

    Article  CAS  Google Scholar 

  • Damian Badillo, L. M., Martinez Muñoz, R. E., Salgado Garciglia, R., & Martinez Pacheco, M. M. (2010). In vitro antioomycete activity of Artemisia ludoviciana extracts against Phytophthora spp. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 9(2), 136–142.

    Google Scholar 

  • Demirci, F., & Dolar, F. S. (2006). Effects of some plant materials on Phytophthora blight (Phytophthora capsici Leon.) of pepper. Turkish Journal of Agriculture and Forestry, 30(4), 247–252.

    Google Scholar 

  • Goufo, P., Fontem, D. A., & Ngnokam, D. (2010). Evaluation of plant extracts for tomato late blight control in Cameroon. New Zealand Journal of Crop and Horticultural Science, 38(3), 171–176.

    Article  Google Scholar 

  • Harborne, J. B. (1998). Phytochemical methods: a guide to modern techniques of plant analysis (third ed.). London: Chapman & Hall Pub.

    Google Scholar 

  • Khalid, M., Siddiqui, H. H., & Freed, S. (2012). Pharmacognostical evaluation and qualitative analysis of Boerhaavia diffusa L. Roots. International Journal of Pharma and Bio Sciences, 3(1), P16-P23.

  • Lohani, S., Jan, A., & Verma, H. N. (2007). In vivo and in vitro resistance induction in tobacco by Boerhaavia diffusa systemic resistance inducing protein and transfer of induced resistance in in vitro tobacco plants. Biotechnology, 6(3), 389–392.

    Article  CAS  Google Scholar 

  • Madhuri, S., Kalasker, V., Rambhimaiah, & Sreekantha (2013). Evaluation of diuretic activity of aqueous extract of Boerhaavia diffusa roots in rats. International Journal of Pharma and Bio Sciences, 4(4), P843-P848.

  • Monali, P., & Ramtej, V. (2014). Hepatoprotective activity of Boerhavia diffusa extract. International Journal of Pharmaceutical and Clinical Research, 6(3), 233–240.

    Google Scholar 

  • Mone, M., Saieed, M. A. U., Dastogeer, K. M. G., Ali, M. A., & Meah, M. B. (2014). Plumieride from Allamanda cathartica as an inhibitory compound to plant pathogenic fungi. Archives of Phytopathology and Plant Protection, 47(11), 1311–1326.

    Article  CAS  Google Scholar 

  • Ngadze, E. (2014). In vitro and greenhouse evaluation of botanical extracts for antifungal activity against Phythopthora infestans. Journal of Biopesticides, 7(2), 199–204.

    Google Scholar 

  • Oboh, I., Akerele, J., & Obasuyi, O. (2007). Antimicrobial activity of the ethanol extract of the aerial parts of Sida acuta Burm Malvaceae. Tropical Journal of Pharmaceutical Research, 6, 809–813.

    Google Scholar 

  • Osorio, E., Flores, M., Hernández, D., Ventura, J., Rodríguez, R., & Ahuilar, C. N. (2010). Biological efficiency of polyphenolic extracts from pecan nuts shell (Carya Illinoensis), pomegranate husk (Punica granatum) and creosote bush leaves (Larrea tridentata Cov.) against plant pathogenic fungi. Industrial Crops and Products, 31(1), 153–157.

    Article  Google Scholar 

  • Pang, Z., Shao, J. P., Hu, J., Chen, L., Wang, Z., Qin, Z., & Liu, X. (2014). Competition between pyrimorph-sensitive and pyrimorph-resistant isolates of Phytophthora capsici. Phytopathology, 104(3), 269–274.

    Article  CAS  PubMed  Google Scholar 

  • Park, H. J., Lee, J. Y., Moon, S. S., & Hwang, B. K. (2003). Isolation and anti-oomycete activity of nyasol from Anemarrhena asphodeloides rhizomes. Phytochemistry, 64(5), 997–1001.

    Article  CAS  PubMed  Google Scholar 

  • Patel, M., Verma, R., & Srivastav, P. (2014). Antioxidant activity of Boerhavia diffusa extract. International Journal of Pharmacognosy and Phytochemical Research, 6(3), 598–605.

    Google Scholar 

  • Pomerantz, A., Cohen, Y., Shufan, E., Ben-Naim, Y., Mordechai, S., Salman, A., & Huleihel, M. (2015). Characterization of Phytophthora infestans resistance to mefenoxam using FTIR spectroscopy. Journal of Photochemistry and Photobiology B: Biology, 141, 308–314.

    Article  Google Scholar 

  • Rodino, S., Cornea, C. P., Butu, A., & Butu, M. (2014). Control of tomato late blight with Artemisia absinthium extracts. Acta Horticulturae, 1041. doi:10.17660/ActaHortic.2014.1041.26.

  • Rosado-Álvarez, C., Molinero-Ruiz, L., Rodríguez-Arcos, R., & Basallote-Ureba, M. J. (2014). Antifungal activity of asparagus extracts against phytopathogenic Fusarium oxysporum. Scientia Horticulturae, 171, 51–57.

  • Sang, M. K., Kim, J. G., & Kim, K. D. (2010). Biocontrol activity and induction of systemic resistance in pepper by compost water extracts against Phytophthora capsici. Phytopathology, 100(8), 774–783.

    Article  PubMed  Google Scholar 

  • Saraswati, S., Alhaider, A. A., & Agrawal, S. S. (2013). Punarnavine, an alkaloid from Boerhaavia diffusa exhibits anti-angiogenic activity via downregulation of VEGF in vitro and in vivo. Chemico-Biological Interactions, 206(2), 204–213.

    Article  CAS  PubMed  Google Scholar 

  • Singh, A. (2007). Boerhavia diffusa: An-overexploited plant of medicinal importance in eastern Uttar Pradesh. Current Science 93(4), p. 446.

  • Singh, S., Awasthi, L. P., & Singh, R. K. (2011). Induction of systemic resistance through antiviral agents of plant origin against papaya ring spot disease (Carica papaya L.). Archives of Phytopathology and Plant Protection, 44(17), 1676–1982.

    Article  Google Scholar 

  • Švecová, E., & Crinò, P. (2013). Plant-derived biofungicides: overview and perspectives. In M. N. Wheeler & B. R. Johnston (Eds.), Fungicides: classification, role in disease management and toxicity effects (pp. 1–38). New York: Nova Science Publishers.

    Google Scholar 

  • Švecová, E., Proietti, S., Caruso, C., Colla, G., & Crinò, P. (2013). Antifungal activity of Vitex agnus-castus extract against Pythium ultimum in tomato. Crop Protection, 43, 223–230.

    Article  Google Scholar 

  • Tamuli, P., Das, J., & Boruah, P. (2014). Antifungal activity of Vitex negundo Linn. against some phytopathogenic fungi. Plant Archives, 14(2), 981–982.

  • Wagh, S., & Vidhale, N. N. (2010). Antimicrobial efficacy of Boerhaavia diffusa against some human pathogenic bacteria and fungi. Biosciences, Biotechnology Research Asia, 7(1), 267–272.

    Google Scholar 

  • Wei, S., Wu, W., & Ji, Z. (2012). New antifungal pyranoisoflavone from Ficus tikoua bur. International Journal of Molecular Sciences, 13(6), 7375–7382.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Widmer, T. L., & Laurent, N. (2006). Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp. pathogenic to Theobroma cacao. European Journal of Plant Pathology, 115(4), 377–388.

    Article  CAS  Google Scholar 

  • Zhang, L., Hua, Z., Song, Y., & Feng, C. (2014). Monoterpenoid indole alkaloids from Alstonia rupestris with cytotoxic, antibacterial and antifungal activities. Fitoterapia, 97, 142–147.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work is a part of Eva Švecová’s PhD program in Horticulture at University of Tuscia, Viterbo, in collaboration with ENEA C.R. Casaccia, Rome, Italy.

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Authors and Affiliations

  1. Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Lesní 322, 25243, Průhonice, Czech Republic

    Eva Švecová

  2. Department of Agricultural and Forestry Sciences, University of Tuscia, Via S. Camillo de Lellis snc, 01100, Viterbo, Italy

    Eva Švecová & Giuseppe Colla

  3. ENEA C.R. Casaccia, Unit Sustainable Development and Innovation of the Agro-Industrial System, Via Anguillarese 301, 00123, Rome, Italy

    Paola Crinò

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  1. Eva Švecová
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  2. Giuseppe Colla
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Correspondence to Eva Švecová.

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Švecová, E., Colla, G. & Crinò, P. Antifungal activity of Boerhavia diffusa L. extract against Phytophthora spp. in tomato and pepper. Eur J Plant Pathol 148, 27–34 (2017). https://doi.org/10.1007/s10658-016-1065-9

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